Specific sequence selection and next generation resequencing of 68 E. coli genes using HybSelect

Kurzanzeige
dc.contributor.authorSchracke, Nadinedeu
dc.contributor.authorKornmeyer, Tobiasdeu
dc.contributor.authorKränzle, Marceldeu
dc.contributor.authorStähler, Peter F.deu
dc.contributor.authorSummerer, Daniel
dc.contributor.authorBeier, Markusdeu
dc.date.accessioned2011-08-19T12:52:57Zdeu
dc.date.available2011-08-19T12:52:57Zdeu
dc.date.issued2009-11-30
dc.description.abstractRecent years have seen tremendous progress in next generation sequencing technologies, allowing genomic sequencing in a highly cost-effective manner. However, sample preparation for these sequencers remains a bottleneck as the human genome is too complex to be routinely resequenced. We present here an in-depth study of HybSelect(TM), a method that can specifically enrich a large number of genes or regions of interest from any chromosomal DNA. The study used Escherichia coli K12 MG1655 as a model organism to test parameters such as method fidelity, capacity or reproducibility as a proof-of-principle.eng
dc.description.versionpublished
dc.identifier.citationPubl. in: New Biotechnology ; 26 (2009), 5. - pp. 229-233deu
dc.identifier.doi10.1016/j.nbt.2009.08.013deu
dc.identifier.pmid19735750
dc.identifier.urihttp://kops.uni-konstanz.de/handle/123456789/14965
dc.language.isoengdeu
dc.legacy.dateIssued2011-08-19deu
dc.rightsterms-of-usedeu
dc.rights.urihttps://rightsstatements.org/page/InC/1.0/deu
dc.subject.ddc570deu
dc.titleSpecific sequence selection and next generation resequencing of 68 E. coli genes using HybSelecteng
dc.typeJOURNAL_ARTICLEdeu
dspace.entity.typePublication
kops.citation.bibtex
@article{Schracke2009-11-30Speci-14965,
  year={2009},
  doi={10.1016/j.nbt.2009.08.013},
  title={Specific sequence selection and next generation resequencing of 68 E. coli genes using HybSelect},
  number={5},
  volume={26},
  issn={1871-6784},
  journal={New Biotechnology},
  pages={229--233},
  author={Schracke, Nadine and Kornmeyer, Tobias and Kränzle, Marcel and Stähler, Peter F. and Summerer, Daniel and Beier, Markus}
}
kops.citation.iso690SCHRACKE, Nadine, Tobias KORNMEYER, Marcel KRÄNZLE, Peter F. STÄHLER, Daniel SUMMERER, Markus BEIER, 2009. Specific sequence selection and next generation resequencing of 68 E. coli genes using HybSelect. In: New Biotechnology. 2009, 26(5), pp. 229-233. ISSN 1871-6784. eISSN 1876-4347. Available under: doi: 10.1016/j.nbt.2009.08.013deu
kops.citation.iso690SCHRACKE, Nadine, Tobias KORNMEYER, Marcel KRÄNZLE, Peter F. STÄHLER, Daniel SUMMERER, Markus BEIER, 2009. Specific sequence selection and next generation resequencing of 68 E. coli genes using HybSelect. In: New Biotechnology. 2009, 26(5), pp. 229-233. ISSN 1871-6784. eISSN 1876-4347. Available under: doi: 10.1016/j.nbt.2009.08.013eng
kops.citation.rdf
<rdf:RDF
    xmlns:dcterms="http://purl.org/dc/terms/"
    xmlns:dc="http://purl.org/dc/elements/1.1/"
    xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#"
    xmlns:bibo="http://purl.org/ontology/bibo/"
    xmlns:dspace="http://digital-repositories.org/ontologies/dspace/0.1.0#"
    xmlns:foaf="http://xmlns.com/foaf/0.1/"
    xmlns:void="http://rdfs.org/ns/void#"
    xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > 
  <rdf:Description rdf:about="https://kops.uni-konstanz.de/server/rdf/resource/123456789/14965">
    <dc:creator>Kränzle, Marcel</dc:creator>
