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Using protease inhibitors in antigen presentation assays

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2013

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http://nbn-resolving.de/urn:nbn:de:bsz:352-219857
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https://doi.org/10.1007/978-1-62703-218-6_3
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VAN ENDERT, Peter, ed.. Antigen Processing. Totowa, NJ: Humana Press, 2013, pp. 31-39. Methods in Molecular Biology. 960. ISBN 978-1-62703-217-9. Available under: doi: 10.1007/978-1-62703-218-6_3

Zusammenfassung

The major histocompatibility complex (MHC) class I restricted pathway of antigen processing allows the presentation of intracellular antigens to cytotoxic T lymphocytes. The proteasome is the main protease in the cytoplasm and the nucleus, which is responsible for the generation of most peptide ligands of MHC-I molecules. Peptides produced by the proteasome can be further trimmed or destroyed by numerous cytosolic or endoplasmic reticulum (ER) lumenal proteases. Small molecule inhibitors are useful tools for probing the role of proteases in MHC class I antigen processing. Here, we describe different methods to test the impact of protease inhibitors in antigen presentation assays.

Zusammenfassung in einer weiteren Sprache

Fachgebiet (DDC)
570 Biowissenschaften, Biologie

Schlagwörter

Protease, Proteasome, Inhibiton, Antigen presentation, Antigen processing, MHC-I surface staining, lacZ assay, T cell hybridomas, Intracellular cytokine staining, T cell lines

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ISO 690BASLER, Michael, Marcus GRÖTTRUP, 2013. Using protease inhibitors in antigen presentation assays. In: VAN ENDERT, Peter, ed.. Antigen Processing. Totowa, NJ: Humana Press, 2013, pp. 31-39. Methods in Molecular Biology. 960. ISBN 978-1-62703-217-9. Available under: doi: 10.1007/978-1-62703-218-6_3
BibTex
@incollection{Basler2013Using-21985,
  year={2013},
  doi={10.1007/978-1-62703-218-6_3},
  title={Using protease inhibitors in antigen presentation assays},
  number={960},
  isbn={978-1-62703-217-9},
  publisher={Humana Press},
  address={Totowa, NJ},
  series={Methods in Molecular Biology},
  booktitle={Antigen Processing},
  pages={31--39},
  editor={van Endert, Peter},
  author={Basler, Michael and Gröttrup, Marcus}
}
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