Adsorption of ß-Lactoglobulin on spherical polyelectrolyte brushes : Direct proof of counterion release by isothermal titration calorimetry

Henzler, Katja; Haupt, Björn; Lauterbach, Karlheinz; Wittemann, Alexander; Borisov, Oleg; Ballauff, Matthias

Adsorption of ß-Lactoglobulin on spherical polyelectrolyte brushes : Direct proof of counterion release by isothermal titration calorimetry

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Date

2010

Authors

Henzler, Katja

Haupt, Björn

Lauterbach, Karlheinz

Wittemann, Alexander

Borisov, Oleg

Ballauff, Matthias

Publication type

Journal article

Published in

Journal of the American Chemical Society ; 132 (2010), 9. - pp. 3159-3163. - ISSN 0002-7863. - eISSN 1520-5126

Abstract

The thermodynamics and the driving forces of the adsorption of ß-lactoglobulin on spherical polyelectrolyte brushes (SPB) are investigated by isothermal titration calorimetry (ITC). The SPB consist of a polystyrene core onto which long chains of poly(styrene sulfonate) are grafted. Adsorption isothermsare obtained from measurements by ITC. The analysis by ITC shows clearly that the adsorption process is solely driven by entropy while ΔH > 0. This finding is in accordance with the proposed mechanism of counterion release: Patches of positive charges on the surface of the proteins become multivalent counterions of the polyelectrolyte chains, thereby releasing the counterions of the protein and the polyelectrolyte. A simple statistical-mechanical model fully corroborates the proposed mechanism. The present analysis shows clearly the fundamental importance of counterion release for protein adsorption on charged interfaces and charged polymeric layers.

Subject (DDC)

540 Chemistry

Cite This

ISO 690

HENZLER, Katja, Björn HAUPT, Karlheinz LAUTERBACH, Alexander WITTEMANN, Oleg BORISOV, Matthias BALLAUFF, 2010. Adsorption of ß-Lactoglobulin on spherical polyelectrolyte brushes : Direct proof of counterion release by isothermal titration calorimetry. In: Journal of the American Chemical Society. 132(9), pp. 3159-3163. ISSN 0002-7863. eISSN 1520-5126. Available under: doi: 10.1021/ja909938c

BibTex

@article{Henzler2010-03-10Adsor-20184,
  year={2010},
  doi={10.1021/ja909938c},
  title={Adsorption of ß-Lactoglobulin on spherical polyelectrolyte brushes : Direct proof of counterion release by isothermal titration calorimetry},
  number={9},
  volume={132},
  issn={0002-7863},
  journal={Journal of the American Chemical Society},
  pages={3159--3163},
  author={Henzler, Katja and Haupt, Björn and Lauterbach, Karlheinz and Wittemann, Alexander and Borisov, Oleg and Ballauff, Matthias}
}

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