Non-proteolytic ubiquitylation counteracts the APC/C-inhibitory function of XErp1

Hörmanseder, Eva Beate; Tischer, Thomas; Heubes, Simone; Stemmann, Olaf; Mayer, Thomas U.

Non-proteolytic ubiquitylation counteracts the APC/C-inhibitory function of XErp1

Dateien

Hoermanseder_136853.pdf(703.84 KB)

Datum

2011

Autor:innen

Hörmanseder, Eva Beate

Tischer, Thomas

Heubes, Simone

Stemmann, Olaf

Mayer, Thomas U.

Projekt

SP Mechanisms of Genome Haploidization -Regulatory Mechanisms of Meiosis in Vertebrate Oocytes-

Sammlungen

Biologie

Publikationstyp

Zeitschriftenartikel

Erschienen in

EMBO reports ; 12 (2011), 5. - S. 436-443. - ISSN 1469-221X. - eISSN 1469-3178

Zusammenfassung

Mature Xenopus oocytes are arrested in meiosis by the activity of XErp1/Emi2, an inhibitor of the ubiquitin-ligase anaphase-promoting complex/cyclosome (APC/C). On fertilization, XErp1 is degraded, resulting in APC/C activation and the consequent degradation of cell-cycle regulators and exit from meiosis. In this study, we show that a modest increase in the activity of the ubiquitin-conjugating enzyme UbcX overrides the meiotic arrest in an APC/C-dependent reaction. Intriguingly, XErp1 remains stable in these conditions. We found that UbcX causes the ubiquitylation of XErp1, followed by its dissociation from the APC/C. Our data support the idea that ubiquitylation regulates the APC/C-inhibitory activity of XErp1.

Fachgebiet (DDC)

570 Biowissenschaften, Biologie

Zitieren

ISO 690

HÖRMANSEDER, Eva Beate, Thomas TISCHER, Simone HEUBES, Olaf STEMMANN, Thomas U. MAYER, 2011. Non-proteolytic ubiquitylation counteracts the APC/C-inhibitory function of XErp1. In: EMBO reports. 12(5), pp. 436-443. ISSN 1469-221X. eISSN 1469-3178. Available under: doi: 10.1038/embor.2011.32

BibTex

@article{Hormanseder2011-05Nonpr-13685,
  year={2011},
  doi={10.1038/embor.2011.32},
  title={Non-proteolytic ubiquitylation counteracts the APC/C-inhibitory function of XErp1},
  number={5},
  volume={12},
  issn={1469-221X},
  journal={EMBO reports},
  pages={436--443},
  author={Hörmanseder, Eva Beate and Tischer, Thomas and Heubes, Simone and Stemmann, Olaf and Mayer, Thomas U.}
}

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Universitätsbibliographie

Ja