Using protease inhibitors in antigen presentation assays

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dc.contributor.authorBasler, Michael
dc.contributor.authorGroettrup, Marcus
dc.date.accessioned2013-02-26T11:10:04Zdeu
dc.date.available2013-02-26T11:10:04Zdeu
dc.date.issued2013
dc.description.abstractThe major histocompatibility complex (MHC) class I restricted pathway of antigen processing allows the presentation of intracellular antigens to cytotoxic T lymphocytes. The proteasome is the main protease in the cytoplasm and the nucleus, which is responsible for the generation of most peptide ligands of MHC-I molecules. Peptides produced by the proteasome can be further trimmed or destroyed by numerous cytosolic or endoplasmic reticulum (ER) lumenal proteases. Small molecule inhibitors are useful tools for probing the role of proteases in MHC class I antigen processing. Here, we describe different methods to test the impact of protease inhibitors in antigen presentation assays.eng
dc.description.versionpublished
dc.identifier.citationAntigen Processing : Methods and Protocols / ed. by Peter van Endert. - Totowa, NJ : Humana Press, 2012. - S. 31-39. - (Methods in Molecular Biology ; 960). - ISBN 978-1-627-03217-9deu
dc.identifier.doi10.1007/978-1-62703-218-6_3deu
dc.identifier.pmid23329476
dc.identifier.urihttp://kops.uni-konstanz.de/handle/123456789/21985
dc.language.isoengdeu
dc.legacy.dateIssued2013-02-26deu
dc.rightsterms-of-usedeu
dc.rights.urihttps://rightsstatements.org/page/InC/1.0/deu
dc.subjectProteasedeu
dc.subjectProteasomedeu
dc.subjectInhibitondeu
dc.subjectAntigen presentationdeu
dc.subjectAntigen processingdeu
dc.subjectMHC-I surface stainingdeu
dc.subjectlacZ assaydeu
dc.subjectT cell hybridomasdeu
dc.subjectIntracellular cytokine stainingdeu
dc.subjectT cell linesdeu
dc.subject.ddc570deu
dc.titleUsing protease inhibitors in antigen presentation assayseng
dc.typeINCOLLECTIONdeu
dspace.entity.typePublication
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  doi={10.1007/978-1-62703-218-6_3},
  title={Using protease inhibitors in antigen presentation assays},
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  publisher={Humana Press},
  address={Totowa, NJ},
  series={Methods in Molecular Biology},
  booktitle={Antigen Processing},
  pages={31--39},
  editor={van Endert, Peter},
  author={Basler, Michael and Gröttrup, Marcus}
}
kops.citation.iso690BASLER, Michael, Marcus GRÖTTRUP, 2013. Using protease inhibitors in antigen presentation assays. In: VAN ENDERT, Peter, ed.. Antigen Processing. Totowa, NJ: Humana Press, 2013, pp. 31-39. Methods in Molecular Biology. 960. ISBN 978-1-62703-217-9. Available under: doi: 10.1007/978-1-62703-218-6_3deu
kops.citation.iso690BASLER, Michael, Marcus GRÖTTRUP, 2013. Using protease inhibitors in antigen presentation assays. In: VAN ENDERT, Peter, ed.. Antigen Processing. Totowa, NJ: Humana Press, 2013, pp. 31-39. Methods in Molecular Biology. 960. ISBN 978-1-62703-217-9. Available under: doi: 10.1007/978-1-62703-218-6_3eng
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kops.sourcefieldVAN ENDERT, Peter, ed.. <i>Antigen Processing</i>. Totowa, NJ: Humana Press, 2013, pp. 31-39. Methods in Molecular Biology. 960. ISBN 978-1-62703-217-9. Available under: doi: 10.1007/978-1-62703-218-6_3deu
kops.sourcefield.plainVAN ENDERT, Peter, ed.. Antigen Processing. Totowa, NJ: Humana Press, 2013, pp. 31-39. Methods in Molecular Biology. 960. ISBN 978-1-62703-217-9. Available under: doi: 10.1007/978-1-62703-218-6_3deu
kops.sourcefield.plainVAN ENDERT, Peter, ed.. Antigen Processing. Totowa, NJ: Humana Press, 2013, pp. 31-39. Methods in Molecular Biology. 960. ISBN 978-1-62703-217-9. Available under: doi: 10.1007/978-1-62703-218-6_3eng
kops.submitter.emailbrigitte.schanze@uni-konstanz.dedeu
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source.relation.ispartofseriesMethods in Molecular Biology
source.titleAntigen Processing

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