Structural Basis for the KlenTaq DNA Polymerase Catalysed Incorporation of Alkene- versus Alkyne-Modified Nucleotides

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dc.contributor.authorHottin, Audrey
dc.contributor.authorBetz, Karin
dc.contributor.authorDiederichs, Kay
dc.contributor.authorMarx, Andreas
dc.date.accessioned2017-04-03T10:51:34Z
dc.date.available2017-04-03T10:51:34Z
dc.date.issued2017-02-10eng
dc.description.abstractEfficient incorporation of modified nucleotides by DNA polymerases is essential for many cutting-edge biomolecular technologies. The present study compares the acceptance of either alkene- or alkyne-modified nucleotides by KlenTaq DNA polymerase and provides structural insights into how 7-deaza-adenosine and deoxyuridine with attached alkene-modifications are incorporated into the growing DNA strand. Thereby, we identified modified nucleotides that prove to be superior substrates for KlenTaq DNA polymerase compared with their natural analogues. The knowledge can be used to guide future design of functionalized nucleotide building blocks.eng
dc.description.versionpublishedeng
dc.identifier.doi10.1002/chem.201604515eng
dc.identifier.pmid27901305eng
dc.identifier.ppn489127223
dc.identifier.urihttps://kops.uni-konstanz.de/handle/123456789/38270
dc.language.isoengeng
dc.rightsterms-of-use
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dc.subject.ddc570eng
dc.titleStructural Basis for the KlenTaq DNA Polymerase Catalysed Incorporation of Alkene- versus Alkyne-Modified Nucleotideseng
dc.typeJOURNAL_ARTICLEeng
dspace.entity.typePublication
kops.citation.bibtex
@article{Hottin2017-02-10Struc-38270,
  year={2017},
  doi={10.1002/chem.201604515},
  title={Structural Basis for the KlenTaq DNA Polymerase Catalysed Incorporation of Alkene- versus Alkyne-Modified Nucleotides},
  number={9},
  volume={23},
  issn={0947-6539},
  journal={Chemistry - A European Journal},
  pages={2109--2118},
  author={Hottin, Audrey and Betz, Karin and Diederichs, Kay and Marx, Andreas}
}
kops.citation.iso690HOTTIN, Audrey, Karin BETZ, Kay DIEDERICHS, Andreas MARX, 2017. Structural Basis for the KlenTaq DNA Polymerase Catalysed Incorporation of Alkene- versus Alkyne-Modified Nucleotides. In: Chemistry - A European Journal. 2017, 23(9), pp. 2109-2118. ISSN 0947-6539. eISSN 1521-3765. Available under: doi: 10.1002/chem.201604515deu
kops.citation.iso690HOTTIN, Audrey, Karin BETZ, Kay DIEDERICHS, Andreas MARX, 2017. Structural Basis for the KlenTaq DNA Polymerase Catalysed Incorporation of Alkene- versus Alkyne-Modified Nucleotides. In: Chemistry - A European Journal. 2017, 23(9), pp. 2109-2118. ISSN 0947-6539. eISSN 1521-3765. Available under: doi: 10.1002/chem.201604515eng
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