Rapid screening of lectins for multivalency effects with a glycodendrimer microarray

Kurzanzeige
dc.contributor.authorPera, Núria Pareradeu
dc.contributor.authorBranderhorst, Hilbert M.deu
dc.contributor.authorKooij, Raymonddeu
dc.contributor.authorMaierhofer, Carolinedeu
dc.contributor.authorLiskamp, Rob M. J.deu
dc.contributor.authorWittmann, Valentin
dc.contributor.authorRuijtenbeek, Robdeu
dc.contributor.authorPieters, Roland J.deu
dc.contributor.authorKaaden, Marjolein van derdeu
dc.date.accessioned2011-06-17T07:06:03Zdeu
dc.date.available2012-08-31T22:25:03Zdeu
dc.date.issued2010deu
dc.description.abstractMultivalency is an important phenomenon in protein–carbohydrate interactions. In order to evaluate glycodendrimers as multivalent inhibitors of carbohydrate binding proteins, we displayed them on a microarray surface. Valencies were varied from 1 to 8, and corrections were made for the valencies so that all surfaces contained the same amount of the sugar ligand. Five different carbohydrates were attached to the dendrimers. A series of fluorescent lectins was evaluated, and for each of them a binding profile was obtained from a single experiment showing both the specificity of the lectin for a certain sugar and whether it prefers multivalent ligands or not. Very distinct binding patterns were seen for the various lectins. The results were rationalized with respect to the interbinding distances of the lectins.eng
dc.description.versionpublished
dc.identifier.citationFirst publ. in: ChemBioChem 11 (2010), 13, pp. 1896–1904deu
dc.identifier.doi10.1002/cbic.201000340
dc.identifier.pmid20672284
dc.identifier.ppn346093260deu
dc.identifier.urihttp://kops.uni-konstanz.de/handle/123456789/13587
dc.language.isoengdeu
dc.legacy.dateIssued2011-06-17deu
dc.rightsterms-of-usedeu
dc.rights.urihttps://rightsstatements.org/page/InC/1.0/deu
dc.subjectCarbohydratesdeu
dc.subjectclick chemistrydeu
dc.subjectdendrimersdeu
dc.subjectinhibitorsdeu
dc.subjectmicroarraysdeu
dc.subject.ddc540deu
dc.titleRapid screening of lectins for multivalency effects with a glycodendrimer microarrayeng
dc.typeJOURNAL_ARTICLEdeu
dspace.entity.typePublication
kops.citation.bibtex
@article{Pera2010Rapid-13587,
  year={2010},
  doi={10.1002/cbic.201000340},
  title={Rapid screening of lectins for multivalency effects with a glycodendrimer microarray},
  number={13},
  volume={11},
  journal={ChemBioChem},
  pages={1896--1904},
  author={Pera, Núria Parera and Branderhorst, Hilbert M. and Kooij, Raymond and Maierhofer, Caroline and Liskamp, Rob M. J. and Wittmann, Valentin and Ruijtenbeek, Rob and Pieters, Roland J. and Kaaden, Marjolein van der}
}
kops.citation.iso690PERA, Núria Parera, Hilbert M. BRANDERHORST, Raymond KOOIJ, Caroline MAIERHOFER, Rob M. J. LISKAMP, Valentin WITTMANN, Rob RUIJTENBEEK, Roland J. PIETERS, Marjolein van der KAADEN, 2010. Rapid screening of lectins for multivalency effects with a glycodendrimer microarray. In: ChemBioChem. 2010, 11(13), pp. 1896-1904. eISSN 1439-4227. Available under: doi: 10.1002/cbic.201000340deu
kops.citation.iso690PERA, Núria Parera, Hilbert M. BRANDERHORST, Raymond KOOIJ, Caroline MAIERHOFER, Rob M. J. LISKAMP, Valentin WITTMANN, Rob RUIJTENBEEK, Roland J. PIETERS, Marjolein van der KAADEN, 2010. Rapid screening of lectins for multivalency effects with a glycodendrimer microarray. In: ChemBioChem. 2010, 11(13), pp. 1896-1904. eISSN 1439-4227. Available under: doi: 10.1002/cbic.201000340eng
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kops.sourcefieldChemBioChem. 2010, <b>11</b>(13), pp. 1896-1904. eISSN 1439-4227. Available under: doi: 10.1002/cbic.201000340deu
kops.sourcefield.plainChemBioChem. 2010, 11(13), pp. 1896-1904. eISSN 1439-4227. Available under: doi: 10.1002/cbic.201000340deu
kops.sourcefield.plainChemBioChem. 2010, 11(13), pp. 1896-1904. eISSN 1439-4227. Available under: doi: 10.1002/cbic.201000340eng
kops.submitter.emailoleg.kozlov@uni-konstanz.dedeu
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