Publikation:

Rapid screening of lectins for multivalency effects with a glycodendrimer microarray

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2010

Autor:innen

Pera, Núria Parera
Branderhorst, Hilbert M.
Kooij, Raymond
Maierhofer, Caroline
Liskamp, Rob M. J.
Ruijtenbeek, Rob
Pieters, Roland J.
Kaaden, Marjolein van der

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http://nbn-resolving.de/urn:nbn:de:bsz:352-135872
DOI (zitierfähiger Link)
https://doi.org/10.1002/cbic.201000340
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Open Access Green

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Chemie: Publikationen
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Published

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ChemBioChem. 2010, 11(13), pp. 1896-1904. eISSN 1439-4227. Available under: doi: 10.1002/cbic.201000340

Zusammenfassung

Multivalency is an important phenomenon in protein–carbohydrate interactions. In order to evaluate glycodendrimers as multivalent inhibitors of carbohydrate binding proteins, we displayed them on a microarray surface. Valencies were varied from 1 to 8, and corrections were made for the valencies so that all surfaces contained the same amount of the sugar ligand. Five different carbohydrates were attached to the dendrimers. A series of fluorescent lectins was evaluated, and for each of them a binding profile was obtained from a single experiment showing both the specificity of the lectin for a certain sugar and whether it prefers multivalent ligands or not. Very distinct binding patterns were seen for the various lectins. The results were rationalized with respect to the interbinding distances of the lectins.

Zusammenfassung in einer weiteren Sprache

Fachgebiet (DDC)
540 Chemie

Schlagwörter

Carbohydrates, click chemistry, dendrimers, inhibitors, microarrays

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ISO 690PERA, Núria Parera, Hilbert M. BRANDERHORST, Raymond KOOIJ, Caroline MAIERHOFER, Rob M. J. LISKAMP, Valentin WITTMANN, Rob RUIJTENBEEK, Roland J. PIETERS, Marjolein van der KAADEN, 2010. Rapid screening of lectins for multivalency effects with a glycodendrimer microarray. In: ChemBioChem. 2010, 11(13), pp. 1896-1904. eISSN 1439-4227. Available under: doi: 10.1002/cbic.201000340
BibTex
@article{Pera2010Rapid-13587,
  year={2010},
  doi={10.1002/cbic.201000340},
  title={Rapid screening of lectins for multivalency effects with a glycodendrimer microarray},
  number={13},
  volume={11},
  journal={ChemBioChem},
  pages={1896--1904},
  author={Pera, Núria Parera and Branderhorst, Hilbert M. and Kooij, Raymond and Maierhofer, Caroline and Liskamp, Rob M. J. and Wittmann, Valentin and Ruijtenbeek, Rob and Pieters, Roland J. and Kaaden, Marjolein van der}
}
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