A molecular beacon for quantitative monitoring of the DNA polymerase reaction in real-time

Kurzanzeige
dc.contributor.authorSummerer, Daniel
dc.contributor.authorMarx, Andreas
dc.date.accessioned2011-06-29T14:27:36Zdeu
dc.date.available2011-06-29T14:27:36Zdeu
dc.date.issued2002
dc.description.abstractAn assay for real-time monitoring of DNA synthesis by DNA polymerases is presented. With the opening of a dual dye-labeled probe, triggered by the progressing enzymatic DNA synthesis, a fluorescent signal is generated (see scheme), enabling the high-throughput quantitative characterization of inhibitors and monitoring of the DNA polymerase reaction in cell lysates.eng
dc.description.versionpublished
dc.format.mimetypeapplication/pdfdeu
dc.identifier.citationFirst publ. in: Angewandte Chemie International Edition 41 (2002), 19, pp. 3620–3622deu
dc.identifier.doi10.1002/1521-3773(20021004)41:19<3620::AID-ANIE3620>3.0.CO;2-Cdeu
dc.identifier.ppn346729793deu
dc.identifier.urihttp://kops.uni-konstanz.de/handle/123456789/361
dc.language.isoengdeu
dc.legacy.dateIssued2011deu
dc.rightsterms-of-usedeu
dc.rights.urihttps://rightsstatements.org/page/InC/1.0/deu
dc.subject.ddc540deu
dc.subject.gndDNA polymerasesdeu
dc.subject.gndenzyme assaydeu
dc.subject.gndenzyme inhibitorsdeu
dc.subject.gndhigh-throughput screeningdeu
dc.subject.gndreporter systemsdeu
dc.titleA molecular beacon for quantitative monitoring of the DNA polymerase reaction in real-timeeng
dc.typeJOURNAL_ARTICLEdeu
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  year={2002},
  doi={10.1002/1521-3773(20021004)41:19<3620::AID-ANIE3620>3.0.CO;2-C},
  title={A molecular beacon for quantitative monitoring of the DNA polymerase reaction in real-time},
  number={19},
  volume={41},
  issn={1433-7851},
  journal={Angewandte Chemie International Edition},
  pages={3620--3622},
  author={Summerer, Daniel and Marx, Andreas}
}
kops.citation.iso690SUMMERER, Daniel, Andreas MARX, 2002. A molecular beacon for quantitative monitoring of the DNA polymerase reaction in real-time. In: Angewandte Chemie International Edition. 2002, 41(19), pp. 3620-3622. ISSN 1433-7851. Available under: doi: 10.1002/1521-3773(20021004)41:19<3620::AID-ANIE3620>3.0.CO;2-Cdeu
kops.citation.iso690SUMMERER, Daniel, Andreas MARX, 2002. A molecular beacon for quantitative monitoring of the DNA polymerase reaction in real-time. In: Angewandte Chemie International Edition. 2002, 41(19), pp. 3620-3622. ISSN 1433-7851. Available under: doi: 10.1002/1521-3773(20021004)41:19<3620::AID-ANIE3620>3.0.CO;2-Ceng
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