Publikation:

Improved LNA probe-based assay for the detection of African and South American yellow fever virus strains

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Weidmann_126015.pdf
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Datum

2010

Autor:innen

Weidmann, Manfred
Faye, Ousmane
Faye, Oumar
Nunes, Marcio R. T.
Vasconcelos, Pedro F. C.
Hufert, Frank T.
Sall, Amadou A.

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URI (zitierfähiger Link)
http://nbn-resolving.de/urn:nbn:de:bsz:352-126015
DOI (zitierfähiger Link)
https://doi.org/10.1016/j.jcv.2010.04.013
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Urheberrechtlich geschützt

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Open Access-Veröffentlichung
Open Access Green

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Chemie: Publikationen
Core Facility der Universität Konstanz

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Titel in einer weiteren Sprache

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Published

Erschienen in

Journal of Clinical Virology. 2010, 48(3), pp. 187-192. ISSN 1386-6532. eISSN 1873-5967. Available under: doi: 10.1016/j.jcv.2010.04.013

Zusammenfassung

Background
Real-time assays for Yellow fever virus (YFV) would help to improve acute diagnostics in outbreak investigations.

Objectives
To develop a real-time assay for YFV able to detect African and South American strains.

Study design
Three short probe (14–18 nt) formats were compared and a plasmid-transcribed RNA standard was used to test the performance of the assays. Additionally the new TaqM1 enzyme was tested.

Results
A locked nucleotide probe (LNA probe) performed best with an analytical sensitivity of 10 RNA molecules detected. 44 African and 10 South American strains were detectable. One South American strain from 1984 had a one-nucleotide deviation in the hybridisation sequence for which the LNA probe had to be adapted. Comparison of enzymes revealed that not all enzymes are suitable for LNA probes.

Conclusion
The developed LNA probe based YFV real-time PCR performed best in an enzyme mix and less efficient using multifunctional enzymes.

Zusammenfassung in einer weiteren Sprache

Fachgebiet (DDC)
540 Chemie

Schlagwörter

YFV, Real-time PCR, LNA probe, MGB probe, Eclipse probe, TaqM1 enzyme

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ISO 690WEIDMANN, Manfred, Ousmane FAYE, Oumar FAYE, Ramon KRANASTER, Andreas MARX, Marcio R. T. NUNES, Pedro F. C. VASCONCELOS, Frank T. HUFERT, Amadou A. SALL, 2010. Improved LNA probe-based assay for the detection of African and South American yellow fever virus strains. In: Journal of Clinical Virology. 2010, 48(3), pp. 187-192. ISSN 1386-6532. eISSN 1873-5967. Available under: doi: 10.1016/j.jcv.2010.04.013
BibTex
@article{Weidmann2010Impro-12601,
  year={2010},
  doi={10.1016/j.jcv.2010.04.013},
  title={Improved LNA probe-based assay for the detection of African and South American yellow fever virus strains},
  number={3},
  volume={48},
  issn={1386-6532},
  journal={Journal of Clinical Virology},
  pages={187--192},
  author={Weidmann, Manfred and Faye, Ousmane and Faye, Oumar and Kranaster, Ramon and Marx, Andreas and Nunes, Marcio R. T. and Vasconcelos, Pedro F. C. and Hufert, Frank T. and Sall, Amadou A.}
}
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