Long-term Live Cell Microscopy Studies of Lipid Droplet Fusion Dynamics in Adipocytes

Abstract

During the adipogenic differentiation process of mesenchymal stem cells, lipid droplets (LDs) grow slowly by transferring lipids between each other. Recent findings hint at the possibility that a fusion pore is involved. In this study we analyze lipid transfer data obtained in long term label-free microscopy studies in the framework of a Hagen-Poiseuille model. The data obtained show a LD fusion process, in which the lipid transfer directionality depends on the size difference between LDs, whereas the respective rates depend on the size difference and additionally on the diameter of the smaller LD. For the data analysis, the viscosity of the transferred material has to be known. We demonstrate that a viscosity dependent molecular rotor dye can be used to measure LD viscosities in live cells. On this basis, we calculate the diameter of a putative lipid transfer channel which appears to have a direct dependence on the diameter of the smaller of the two participating LDs.