    <dcterms:bibliographicCitation>Publ. in: New Biotechnology ; 26 (2009), 5. - pp. 229-233</dcterms:bibliographicCitation>
    <dcterms:isPartOf rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/52"/>
    <dc:rights>terms-of-use</dc:rights>
    <dc:language>eng</dc:language>
    <dcterms:rights rdf:resource="https://rightsstatements.org/page/InC/1.0/"/>
    <dc:creator>Kornmeyer, Tobias</dc:creator>
    <dc:creator>Beier, Markus</dc:creator>
    <void:sparqlEndpoint rdf:resource="http://localhost/fuseki/dspace/sparql"/>
    <dc:contributor>Beier, Markus</dc:contributor>
    <dcterms:title>Specific sequence selection and next generation resequencing of 68 E. coli genes using HybSelect</dcterms:title>
    <dc:creator>Schracke, Nadine</dc:creator>
    <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-08-19T12:52:57Z</dcterms:available>
    <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-08-19T12:52:57Z</dc:date>
    <dc:contributor>Summerer, Daniel</dc:contributor>
    <dc:creator>Summerer, Daniel</dc:creator>
    <dcterms:issued>2009-11-30</dcterms:issued>
    <bibo:uri rdf:resource="http://kops.uni-konstanz.de/handle/123456789/14965"/>
    <dspace:isPartOfCollection rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/52"/>
    <dc:contributor>Stähler, Peter F.</dc:contributor>
    <dc:contributor>Kränzle, Marcel</dc:contributor>
    <foaf:homepage rdf:resource="http://localhost:8080/"/>
    <dc:creator>Stähler, Peter F.</dc:creator>
    <dcterms:abstract xml:lang="eng">Recent years have seen tremendous progress in next generation sequencing technologies, allowing genomic sequencing in a highly cost-effective manner. However, sample preparation for these sequencers remains a bottleneck as the human genome is too complex to be routinely resequenced. We present here an in-depth study of HybSelect(TM), a method that can specifically enrich a large number of genes or regions of interest from any chromosomal DNA. The study used Escherichia coli K12 MG1655 as a model organism to test parameters such as method fidelity, capacity or reproducibility as a proof-of-principle.</dcterms:abstract>
    <dc:contributor>Kornmeyer, Tobias</dc:contributor>
    <dc:contributor>Schracke, Nadine</dc:contributor>
  </rdf:Description>
</rdf:RDF>
kops.flag.knbibliographyfalse
kops.identifier.nbnurn:nbn:de:bsz:352-149656deu
kops.sourcefieldNew Biotechnology. 2009, <b>26</b>(5), pp. 229-233. ISSN 1871-6784. eISSN 1876-4347. Available under: doi: 10.1016/j.nbt.2009.08.013deu
kops.sourcefield.plainNew Biotechnology. 2009, 26(5), pp. 229-233. ISSN 1871-6784. eISSN 1876-4347. Available under: doi: 10.1016/j.nbt.2009.08.013deu
kops.sourcefield.plainNew Biotechnology. 2009, 26(5), pp. 229-233. ISSN 1871-6784. eISSN 1876-4347. Available under: doi: 10.1016/j.nbt.2009.08.013eng
kops.submitter.emailkaren-ann.lindner@uni-konstanz.dedeu
relation.isAuthorOfPublication275bc4c2-db36-47f9-8e68-201bc5e7f911
relation.isAuthorOfPublication.latestForDiscovery275bc4c2-db36-47f9-8e68-201bc5e7f911
source.bibliographicInfo.fromPage229
source.bibliographicInfo.issue5
source.bibliographicInfo.toPage233
source.bibliographicInfo.volume26
source.identifier.eissn1876-4347
source.identifier.issn1871-6784
source.periodicalTitleNew Biotechnology

Dateien

Lizenzbündel

Gerade angezeigt 1 - 1 von 1
Vorschaubild nicht verfügbar
Name:
license.txt
Größe:
1.92 KB
Format:
Plain Text
Beschreibung:
license.txt
license.txtGröße: 1.92 KBDownloads: 0

Sammlungen

Zukunftskolleg: Publikationen