Role of Water in CaCO3 Biomineralization

Kurzanzeige
dc.contributor.authorLu, Hao
dc.contributor.authorHuang, Yu-Chieh
dc.contributor.authorHunger, Johannes
dc.contributor.authorGebauer, Denis
dc.contributor.authorCölfen, Helmut
dc.contributor.authorBonn, Mischa
dc.date.accessioned2021-03-23T08:21:05Z
dc.date.available2021-03-23T08:21:05Z
dc.date.issued2021-02-03eng
dc.description.abstractBiomineralization occurs in aqueous environments. Despite the ubiquity and relevance of CaCO3 biomineralization, the role of water in the biomineralization process has remained elusive. Here, we demonstrate that water reorganization accompanies CaCO3 biomineralization for sea urchin spine generation in a model system. Using surface-specific vibrational spectroscopy, we probe the water at the interface of the spine-associated protein during CaCO3 mineralization. Our results show that, while the protein structure remains unchanged, the structure of interfacial water is perturbed differently in the presence of both Ca2+ and CO32– compared to the addition of only Ca2+. This difference is attributed to the condensation of prenucleation mineral species. Our findings are consistent with a nonclassical mineralization pathway for sea urchin spine generation and highlight the importance of protein hydration in biomineralization.eng
dc.description.versionpublishedeng
dc.identifier.doi10.1021/jacs.0c11976eng
dc.identifier.pmid33471507eng
dc.identifier.ppn1752184793
dc.identifier.urihttps://kops.uni-konstanz.de/handle/123456789/53227
dc.language.isoengeng
dc.rightsAttribution 4.0 International
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subject.ddc540eng
dc.titleRole of Water in CaCO<sub>3</sub> Biomineralizationeng
dc.typeJOURNAL_ARTICLEeng
dspace.entity.typePublication
kops.citation.bibtex
@article{Lu2021-02-03Water-53227,
  year={2021},
  doi={10.1021/jacs.0c11976},
  title={Role of Water in CaCO<sub>3</sub> Biomineralization},
  number={4},
  volume={143},
  issn={0002-7863},
  journal={Journal of the American Chemical Society},
  pages={1758--1762},
  author={Lu, Hao and Huang, Yu-Chieh and Hunger, Johannes and Gebauer, Denis and Cölfen, Helmut and Bonn, Mischa}
}
kops.citation.iso690LU, Hao, Yu-Chieh HUANG, Johannes HUNGER, Denis GEBAUER, Helmut CÖLFEN, Mischa BONN, 2021. Role of Water in CaCO3 Biomineralization. In: Journal of the American Chemical Society. American Chemical Society. 2021, 143(4), pp. 1758-1762. ISSN 0002-7863. eISSN 1520-5126. Available under: doi: 10.1021/jacs.0c11976deu
kops.citation.iso690LU, Hao, Yu-Chieh HUANG, Johannes HUNGER, Denis GEBAUER, Helmut CÖLFEN, Mischa BONN, 2021. Role of Water in CaCO3 Biomineralization. In: Journal of the American Chemical Society. American Chemical Society. 2021, 143(4), pp. 1758-1762. ISSN 0002-7863. eISSN 1520-5126. Available under: doi: 10.1021/jacs.0c11976eng
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    <dcterms:abstract xml:lang="eng">Biomineralization occurs in aqueous environments. Despite the ubiquity and relevance of CaCO&lt;sub&gt;3&lt;/sub&gt; biomineralization, the role of water in the biomineralization process has remained elusive. Here, we demonstrate that water reorganization accompanies CaCO&lt;sub&gt;3&lt;/sub&gt; biomineralization for sea urchin spine generation in a model system. Using surface-specific vibrational spectroscopy, we probe the water at the interface of the spine-associated protein during CaCO&lt;sub&gt;3&lt;/sub&gt; mineralization. Our results show that, while the protein structure remains unchanged, the structure of interfacial water is perturbed differently in the presence of both Ca&lt;sup&gt;2+&lt;/sup&gt; and CO&lt;sub&gt;3&lt;/sub&gt;&lt;sup&gt;2–&lt;/sup&gt; compared to the addition of only Ca&lt;sup&gt;2+&lt;/sup&gt;. This difference is attributed to the condensation of prenucleation mineral species. Our findings are consistent with a nonclassical mineralization pathway for sea urchin spine generation and highlight the importance of protein hydration in biomineralization.</dcterms:abstract>
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kops.sourcefield.plainJournal of the American Chemical Society. American Chemical Society. 2021, 143(4), pp. 1758-1762. ISSN 0002-7863. eISSN 1520-5126. Available under: doi: 10.1021/jacs.0c11976eng
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