Crystallization and preliminary X-ray analysis of the tungsten-dependent acetylene hydratase from Pelobacter acetylenicus

Kurzanzeige
dc.contributor.authorEinsle, Oliverdeu
dc.contributor.authorNiessen, Holgerdeu
dc.contributor.authorAbt, Dietmar J.deu
dc.contributor.authorSeiffert, Grażyna Bernadetadeu
dc.contributor.authorSchink, Bernhard
dc.contributor.authorHuber, Robertdeu
dc.contributor.authorMesserschmidt, Albrechtdeu
dc.contributor.authorKroneck, Peter M. H.
dc.date.accessioned2011-03-24T17:39:41Zdeu
dc.date.available2011-03-24T17:39:41Zdeu
dc.date.issued2005deu
dc.description.abstractAcetylene hydratase is a tungsten-containing hydroxylase that converts acetylene to acetaldehyde in a unique reaction that requires a strong reductant. The subsequent disproportionation of acetaldehyde yields acetate and ethanol. Crystals of the tungsten/iron-sulfur protein acetylene hydratase from Pelobacter acetylenicus strain WoAcy 1 (DSM 3246) were grown by the vapour-diffusion method in an N2/H2 atmosphere using polyethylene glycol as precipitant.Growth of crystas suitable for X-ray analysis strictly depended on the presence of TiIII citrate or dithionite as reducing agents.deu
dc.description.versionpublished
dc.format.mimetypeapplication/pdfdeu
dc.identifier.citationFirst publ. in: Acta Crystallographica Sect. F61, 2005, pp. 299-301deu
dc.identifier.doi10.1107/S174430910500374X
dc.identifier.pmid16511024
dc.identifier.ppn262007797deu
dc.identifier.urihttp://kops.uni-konstanz.de/handle/123456789/8067
dc.language.isoengdeu
dc.legacy.dateIssued2007deu
dc.rightsterms-of-usedeu
dc.rights.urihttps://rightsstatements.org/page/InC/1.0/deu
dc.subject.ddc570deu
dc.titleCrystallization and preliminary X-ray analysis of the tungsten-dependent acetylene hydratase from Pelobacter acetylenicuseng
dc.typeJOURNAL_ARTICLEdeu
dspace.entity.typePublication
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@article{Einsle2005Cryst-8067,
  year={2005},
  doi={10.1107/S174430910500374X},
  title={Crystallization and preliminary X-ray analysis of the tungsten-dependent acetylene hydratase from Pelobacter acetylenicus},
  number={3},
  volume={1},
  journal={Acta Crystallographica Sect  F6},
  pages={299--301},
  author={Einsle, Oliver and Niessen, Holger and Abt, Dietmar J. and Seiffert, Grażyna Bernadeta and Schink, Bernhard and Huber, Robert and Messerschmidt, Albrecht and Kroneck, Peter M. H.}
}
kops.citation.iso690EINSLE, Oliver, Holger NIESSEN, Dietmar J. ABT, Grażyna Bernadeta SEIFFERT, Bernhard SCHINK, Robert HUBER, Albrecht MESSERSCHMIDT, Peter M. H. KRONECK, 2005. Crystallization and preliminary X-ray analysis of the tungsten-dependent acetylene hydratase from Pelobacter acetylenicus. In: Acta Crystallographica Sect F6. 2005, 1(3), pp. 299-301. eISSN 1744-3091. Available under: doi: 10.1107/S174430910500374Xdeu
kops.citation.iso690EINSLE, Oliver, Holger NIESSEN, Dietmar J. ABT, Grażyna Bernadeta SEIFFERT, Bernhard SCHINK, Robert HUBER, Albrecht MESSERSCHMIDT, Peter M. H. KRONECK, 2005. Crystallization and preliminary X-ray analysis of the tungsten-dependent acetylene hydratase from Pelobacter acetylenicus. In: Acta Crystallographica Sect F6. 2005, 1(3), pp. 299-301. eISSN 1744-3091. Available under: doi: 10.1107/S174430910500374Xeng
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