Adsorption of ß-Lactoglobulin on spherical polyelectrolyte brushes : Direct proof of counterion release by isothermal titration calorimetry

Kurzanzeige
dc.contributor.authorHenzler, Katjadeu
dc.contributor.authorHaupt, Björndeu
dc.contributor.authorLauterbach, Karlheinzdeu
dc.contributor.authorWittemann, Alexander
dc.contributor.authorBorisov, Olegdeu
dc.contributor.authorBallauff, Matthiasdeu
dc.date.accessioned2012-08-22T07:41:26Zdeu
dc.date.available2012-08-22T07:41:26Zdeu
dc.date.issued2010-03-10
dc.description.abstractThe thermodynamics and the driving forces of the adsorption of ß-lactoglobulin on spherical polyelectrolyte brushes (SPB) are investigated by isothermal titration calorimetry (ITC). The SPB consist of a polystyrene core onto which long chains of poly(styrene sulfonate) are grafted. Adsorption isothermsare obtained from measurements by ITC. The analysis by ITC shows clearly that the adsorption process is solely driven by entropy while ΔH > 0. This finding is in accordance with the proposed mechanism of counterion release: Patches of positive charges on the surface of the proteins become multivalent counterions of the polyelectrolyte chains, thereby releasing the counterions of the protein and the polyelectrolyte. A simple statistical-mechanical model fully corroborates the proposed mechanism. The present analysis shows clearly the fundamental importance of counterion release for protein adsorption on charged interfaces and charged polymeric layers.deu
dc.description.versionpublished
dc.identifier.citationPubl. in: Journal of the American Chemical Society ; 132 (2010), 9. - pp. 3159–3163deu
dc.identifier.doi10.1021/ja909938cdeu
dc.identifier.pmid20143809
dc.identifier.urihttp://kops.uni-konstanz.de/handle/123456789/20184
dc.language.isodeudeu
dc.legacy.dateIssued2012-08-22deu
dc.rightsterms-of-usedeu
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dc.subject.ddc540deu
dc.titleAdsorption of ß-Lactoglobulin on spherical polyelectrolyte brushes : Direct proof of counterion release by isothermal titration calorimetrydeu
dc.typeJOURNAL_ARTICLEdeu
dspace.entity.typePublication
kops.citation.bibtex
@article{Henzler2010-03-10Adsor-20184,
  year={2010},
  doi={10.1021/ja909938c},
  title={Adsorption of ß-Lactoglobulin on spherical polyelectrolyte brushes : Direct proof of counterion release by isothermal titration calorimetry},
  number={9},
  volume={132},
  issn={0002-7863},
  journal={Journal of the American Chemical Society},
  pages={3159--3163},
  author={Henzler, Katja and Haupt, Björn and Lauterbach, Karlheinz and Wittemann, Alexander and Borisov, Oleg and Ballauff, Matthias}
}
kops.citation.iso690HENZLER, Katja, Björn HAUPT, Karlheinz LAUTERBACH, Alexander WITTEMANN, Oleg BORISOV, Matthias BALLAUFF, 2010. Adsorption of ß-Lactoglobulin on spherical polyelectrolyte brushes : Direct proof of counterion release by isothermal titration calorimetry. In: Journal of the American Chemical Society. 2010, 132(9), pp. 3159-3163. ISSN 0002-7863. eISSN 1520-5126. Available under: doi: 10.1021/ja909938cdeu
kops.citation.iso690HENZLER, Katja, Björn HAUPT, Karlheinz LAUTERBACH, Alexander WITTEMANN, Oleg BORISOV, Matthias BALLAUFF, 2010. Adsorption of ß-Lactoglobulin on spherical polyelectrolyte brushes : Direct proof of counterion release by isothermal titration calorimetry. In: Journal of the American Chemical Society. 2010, 132(9), pp. 3159-3163. ISSN 0002-7863. eISSN 1520-5126. Available under: doi: 10.1021/ja909938ceng
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kops.sourcefield.plainJournal of the American Chemical Society. 2010, 132(9), pp. 3159-3163. ISSN 0002-7863. eISSN 1520-5126. Available under: doi: 10.1021/ja909938ceng
kops.submitter.emailregina.fleischmann@uni-konstanz.dedeu
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