Non-proteolytic ubiquitylation counteracts the APC/C-inhibitory function of XErp1

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dc.contributor.authorHörmanseder, Eva Beate
dc.contributor.authorTischer, Thomas
dc.contributor.authorHeubes, Simonedeu
dc.contributor.authorStemmann, Olafdeu
dc.contributor.authorMayer, Thomas U.
dc.date.accessioned2011-06-17T08:03:06Zdeu
dc.date.available2011-06-17T08:03:06Zdeu
dc.date.issued2011-05
dc.description.abstractMature Xenopus oocytes are arrested in meiosis by the activity of XErp1/Emi2, an inhibitor of the ubiquitin-ligase anaphase-promoting complex/cyclosome (APC/C). On fertilization, XErp1 is degraded, resulting in APC/C activation and the consequent degradation of cell-cycle regulators and exit from meiosis. In this study, we show that a modest increase in the activity of the ubiquitin-conjugating enzyme UbcX overrides the meiotic arrest in an APC/C-dependent reaction. Intriguingly, XErp1 remains stable in these conditions. We found that UbcX causes the ubiquitylation of XErp1, followed by its dissociation from the APC/C. Our data support the idea that ubiquitylation regulates the APC/C-inhibitory activity of XErp1.eng
dc.description.versionpublished
dc.identifier.citationPubl. in: EMBO reports 12 (2011), 5, pp. 436-443deu
dc.identifier.doi10.1038/embor.2011.32deu
dc.identifier.pmid21399619
dc.identifier.ppn415519942
dc.identifier.urihttp://kops.uni-konstanz.de/handle/123456789/13685
dc.language.isoengdeu
dc.legacy.dateIssued2011-06-17deu
dc.rightsterms-of-usedeu
dc.rights.urihttps://rightsstatements.org/page/InC/1.0/deu
dc.subject.ddc570deu
dc.titleNon-proteolytic ubiquitylation counteracts the APC/C-inhibitory function of XErp1eng
dc.typeJOURNAL_ARTICLEdeu
dspace.entity.typePublication
kops.citation.bibtex
@article{Hormanseder2011-05Nonpr-13685,
  year={2011},
  doi={10.1038/embor.2011.32},
  title={Non-proteolytic ubiquitylation counteracts the APC/C-inhibitory function of XErp1},
  number={5},
  volume={12},
  issn={1469-221X},
  journal={EMBO reports},
  pages={436--443},
  author={Hörmanseder, Eva Beate and Tischer, Thomas and Heubes, Simone and Stemmann, Olaf and Mayer, Thomas U.}
}
kops.citation.iso690HÖRMANSEDER, Eva Beate, Thomas TISCHER, Simone HEUBES, Olaf STEMMANN, Thomas U. MAYER, 2011. Non-proteolytic ubiquitylation counteracts the APC/C-inhibitory function of XErp1. In: EMBO reports. 2011, 12(5), pp. 436-443. ISSN 1469-221X. eISSN 1469-3178. Available under: doi: 10.1038/embor.2011.32deu
kops.citation.iso690HÖRMANSEDER, Eva Beate, Thomas TISCHER, Simone HEUBES, Olaf STEMMANN, Thomas U. MAYER, 2011. Non-proteolytic ubiquitylation counteracts the APC/C-inhibitory function of XErp1. In: EMBO reports. 2011, 12(5), pp. 436-443. ISSN 1469-221X. eISSN 1469-3178. Available under: doi: 10.1038/embor.2011.32eng
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kops.relation.uniknProjectTitleSP Mechanisms of Genome Haploidization -Regulatory Mechanisms of Meiosis in Vertebrate Oocytes-
kops.sourcefieldEMBO reports. 2011, <b>12</b>(5), pp. 436-443. ISSN 1469-221X. eISSN 1469-3178. Available under: doi: 10.1038/embor.2011.32deu
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kops.sourcefield.plainEMBO reports. 2011, 12(5), pp. 436-443. ISSN 1469-221X. eISSN 1469-3178. Available under: doi: 10.1038/embor.2011.32eng
kops.submitter.emailthomas.u.mayer@uni-konstanz.dedeu
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