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Structural Basis for the KlenTaq DNA Polymerase Catalysed Incorporation of Alkene- versus Alkyne-Modified Nucleotides

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2017

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http://nbn-resolving.de/urn:nbn:de:bsz:352-0-398415
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https://doi.org/10.1002/chem.201604515
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Chemistry - A European Journal. 2017, 23(9), pp. 2109-2118. ISSN 0947-6539. eISSN 1521-3765. Available under: doi: 10.1002/chem.201604515

Zusammenfassung

Efficient incorporation of modified nucleotides by DNA polymerases is essential for many cutting-edge biomolecular technologies. The present study compares the acceptance of either alkene- or alkyne-modified nucleotides by KlenTaq DNA polymerase and provides structural insights into how 7-deaza-adenosine and deoxyuridine with attached alkene-modifications are incorporated into the growing DNA strand. Thereby, we identified modified nucleotides that prove to be superior substrates for KlenTaq DNA polymerase compared with their natural analogues. The knowledge can be used to guide future design of functionalized nucleotide building blocks.

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570 Biowissenschaften, Biologie

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ISO 690HOTTIN, Audrey, Karin BETZ, Kay DIEDERICHS, Andreas MARX, 2017. Structural Basis for the KlenTaq DNA Polymerase Catalysed Incorporation of Alkene- versus Alkyne-Modified Nucleotides. In: Chemistry - A European Journal. 2017, 23(9), pp. 2109-2118. ISSN 0947-6539. eISSN 1521-3765. Available under: doi: 10.1002/chem.201604515
BibTex
@article{Hottin2017-02-10Struc-38270,
  year={2017},
  doi={10.1002/chem.201604515},
  title={Structural Basis for the KlenTaq DNA Polymerase Catalysed Incorporation of Alkene- versus Alkyne-Modified Nucleotides},
  number={9},
  volume={23},
  issn={0947-6539},
  journal={Chemistry - A European Journal},
  pages={2109--2118},
  author={Hottin, Audrey and Betz, Karin and Diederichs, Kay and Marx, Andreas}
}
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