Role of Water in CaCO3 Biomineralization

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2021
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Lu, Hao
Hunger, Johannes
Bonn, Mischa
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Journal of the American Chemical Society. American Chemical Society. 2021, 143(4), pp. 1758-1762. ISSN 0002-7863. eISSN 1520-5126. Available under: doi: 10.1021/jacs.0c11976
Zusammenfassung

Biomineralization occurs in aqueous environments. Despite the ubiquity and relevance of CaCO3 biomineralization, the role of water in the biomineralization process has remained elusive. Here, we demonstrate that water reorganization accompanies CaCO3 biomineralization for sea urchin spine generation in a model system. Using surface-specific vibrational spectroscopy, we probe the water at the interface of the spine-associated protein during CaCO3 mineralization. Our results show that, while the protein structure remains unchanged, the structure of interfacial water is perturbed differently in the presence of both Ca2+ and CO32– compared to the addition of only Ca2+. This difference is attributed to the condensation of prenucleation mineral species. Our findings are consistent with a nonclassical mineralization pathway for sea urchin spine generation and highlight the importance of protein hydration in biomineralization.

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ISO 690LU, Hao, Yu-Chieh HUANG, Johannes HUNGER, Denis GEBAUER, Helmut CÖLFEN, Mischa BONN, 2021. Role of Water in CaCO3 Biomineralization. In: Journal of the American Chemical Society. American Chemical Society. 2021, 143(4), pp. 1758-1762. ISSN 0002-7863. eISSN 1520-5126. Available under: doi: 10.1021/jacs.0c11976
BibTex
@article{Lu2021-02-03Water-53227,
  year={2021},
  doi={10.1021/jacs.0c11976},
  title={Role of Water in CaCO<sub>3</sub> Biomineralization},
  number={4},
  volume={143},
  issn={0002-7863},
  journal={Journal of the American Chemical Society},
  pages={1758--1762},
  author={Lu, Hao and Huang, Yu-Chieh and Hunger, Johannes and Gebauer, Denis and Cölfen, Helmut and Bonn, Mischa}
}
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