Publikation: Crystallization and preliminary X-ray analysis of the tungsten-dependent acetylene hydratase from Pelobacter acetylenicus
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2005
Autor:innen
Einsle, Oliver
Niessen, Holger
Abt, Dietmar J.
Seiffert, Grażyna Bernadeta
Huber, Robert
Messerschmidt, Albrecht
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Acta Crystallographica Sect F6. 2005, 1(3), pp. 299-301. eISSN 1744-3091. Available under: doi: 10.1107/S174430910500374X
Zusammenfassung
Acetylene hydratase is a tungsten-containing hydroxylase that converts acetylene to acetaldehyde in a unique reaction that requires a strong reductant. The subsequent disproportionation of acetaldehyde yields acetate and ethanol. Crystals of the tungsten/iron-sulfur protein acetylene hydratase from Pelobacter acetylenicus strain WoAcy 1 (DSM 3246) were grown by the vapour-diffusion method in an N2/H2 atmosphere using polyethylene glycol as precipitant.Growth of crystas suitable for X-ray analysis strictly depended on the presence of TiIII citrate or dithionite as reducing agents.
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570 Biowissenschaften, Biologie
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EINSLE, Oliver, Holger NIESSEN, Dietmar J. ABT, Grażyna Bernadeta SEIFFERT, Bernhard SCHINK, Robert HUBER, Albrecht MESSERSCHMIDT, Peter M. H. KRONECK, 2005. Crystallization and preliminary X-ray analysis of the tungsten-dependent acetylene hydratase from Pelobacter acetylenicus. In: Acta Crystallographica Sect F6. 2005, 1(3), pp. 299-301. eISSN 1744-3091. Available under: doi: 10.1107/S174430910500374XBibTex
@article{Einsle2005Cryst-8067,
year={2005},
doi={10.1107/S174430910500374X},
title={Crystallization and preliminary X-ray analysis of the tungsten-dependent acetylene hydratase from Pelobacter acetylenicus},
number={3},
volume={1},
journal={Acta Crystallographica Sect F6},
pages={299--301},
author={Einsle, Oliver and Niessen, Holger and Abt, Dietmar J. and Seiffert, Grażyna Bernadeta and Schink, Bernhard and Huber, Robert and Messerschmidt, Albrecht and Kroneck, Peter M. H.}
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<dcterms:abstract xml:lang="deu">Acetylene hydratase is a tungsten-containing hydroxylase that converts acetylene to acetaldehyde in a unique reaction that requires a strong reductant. The subsequent disproportionation of acetaldehyde yields acetate and ethanol. Crystals of the tungsten/iron-sulfur protein acetylene hydratase from Pelobacter acetylenicus strain WoAcy 1 (DSM 3246) were grown by the vapour-diffusion method in an N2/H2 atmosphere using polyethylene glycol as precipitant.Growth of crystas suitable for X-ray analysis strictly depended on the presence of TiIII citrate or dithionite as reducing agents.</dcterms:abstract>
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