Publikation:

Detection and Analysis of FAT10 Modification

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2012

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Dohmen, R. Jürgen
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http://nbn-resolving.de/urn:nbn:de:bsz:352-220053
DOI (zitierfähiger Link)
https://doi.org/10.1007/978-1-61779-474-2_7
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Biologie: Publikationen
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DOHMEN, R. Jürgen, ed. and others. Ubiquitin family modifiers and the proteasome : reviews and protocols. New York, NY; Heidelberg [u.a.]: Humana Press, 2012, pp. 125-132. Methods in molecular biology. 832. ISBN 978-1-61779-473-5. Available under: doi: 10.1007/978-1-61779-474-2_7

Zusammenfassung

FAT10 plays a role in many cellular processes. Nevertheless, only one substrate could be identified so far to which FAT10 becomes covalently attached via a nonreducible isopeptide bond. The identification of additional substrates as well as interaction partners is therefore of great interest. Due to the absence of potent anti-FAT10 antibodies, the detection and identifi cation of interaction partners was hindered so far. We have recently described the generation of a novel monoclonal FAT10 antibody that is suitable for immunoprecipitation of endogenous FAT10 and describe in this chapter the detection of endogenous as well as of His-3×FLAG-tagged FAT10 interacting proteins and conjugates by immunoprecipitation using either this novel anti-FAT10 antibody 4FI or an anti-FLAG affinity gel.

Zusammenfassung in einer weiteren Sprache

Fachgebiet (DDC)
570 Biowissenschaften, Biologie

Schlagwörter

FAT10, FAT10 interacting protein, Proteasomal degradation, Monoclonal anti-FAT10 antibody 4FI, Immunoprecipitation

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ISO 690AICHEM, Annette, Marcus GRÖTTRUP, DOHMEN, R. Jürgen, ed., Martin SCHEFFNER, ed., 2012. Detection and Analysis of FAT10 Modification. In: DOHMEN, R. Jürgen, ed. and others. Ubiquitin family modifiers and the proteasome : reviews and protocols. New York, NY; Heidelberg [u.a.]: Humana Press, 2012, pp. 125-132. Methods in molecular biology. 832. ISBN 978-1-61779-473-5. Available under: doi: 10.1007/978-1-61779-474-2_7
BibTex
@incollection{Aichem2012Detec-22005,
  year={2012},
  doi={10.1007/978-1-61779-474-2_7},
  title={Detection and Analysis of FAT10 Modification},
  number={832},
  isbn={978-1-61779-473-5},
  publisher={Humana Press},
  address={New York, NY; Heidelberg [u.a.]},
  series={Methods in molecular biology},
  booktitle={Ubiquitin family modifiers and the proteasome : reviews and protocols},
  pages={125--132},
  editor={Dohmen, R. Jürgen},
  author={Aichem, Annette and Gröttrup, Marcus}
}
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