Crystallization and preliminary X-ray analysis of the tungsten-dependent acetylene hydratase from Pelobacter acetylenicus
Crystallization and preliminary X-ray analysis of the tungsten-dependent acetylene hydratase from Pelobacter acetylenicus
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2005
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Einsle, Oliver
Niessen, Holger
Abt, Dietmar J.
Seiffert, Grażyna Bernadeta
Huber, Robert
Messerschmidt, Albrecht
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Acta Crystallographica Sect F6 ; 1 (2005), 3. - S. 299-301. - eISSN 1744-3091
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Acetylene hydratase is a tungsten-containing hydroxylase that converts acetylene to acetaldehyde in a unique reaction that requires a strong reductant. The subsequent disproportionation of acetaldehyde yields acetate and ethanol. Crystals of the tungsten/iron-sulfur protein acetylene hydratase from Pelobacter acetylenicus strain WoAcy 1 (DSM 3246) were grown by the vapour-diffusion method in an N2/H2 atmosphere using polyethylene glycol as precipitant.Growth of crystas suitable for X-ray analysis strictly depended on the presence of TiIII citrate or dithionite as reducing agents.
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EINSLE, Oliver, Holger NIESSEN, Dietmar J. ABT, Grażyna Bernadeta SEIFFERT, Bernhard SCHINK, Robert HUBER, Albrecht MESSERSCHMIDT, Peter M. H. KRONECK, 2005. Crystallization and preliminary X-ray analysis of the tungsten-dependent acetylene hydratase from Pelobacter acetylenicus. In: Acta Crystallographica Sect F6. 1(3), pp. 299-301. eISSN 1744-3091. Available under: doi: 10.1107/S174430910500374XBibTex
@article{Einsle2005Cryst-8067, year={2005}, doi={10.1107/S174430910500374X}, title={Crystallization and preliminary X-ray analysis of the tungsten-dependent acetylene hydratase from Pelobacter acetylenicus}, number={3}, volume={1}, journal={Acta Crystallographica Sect F6}, pages={299--301}, author={Einsle, Oliver and Niessen, Holger and Abt, Dietmar J. and Seiffert, Grażyna Bernadeta and Schink, Bernhard and Huber, Robert and Messerschmidt, Albrecht and Kroneck, Peter M. H.} }
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