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Specific sequence selection and next generation resequencing of 68 E. coli genes using HybSelect

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2009

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Schracke, Nadine
Kornmeyer, Tobias
Kränzle, Marcel
Stähler, Peter F.
Beier, Markus

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http://nbn-resolving.de/urn:nbn:de:bsz:352-149656
DOI (zitierfähiger Link)
https://doi.org/10.1016/j.nbt.2009.08.013
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New Biotechnology. 2009, 26(5), pp. 229-233. ISSN 1871-6784. eISSN 1876-4347. Available under: doi: 10.1016/j.nbt.2009.08.013

Zusammenfassung

Recent years have seen tremendous progress in next generation sequencing technologies, allowing genomic sequencing in a highly cost-effective manner. However, sample preparation for these sequencers remains a bottleneck as the human genome is too complex to be routinely resequenced. We present here an in-depth study of HybSelect(TM), a method that can specifically enrich a large number of genes or regions of interest from any chromosomal DNA. The study used Escherichia coli K12 MG1655 as a model organism to test parameters such as method fidelity, capacity or reproducibility as a proof-of-principle.

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570 Biowissenschaften, Biologie

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ISO 690SCHRACKE, Nadine, Tobias KORNMEYER, Marcel KRÄNZLE, Peter F. STÄHLER, Daniel SUMMERER, Markus BEIER, 2009. Specific sequence selection and next generation resequencing of 68 E. coli genes using HybSelect. In: New Biotechnology. 2009, 26(5), pp. 229-233. ISSN 1871-6784. eISSN 1876-4347. Available under: doi: 10.1016/j.nbt.2009.08.013
BibTex
@article{Schracke2009-11-30Speci-14965,
  year={2009},
  doi={10.1016/j.nbt.2009.08.013},
  title={Specific sequence selection and next generation resequencing of 68 E. coli genes using HybSelect},
  number={5},
  volume={26},
  issn={1871-6784},
  journal={New Biotechnology},
  pages={229--233},
  author={Schracke, Nadine and Kornmeyer, Tobias and Kränzle, Marcel and Stähler, Peter F. and Summerer, Daniel and Beier, Markus}
}
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