Crystallization and preliminary X-ray analysis of the tungsten-dependent acetylene hydratase from Pelobacter acetylenicus

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Einsle_ActaCryst_F61_299.pdf
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2005
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Einsle, Oliver
Niessen, Holger
Abt, Dietmar J.
Seiffert, Grażyna Bernadeta
Huber, Robert
Messerschmidt, Albrecht
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Acetylene hydratase is a tungsten-containing hydroxylase that converts acetylene to acetaldehyde in a unique reaction that requires a strong reductant. The subsequent disproportionation of acetaldehyde yields acetate and ethanol. Crystals of the tungsten/iron-sulfur protein acetylene hydratase from Pelobacter acetylenicus strain WoAcy 1 (DSM 3246) were grown by the vapour-diffusion method in an N2/H2 atmosphere using polyethylene glycol as precipitant.Growth of crystas suitable for X-ray analysis strictly depended on the presence of TiIII citrate or dithionite as reducing agents.

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ISO 690EINSLE, Oliver, Holger NIESSEN, Dietmar J. ABT, Grażyna Bernadeta SEIFFERT, Bernhard SCHINK, Robert HUBER, Albrecht MESSERSCHMIDT, Peter M. H. KRONECK, 2005. Crystallization and preliminary X-ray analysis of the tungsten-dependent acetylene hydratase from Pelobacter acetylenicus. In: Acta Crystallographica Sect F6. 2005, 1(3), pp. 299-301. eISSN 1744-3091. Available under: doi: 10.1107/S174430910500374X
BibTex
@article{Einsle2005Cryst-8067,
  year={2005},
  doi={10.1107/S174430910500374X},
  title={Crystallization and preliminary X-ray analysis of the tungsten-dependent acetylene hydratase from Pelobacter acetylenicus},
  number={3},
  volume={1},
  journal={Acta Crystallographica Sect  F6},
  pages={299--301},
  author={Einsle, Oliver and Niessen, Holger and Abt, Dietmar J. and Seiffert, Grażyna Bernadeta and Schink, Bernhard and Huber, Robert and Messerschmidt, Albrecht and Kroneck, Peter M. H.}
}
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