Improved LNA probe-based assay for the detection of African and South American yellow fever virus strains

Kurzanzeige
dc.contributor.authorWeidmann, Manfreddeu
dc.contributor.authorFaye, Ousmanedeu
dc.contributor.authorFaye, Oumardeu
dc.contributor.authorKranaster, Ramon
dc.contributor.authorMarx, Andreas
dc.contributor.authorNunes, Marcio R. T.deu
dc.contributor.authorVasconcelos, Pedro F. C.deu
dc.contributor.authorHufert, Frank T.deu
dc.contributor.authorSall, Amadou A.deu
dc.date.accessioned2011-06-16T08:49:09Zdeu
dc.date.available2011-06-16T08:49:09Zdeu
dc.date.issued2010deu
dc.description.abstractBackground
Real-time assays for Yellow fever virus (YFV) would help to improve acute diagnostics in outbreak investigations.

Objectives
To develop a real-time assay for YFV able to detect African and South American strains.

Study design
Three short probe (14–18 nt) formats were compared and a plasmid-transcribed RNA standard was used to test the performance of the assays. Additionally the new TaqM1 enzyme was tested.

Results
A locked nucleotide probe (LNA probe) performed best with an analytical sensitivity of 10 RNA molecules detected. 44 African and 10 South American strains were detectable. One South American strain from 1984 had a one-nucleotide deviation in the hybridisation sequence for which the LNA probe had to be adapted. Comparison of enzymes revealed that not all enzymes are suitable for LNA probes.

Conclusion
The developed LNA probe based YFV real-time PCR performed best in an enzyme mix and less efficient using multifunctional enzymes.		eng
dc.description.versionpublished
dc.identifier.citationFirst publ. in: Journal of Clinical Virology 48 (2010), 3, pp. 187-192deu
dc.identifier.doi10.1016/j.jcv.2010.04.013
dc.identifier.pmid20556888
dc.identifier.ppn469396326
dc.identifier.urihttp://kops.uni-konstanz.de/handle/123456789/12601
dc.language.isoengdeu
dc.legacy.dateIssued2011-06-16deu
dc.rightsterms-of-usedeu
dc.rights.urihttps://rightsstatements.org/page/InC/1.0/deu
dc.subjectYFVdeu
dc.subjectReal-time PCRdeu
dc.subjectLNA probedeu
dc.subjectMGB probedeu
dc.subjectEclipse probedeu
dc.subjectTaqM1 enzymedeu
dc.subject.ddc540deu
dc.titleImproved LNA probe-based assay for the detection of African and South American yellow fever virus strainseng
dc.typeJOURNAL_ARTICLEdeu
dspace.entity.typePublication
kops.citation.bibtex
@article{Weidmann2010Impro-12601,
  year={2010},
  doi={10.1016/j.jcv.2010.04.013},
  title={Improved LNA probe-based assay for the detection of African and South American yellow fever virus strains},
  number={3},
  volume={48},
  issn={1386-6532},
  journal={Journal of Clinical Virology},
  pages={187--192},
  author={Weidmann, Manfred and Faye, Ousmane and Faye, Oumar and Kranaster, Ramon and Marx, Andreas and Nunes, Marcio R. T. and Vasconcelos, Pedro F. C. and Hufert, Frank T. and Sall, Amadou A.}
}
kops.citation.iso690WEIDMANN, Manfred, Ousmane FAYE, Oumar FAYE, Ramon KRANASTER, Andreas MARX, Marcio R. T. NUNES, Pedro F. C. VASCONCELOS, Frank T. HUFERT, Amadou A. SALL, 2010. Improved LNA probe-based assay for the detection of African and South American yellow fever virus strains. In: Journal of Clinical Virology. 2010, 48(3), pp. 187-192. ISSN 1386-6532. eISSN 1873-5967. Available under: doi: 10.1016/j.jcv.2010.04.013deu
kops.citation.iso690WEIDMANN, Manfred, Ousmane FAYE, Oumar FAYE, Ramon KRANASTER, Andreas MARX, Marcio R. T. NUNES, Pedro F. C. VASCONCELOS, Frank T. HUFERT, Amadou A. SALL, 2010. Improved LNA probe-based assay for the detection of African and South American yellow fever virus strains. In: Journal of Clinical Virology. 2010, 48(3), pp. 187-192. ISSN 1386-6532. eISSN 1873-5967. Available under: doi: 10.1016/j.jcv.2010.04.013eng
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kops.sourcefieldJournal of Clinical Virology. 2010, <b>48</b>(3), pp. 187-192. ISSN 1386-6532. eISSN 1873-5967. Available under: doi: 10.1016/j.jcv.2010.04.013deu
kops.sourcefield.plainJournal of Clinical Virology. 2010, 48(3), pp. 187-192. ISSN 1386-6532. eISSN 1873-5967. Available under: doi: 10.1016/j.jcv.2010.04.013deu
kops.sourcefield.plainJournal of Clinical Virology. 2010, 48(3), pp. 187-192. ISSN 1386-6532. eISSN 1873-5967. Available under: doi: 10.1016/j.jcv.2010.04.013eng
kops.submitter.emailmichael.ketzer@uni-konstanz.dedeu
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