Publikation:

A molecular beacon for quantitative monitoring of the DNA polymerase reaction in real-time

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2002

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Angewandte Chemie International Edition. 2002, 41(19), pp. 3620-3622. ISSN 1433-7851. Available under: doi: 10.1002/1521-3773(20021004)41:19<3620::AID-ANIE3620>3.0.CO;2-C

Zusammenfassung

An assay for real-time monitoring of DNA synthesis by DNA polymerases is presented. With the opening of a dual dye-labeled probe, triggered by the progressing enzymatic DNA synthesis, a fluorescent signal is generated (see scheme), enabling the high-throughput quantitative characterization of inhibitors and monitoring of the DNA polymerase reaction in cell lysates.

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540 Chemie

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ISO 690SUMMERER, Daniel, Andreas MARX, 2002. A molecular beacon for quantitative monitoring of the DNA polymerase reaction in real-time. In: Angewandte Chemie International Edition. 2002, 41(19), pp. 3620-3622. ISSN 1433-7851. Available under: doi: 10.1002/1521-3773(20021004)41:19<3620::AID-ANIE3620>3.0.CO;2-C
BibTex
@article{Summerer2002molec-361,
  year={2002},
  doi={10.1002/1521-3773(20021004)41:19<3620::AID-ANIE3620>3.0.CO;2-C},
  title={A molecular beacon for quantitative monitoring of the DNA polymerase reaction in real-time},
  number={19},
  volume={41},
  issn={1433-7851},
  journal={Angewandte Chemie International Edition},
  pages={3620--3622},
  author={Summerer, Daniel and Marx, Andreas}
}
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