Adsorption of ß-Lactoglobulin on spherical polyelectrolyte brushes : Direct proof of counterion release by isothermal titration calorimetry

Lade...
Vorschaubild
Dateien
Zu diesem Dokument gibt es keine Dateien.
Datum
2010
Autor:innen
Henzler, Katja
Haupt, Björn
Lauterbach, Karlheinz
Borisov, Oleg
Ballauff, Matthias
Herausgeber:innen
Kontakt
ISSN der Zeitschrift
Electronic ISSN
ISBN
Bibliografische Daten
Verlag
Schriftenreihe
Auflagebezeichnung
DOI (zitierfähiger Link)
ArXiv-ID
Internationale Patentnummer
Angaben zur Forschungsförderung
Projekt
Open Access-Veröffentlichung
Sammlungen
Core Facility der Universität Konstanz
Gesperrt bis
Titel in einer weiteren Sprache
Forschungsvorhaben
Organisationseinheiten
Zeitschriftenheft
Publikationstyp
Zeitschriftenartikel
Publikationsstatus
Published
Erschienen in
Zusammenfassung

The thermodynamics and the driving forces of the adsorption of ß-lactoglobulin on spherical polyelectrolyte brushes (SPB) are investigated by isothermal titration calorimetry (ITC). The SPB consist of a polystyrene core onto which long chains of poly(styrene sulfonate) are grafted. Adsorption isothermsare obtained from measurements by ITC. The analysis by ITC shows clearly that the adsorption process is solely driven by entropy while ΔH > 0. This finding is in accordance with the proposed mechanism of counterion release: Patches of positive charges on the surface of the proteins become multivalent counterions of the polyelectrolyte chains, thereby releasing the counterions of the protein and the polyelectrolyte. A simple statistical-mechanical model fully corroborates the proposed mechanism. The present analysis shows clearly the fundamental importance of counterion release for protein adsorption on charged interfaces and charged polymeric layers.

Zusammenfassung in einer weiteren Sprache
Fachgebiet (DDC)
540 Chemie
Schlagwörter
Konferenz
Rezension
undefined / . - undefined, undefined
Zitieren
ISO 690HENZLER, Katja, Björn HAUPT, Karlheinz LAUTERBACH, Alexander WITTEMANN, Oleg BORISOV, Matthias BALLAUFF, 2010. Adsorption of ß-Lactoglobulin on spherical polyelectrolyte brushes : Direct proof of counterion release by isothermal titration calorimetry. In: Journal of the American Chemical Society. 2010, 132(9), pp. 3159-3163. ISSN 0002-7863. eISSN 1520-5126. Available under: doi: 10.1021/ja909938c
BibTex
@article{Henzler2010-03-10Adsor-20184,
  year={2010},
  doi={10.1021/ja909938c},
  title={Adsorption of ß-Lactoglobulin on spherical polyelectrolyte brushes : Direct proof of counterion release by isothermal titration calorimetry},
  number={9},
  volume={132},
  issn={0002-7863},
  journal={Journal of the American Chemical Society},
  pages={3159--3163},
  author={Henzler, Katja and Haupt, Björn and Lauterbach, Karlheinz and Wittemann, Alexander and Borisov, Oleg and Ballauff, Matthias}
}
RDF
<rdf:RDF
    xmlns:dcterms="http://purl.org/dc/terms/"
    xmlns:dc="http://purl.org/dc/elements/1.1/"
    xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#"
    xmlns:bibo="http://purl.org/ontology/bibo/"
    xmlns:dspace="http://digital-repositories.org/ontologies/dspace/0.1.0#"
    xmlns:foaf="http://xmlns.com/foaf/0.1/"
    xmlns:void="http://rdfs.org/ns/void#"
    xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > 
  <rdf:Description rdf:about="https://kops.uni-konstanz.de/server/rdf/resource/123456789/20184">
    <dc:contributor>Henzler, Katja</dc:contributor>
    <dcterms:issued>2010-03-10</dcterms:issued>
    <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2012-08-22T07:41:26Z</dcterms:available>
    <dc:contributor>Wittemann, Alexander</dc:contributor>
    <dc:contributor>Ballauff, Matthias</dc:contributor>
    <foaf:homepage rdf:resource="http://localhost:8080/"/>
    <dc:creator>Haupt, Björn</dc:creator>
    <dc:contributor>Borisov, Oleg</dc:contributor>
    <dc:creator>Ballauff, Matthias</dc:creator>
    <dc:creator>Lauterbach, Karlheinz</dc:creator>
    <dcterms:bibliographicCitation>Publ. in: Journal of the American Chemical Society ; 132 (2010), 9. - pp. 3159–3163</dcterms:bibliographicCitation>
    <dcterms:title>Adsorption of ß-Lactoglobulin on spherical polyelectrolyte brushes : Direct proof of counterion release by isothermal titration calorimetry</dcterms:title>
    <void:sparqlEndpoint rdf:resource="http://localhost/fuseki/dspace/sparql"/>
    <dcterms:abstract xml:lang="deu">The thermodynamics and the driving forces of the adsorption of ß-lactoglobulin on spherical polyelectrolyte brushes (SPB) are investigated by isothermal titration calorimetry (ITC). The SPB consist of a polystyrene core onto which long chains of poly(styrene sulfonate) are grafted. Adsorption isothermsare obtained from measurements by ITC. The analysis by  ITC shows clearly that the adsorption process is solely driven by entropy while ΔH &gt; 0. This finding is in accordance with the proposed mechanism of counterion release: Patches of positive charges on the surface of the proteins become multivalent counterions of the polyelectrolyte chains, thereby releasing the counterions of the protein and the polyelectrolyte. A simple statistical-mechanical model fully corroborates the proposed mechanism. The present analysis shows clearly the fundamental importance of counterion release for protein adsorption on charged interfaces and charged polymeric layers.</dcterms:abstract>
    <dcterms:isPartOf rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/29"/>
    <dspace:isPartOfCollection rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/29"/>
    <dc:creator>Wittemann, Alexander</dc:creator>
    <dc:creator>Borisov, Oleg</dc:creator>
    <dcterms:rights rdf:resource="https://rightsstatements.org/page/InC/1.0/"/>
    <dc:creator>Henzler, Katja</dc:creator>
    <bibo:uri rdf:resource="http://kops.uni-konstanz.de/handle/123456789/20184"/>
    <dc:contributor>Haupt, Björn</dc:contributor>
    <dc:contributor>Lauterbach, Karlheinz</dc:contributor>
    <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2012-08-22T07:41:26Z</dc:date>
    <dc:rights>terms-of-use</dc:rights>
    <dc:language>deu</dc:language>
  </rdf:Description>
</rdf:RDF>
Interner Vermerk
xmlui.Submission.submit.DescribeStep.inputForms.label.kops_note_fromSubmitter
Kontakt
URL der Originalveröffentl.
Prüfdatum der URL
Prüfungsdatum der Dissertation
Finanzierungsart
Kommentar zur Publikation
Allianzlizenz
Corresponding Authors der Uni Konstanz vorhanden
Internationale Co-Autor:innen
Universitätsbibliographie
Nein
Begutachtet
Diese Publikation teilen