Encapsulation of proteins and peptides into biodegradable poly(D,L-lactide-co-glycolide) microspheres prolongs and enhances antigen presentation by human dendritic cells

Kurzanzeige
dc.contributor.authorWaeckerle-Men, Yingdeu
dc.contributor.authorUetz-von Allmen, Edith
dc.contributor.authorGander, Brunodeu
dc.contributor.authorScandella, Elkedeu
dc.contributor.authorSchlosser, Eva
dc.contributor.authorSchmidtke, Gunter
dc.contributor.authorMerkle, Hans P.deu
dc.contributor.authorGroettrup, Marcus
dc.date.accessioned2013-03-06T11:06:14Zdeu
dc.date.available2013-03-06T11:06:14Zdeu
dc.date.issued2006-03-10
dc.description.abstractDendritic cell (DC)-based immunotherapy has been hampered by the lack of suitable methods for antigen delivery. Here, we use biodegradable poly(d,l-lactide-co-glycolide) microspheres (PLGA-MS) as carriers of peptides and proteins for antigen delivery to human monocyte-derived DC (MoDC). Compared to soluble proteins, MHC classes I and II-restricted presentation of PLGA-MS-encapsulated proteins and peptides by MoDC was markedly prolonged and proteins were presented 50-fold more efficiently on class I molecules. The vaccination of mice with DC loaded with PLGA-MS-encapsulated proteins raised strong and persisting cytotoxic T cell responses. In conclusion, antigen delivery via PLGA-MS markedly enhanced the duration of antigen presentation by human MoDC and the potency of DC-based vaccination.eng
dc.description.versionpublished
dc.identifier.citationVaccine ; 24 (2006), 11. - S. 1847-1857deu
dc.identifier.doi10.1016/j.vaccine.2005.10.032deu
dc.identifier.pmid16288821
dc.identifier.ppn497078066
dc.identifier.urihttp://kops.uni-konstanz.de/handle/123456789/22117
dc.language.isoengdeu
dc.legacy.dateIssued2013-03-06deu
dc.rightsterms-of-usedeu
dc.rights.urihttps://rightsstatements.org/page/InC/1.0/deu
dc.subjectPLGA microspheresdeu
dc.subjectDendritic cellsdeu
dc.subjectAntigen deliverydeu
dc.subjectAntigen presentationdeu
dc.subject.ddc570deu
dc.titleEncapsulation of proteins and peptides into biodegradable poly(D,L-lactide-co-glycolide) microspheres prolongs and enhances antigen presentation by human dendritic cellseng
dc.typeJOURNAL_ARTICLEdeu
dspace.entity.typePublication
kops.citation.bibtex
@article{WaeckerleMen2006-03-10Encap-22117,
  year={2006},
  doi={10.1016/j.vaccine.2005.10.032},
  title={Encapsulation of proteins and peptides into biodegradable poly(D,L-lactide-co-glycolide) microspheres prolongs and enhances antigen presentation by human dendritic cells},
  number={11},
  volume={24},
  issn={0264-410X},
  journal={Vaccine},
  pages={1847--1857},
  author={Waeckerle-Men, Ying and Uetz-von Allmen, Edith and Gander, Bruno and Scandella, Elke and Schlosser, Eva and Schmidtke, Gunter and Merkle, Hans P. and Gröttrup, Marcus}
}
kops.citation.iso690WAECKERLE-MEN, Ying, Edith UETZ-VON ALLMEN, Bruno GANDER, Elke SCANDELLA, Eva SCHLOSSER, Gunter SCHMIDTKE, Hans P. MERKLE, Marcus GRÖTTRUP, 2006. Encapsulation of proteins and peptides into biodegradable poly(D,L-lactide-co-glycolide) microspheres prolongs and enhances antigen presentation by human dendritic cells. In: Vaccine. 2006, 24(11), pp. 1847-1857. ISSN 0264-410X. eISSN 1873-2518. Available under: doi: 10.1016/j.vaccine.2005.10.032deu
kops.citation.iso690WAECKERLE-MEN, Ying, Edith UETZ-VON ALLMEN, Bruno GANDER, Elke SCANDELLA, Eva SCHLOSSER, Gunter SCHMIDTKE, Hans P. MERKLE, Marcus GRÖTTRUP, 2006. Encapsulation of proteins and peptides into biodegradable poly(D,L-lactide-co-glycolide) microspheres prolongs and enhances antigen presentation by human dendritic cells. In: Vaccine. 2006, 24(11), pp. 1847-1857. ISSN 0264-410X. eISSN 1873-2518. Available under: doi: 10.1016/j.vaccine.2005.10.032eng
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kops.sourcefieldVaccine. 2006, <b>24</b>(11), pp. 1847-1857. ISSN 0264-410X. eISSN 1873-2518. Available under: doi: 10.1016/j.vaccine.2005.10.032deu
kops.sourcefield.plainVaccine. 2006, 24(11), pp. 1847-1857. ISSN 0264-410X. eISSN 1873-2518. Available under: doi: 10.1016/j.vaccine.2005.10.032deu
kops.sourcefield.plainVaccine. 2006, 24(11), pp. 1847-1857. ISSN 0264-410X. eISSN 1873-2518. Available under: doi: 10.1016/j.vaccine.2005.10.032eng
kops.submitter.emailbrigitte.schanze@uni-konstanz.dedeu
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source.periodicalTitleVaccine

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