Publikation: Long-term Live Cell Microscopy Studies of Lipid Droplet Fusion Dynamics in Adipocytes
Lade...
Dateien
Zu diesem Dokument gibt es keine Dateien.
Datum
2013
Herausgeber:innen
ISSN der Zeitschrift
Electronic ISSN
ISBN
Bibliografische Daten
Verlag
Schriftenreihe
Auflagebezeichnung
URI (zitierfähiger Link)
DOI (zitierfähiger Link)
Internationale Patentnummer
Angaben zur Forschungsförderung
Projekt
Open Access-Veröffentlichung
Sammlungen
Core Facility der Universität Konstanz
Titel in einer weiteren Sprache
Publikationstyp
Zeitschriftenartikel
Publikationsstatus
Published
Erschienen in
The Journal of Lipid Research. 2013, 54(12), pp. 3419-3429. ISSN 0022-2275. eISSN 1539-7262. Available under: doi: 10.1194/jlr.M042515
Zusammenfassung
During the adipogenic differentiation process of mesenchymal stem cells, lipid droplets (LDs) grow slowly by transferring lipids between each other. Recent findings hint at the possibility that a fusion pore is involved. In this study we analyze lipid transfer data obtained in long term label-free microscopy studies in the framework of a Hagen-Poiseuille model. The data obtained show a LD fusion process, in which the lipid transfer directionality depends on the size difference between LDs, whereas the respective rates depend on the size difference and additionally on the diameter of the smaller LD. For the data analysis, the viscosity of the transferred material has to be known. We demonstrate that a viscosity dependent molecular rotor dye can be used to measure LD viscosities in live cells. On this basis, we calculate the diameter of a putative lipid transfer channel which appears to have a direct dependence on the diameter of the smaller of the two participating LDs.
Zusammenfassung in einer weiteren Sprache
Fachgebiet (DDC)
540 Chemie
Schlagwörter
Konferenz
Rezension
undefined / . - undefined, undefined
Zitieren
ISO 690
JÜNGST, Christian, Matthias KLEIN, Andreas ZUMBUSCH, 2013. Long-term Live Cell Microscopy Studies of Lipid Droplet Fusion Dynamics in Adipocytes. In: The Journal of Lipid Research. 2013, 54(12), pp. 3419-3429. ISSN 0022-2275. eISSN 1539-7262. Available under: doi: 10.1194/jlr.M042515BibTex
@article{Jungst2013-12Longt-24798,
year={2013},
doi={10.1194/jlr.M042515},
title={Long-term Live Cell Microscopy Studies of Lipid Droplet Fusion Dynamics in Adipocytes},
number={12},
volume={54},
issn={0022-2275},
journal={The Journal of Lipid Research},
pages={3419--3429},
author={Jüngst, Christian and Klein, Matthias and Zumbusch, Andreas}
}RDF
<rdf:RDF
xmlns:dcterms="http://purl.org/dc/terms/"
xmlns:dc="http://purl.org/dc/elements/1.1/"
xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#"
xmlns:bibo="http://purl.org/ontology/bibo/"
xmlns:dspace="http://digital-repositories.org/ontologies/dspace/0.1.0#"
xmlns:foaf="http://xmlns.com/foaf/0.1/"
xmlns:void="http://rdfs.org/ns/void#"
xmlns:xsd="http://www.w3.org/2001/XMLSchema#" >
<rdf:Description rdf:about="https://kops.uni-konstanz.de/server/rdf/resource/123456789/24798">
<dspace:isPartOfCollection rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/29"/>
<dcterms:rights rdf:resource="https://rightsstatements.org/page/InC/1.0/"/>
<dcterms:title>Long-term Live Cell Microscopy Studies of Lipid Droplet Fusion Dynamics in Adipocytes</dcterms:title>
<void:sparqlEndpoint rdf:resource="http://localhost/fuseki/dspace/sparql"/>
<dc:rights>terms-of-use</dc:rights>
<dc:contributor>Jüngst, Christian</dc:contributor>
<dc:creator>Klein, Matthias</dc:creator>
<dc:creator>Zumbusch, Andreas</dc:creator>
<dcterms:isPartOf rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/29"/>
<dcterms:abstract xml:lang="eng">During the adipogenic differentiation process of mesenchymal stem cells, lipid droplets (LDs) grow slowly by transferring lipids between each other. Recent findings hint at the possibility that a fusion pore is involved. In this study we analyze lipid transfer data obtained in long term label-free microscopy studies in the framework of a Hagen-Poiseuille model. The data obtained show a LD fusion process, in which the lipid transfer directionality depends on the size difference between LDs, whereas the respective rates depend on the size difference and additionally on the diameter of the smaller LD. For the data analysis, the viscosity of the transferred material has to be known. We demonstrate that a viscosity dependent molecular rotor dye can be used to measure LD viscosities in live cells. On this basis, we calculate the diameter of a putative lipid transfer channel which appears to have a direct dependence on the diameter of the smaller of the two participating LDs.</dcterms:abstract>
<dcterms:issued>2013-12</dcterms:issued>
<dc:contributor>Klein, Matthias</dc:contributor>
<foaf:homepage rdf:resource="http://localhost:8080/"/>
<dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2013-10-10T09:43:56Z</dc:date>
<dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2013-10-10T09:43:56Z</dcterms:available>
<dc:language>eng</dc:language>
<bibo:uri rdf:resource="http://kops.uni-konstanz.de/handle/123456789/24798"/>
<dcterms:bibliographicCitation>Journal of Lipid Research ; 24 (2013), 12. - S. 3419-3429</dcterms:bibliographicCitation>
<dc:contributor>Zumbusch, Andreas</dc:contributor>
<dc:creator>Jüngst, Christian</dc:creator>
</rdf:Description>
</rdf:RDF>Interner Vermerk
xmlui.Submission.submit.DescribeStep.inputForms.label.kops_note_fromSubmitter
Prüfungsdatum der Dissertation
Finanzierungsart
Kommentar zur Publikation
Allianzlizenz
Corresponding Authors der Uni Konstanz vorhanden
Internationale Co-Autor:innen
Universitätsbibliographie
Ja
