Publikation: A molecular beacon for quantitative monitoring of the DNA polymerase reaction in real-time
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2002
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Angewandte Chemie International Edition. 2002, 41(19), pp. 3620-3622. ISSN 1433-7851. Available under: doi: 10.1002/1521-3773(20021004)41:19<3620::AID-ANIE3620>3.0.CO;2-C
Zusammenfassung
An assay for real-time monitoring of DNA synthesis by DNA polymerases is presented. With the opening of a dual dye-labeled probe, triggered by the progressing enzymatic DNA synthesis, a fluorescent signal is generated (see scheme), enabling the high-throughput quantitative characterization of inhibitors and monitoring of the DNA polymerase reaction in cell lysates.
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540 Chemie
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SUMMERER, Daniel, Andreas MARX, 2002. A molecular beacon for quantitative monitoring of the DNA polymerase reaction in real-time. In: Angewandte Chemie International Edition. 2002, 41(19), pp. 3620-3622. ISSN 1433-7851. Available under: doi: 10.1002/1521-3773(20021004)41:19<3620::AID-ANIE3620>3.0.CO;2-CBibTex
@article{Summerer2002molec-361, year={2002}, doi={10.1002/1521-3773(20021004)41:19<3620::AID-ANIE3620>3.0.CO;2-C}, title={A molecular beacon for quantitative monitoring of the DNA polymerase reaction in real-time}, number={19}, volume={41}, issn={1433-7851}, journal={Angewandte Chemie International Edition}, pages={3620--3622}, author={Summerer, Daniel and Marx, Andreas} }
RDF
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