Detection and Analysis of FAT10 Modification

Kurzanzeige
dc.contributor.authorAichem, Annette
dc.contributor.authorGroettrup, Marcus
dc.contributor.editorDohmen, R. Jürgen
dc.contributor.editorScheffner, Martin
dc.date.accessioned2013-02-27T09:30:19Zdeu
dc.date.available2013-02-27T09:30:19Zdeu
dc.date.issued2012
dc.description.abstractFAT10 plays a role in many cellular processes. Nevertheless, only one substrate could be identified so far to which FAT10 becomes covalently attached via a nonreducible isopeptide bond. The identification of additional substrates as well as interaction partners is therefore of great interest. Due to the absence of potent anti-FAT10 antibodies, the detection and identifi cation of interaction partners was hindered so far. We have recently described the generation of a novel monoclonal FAT10 antibody that is suitable for immunoprecipitation of endogenous FAT10 and describe in this chapter the detection of endogenous as well as of His-3×FLAG-tagged FAT10 interacting proteins and conjugates by immunoprecipitation using either this novel anti-FAT10 antibody 4FI or an anti-FLAG affinity gel.eng
dc.description.versionpublished
dc.identifier.doi10.1007/978-1-61779-474-2_7deu
dc.identifier.urihttp://kops.uni-konstanz.de/handle/123456789/22005
dc.language.isoengdeu
dc.legacy.dateIssued2013-02-27deu
dc.rightsterms-of-usedeu
dc.rights.urihttps://rightsstatements.org/page/InC/1.0/deu
dc.subjectFAT10deu
dc.subjectFAT10 interacting proteindeu
dc.subjectProteasomal degradationdeu
dc.subjectMonoclonal anti-FAT10 antibody 4FIdeu
dc.subjectImmunoprecipitationdeu
dc.subject.ddc570deu
dc.titleDetection and Analysis of FAT10 Modificationeng
dc.typeINCOLLECTIONdeu
dspace.entity.typePublication
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  publisher={Humana Press},
  address={New York, NY; Heidelberg [u.a.]},
  series={Methods in molecular biology},
  booktitle={Ubiquitin family modifiers and the proteasome : reviews and protocols},
  pages={125--132},
  editor={Dohmen, R. Jürgen},
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kops.citation.iso690AICHEM, Annette, Marcus GRÖTTRUP, DOHMEN, R. Jürgen, ed., Martin SCHEFFNER, ed., 2012. Detection and Analysis of FAT10 Modification. In: DOHMEN, R. Jürgen, ed. and others. Ubiquitin family modifiers and the proteasome : reviews and protocols. New York, NY; Heidelberg [u.a.]: Humana Press, 2012, pp. 125-132. Methods in molecular biology. 832. ISBN 978-1-61779-473-5. Available under: doi: 10.1007/978-1-61779-474-2_7deu
kops.citation.iso690AICHEM, Annette, Marcus GRÖTTRUP, DOHMEN, R. Jürgen, ed., Martin SCHEFFNER, ed., 2012. Detection and Analysis of FAT10 Modification. In: DOHMEN, R. Jürgen, ed. and others. Ubiquitin family modifiers and the proteasome : reviews and protocols. New York, NY; Heidelberg [u.a.]: Humana Press, 2012, pp. 125-132. Methods in molecular biology. 832. ISBN 978-1-61779-473-5. Available under: doi: 10.1007/978-1-61779-474-2_7eng
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kops.sourcefieldDOHMEN, R. Jürgen, ed. and others. <i>Ubiquitin family modifiers and the proteasome : reviews and protocols</i>. New York, NY; Heidelberg [u.a.]: Humana Press, 2012, pp. 125-132. Methods in molecular biology. 832. ISBN 978-1-61779-473-5. Available under: doi: 10.1007/978-1-61779-474-2_7deu
kops.sourcefield.plainDOHMEN, R. Jürgen, ed. and others. Ubiquitin family modifiers and the proteasome : reviews and protocols. New York, NY; Heidelberg [u.a.]: Humana Press, 2012, pp. 125-132. Methods in molecular biology. 832. ISBN 978-1-61779-473-5. Available under: doi: 10.1007/978-1-61779-474-2_7deu
kops.sourcefield.plainDOHMEN, R. Jürgen, ed. and others. Ubiquitin family modifiers and the proteasome : reviews and protocols. New York, NY; Heidelberg [u.a.]: Humana Press, 2012, pp. 125-132. Methods in molecular biology. 832. ISBN 978-1-61779-473-5. Available under: doi: 10.1007/978-1-61779-474-2_7eng
kops.submitter.emailbrigitte.schanze@uni-konstanz.dedeu
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source.titleUbiquitin family modifiers and the proteasome : reviews and protocolseng

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