Simultaneous IR-spectroscopic observation of α-synuclein, lipids, and solvent reveals an alternative membrane-induced oligomerization pathway

Kurzanzeige
dc.contributor.authorFallah, Mohammad A.
dc.contributor.authorGerding, Hanne R.
dc.contributor.authorScheibe, Christian
dc.contributor.authorDrescher, Malte
dc.contributor.authorKarreman, Christiaan
dc.contributor.authorSchildknecht, Stefan
dc.contributor.authorLeist, Marcel
dc.contributor.authorHauser, Karin
dc.date.accessioned2017-10-13T11:26:09Z
dc.date.available2017-10-13T11:26:09Z
dc.date.issued2017-12-05
dc.description.abstractThe intrinsically disordered protein α-synuclein (αS), a known pathogenic factor for Parkinson's disease, can adopt defined secondary structures when interacting with membranes or during fibrillation. The αS-lipid interaction and the implications of this process for aggregation and damage to membranes are still poorly understood. Therefore, we established a label-free infrared (IR) spectroscopic approach to simultaneously monitor αS conformation and membrane integrity. IR showed its unique sensitivity for identifying distinct α-structured aggregates. A comparative study of wildtype αS and the naturally occurring splicing variant αS Δexon3 yielded new insights into the membrane's capability of altering aggregation pathways.eng
dc.description.versionpublishedeng
dc.identifier.doi10.1002/cbic.201700355eng
dc.identifier.pmid28980756eng
dc.identifier.ppn497823535
dc.identifier.urihttps://kops.uni-konstanz.de/handle/123456789/40331
dc.language.isoengeng
dc.rightsterms-of-use
dc.rights.urihttps://rightsstatements.org/page/InC/1.0/
dc.subject.ddc570eng
dc.titleSimultaneous IR-spectroscopic observation of α-synuclein, lipids, and solvent reveals an alternative membrane-induced oligomerization pathwayeng
dc.typeJOURNAL_ARTICLEeng
dspace.entity.typePublication
kops.citation.bibtex
@article{Fallah2017-12-05Simul-40331,
  year={2017},
  doi={10.1002/cbic.201700355},
  title={Simultaneous IR-spectroscopic observation of α-synuclein, lipids, and solvent reveals an alternative membrane-induced oligomerization pathway},
  number={23},
  volume={18},
  issn={1439-4227},
  journal={ChemBioChem},
  pages={2312--2316},
  author={Fallah, Mohammad A. and Gerding, Hanne R. and Scheibe, Christian and Drescher, Malte and Karreman, Christiaan and Schildknecht, Stefan and Leist, Marcel and Hauser, Karin}
}
kops.citation.iso690FALLAH, Mohammad A., Hanne R. GERDING, Christian SCHEIBE, Malte DRESCHER, Christiaan KARREMAN, Stefan SCHILDKNECHT, Marcel LEIST, Karin HAUSER, 2017. Simultaneous IR-spectroscopic observation of α-synuclein, lipids, and solvent reveals an alternative membrane-induced oligomerization pathway. In: ChemBioChem. 2017, 18(23), pp. 2312-2316. ISSN 1439-4227. eISSN 1439-7633. Available under: doi: 10.1002/cbic.201700355deu
kops.citation.iso690FALLAH, Mohammad A., Hanne R. GERDING, Christian SCHEIBE, Malte DRESCHER, Christiaan KARREMAN, Stefan SCHILDKNECHT, Marcel LEIST, Karin HAUSER, 2017. Simultaneous IR-spectroscopic observation of α-synuclein, lipids, and solvent reveals an alternative membrane-induced oligomerization pathway. In: ChemBioChem. 2017, 18(23), pp. 2312-2316. ISSN 1439-4227. eISSN 1439-7633. Available under: doi: 10.1002/cbic.201700355eng
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