Assignment of charge movements to electrogenic reaction steps of Na,K-ATPase by analysis of salt effects on the kinetics of charge movements

Kurzanzeige
dc.contributor.authorSokolov, Valerij S.deu
dc.contributor.authorAyuyan, Artem G.deu
dc.contributor.authorApell, Hans-Jürgen
dc.date.accessioned2011-03-24T17:35:34Zdeu
dc.date.available2011-03-24T17:35:34Zdeu
dc.date.issued2001deu
dc.description.abstractNa,K-ATPase-enriched membrane fragments adsorbed to lipid bilayers were used to study electrogenic Na+ movements induced by anzyme phosphorylation when ATP was photo-relased from inactive caged ATP, and simultaneously by externally applied alterating voltages which allowed the measurement of small ATP-induced membrane admittance changes. A detailed analysis of frequency dependence of the capacitance and conductance increments showed that the observed peocess consists of more than one electrogenic step. The frequency dependence could be described by the sum of two Lorentzian functions and y constant term. Preferentially, the slower process showed a dependence on the ion concentration of choline salts with different anions. This observation matches their effect on the partition between two phosphoenzyme states of the Na,K-ATPase, as established previously.eng
dc.description.versionpublished
dc.format.mimetypeapplication/pdfdeu
dc.identifier.citationFirst publ. in: European Biophysics Journal 30 (2001), pp. 515-527deu
dc.identifier.doi10.1007/s002490100179
dc.identifier.ppn274025450deu
dc.identifier.urihttp://kops.uni-konstanz.de/handle/123456789/7585
dc.language.isoengdeu
dc.legacy.dateIssued2007deu
dc.rightsAttribution-NonCommercial-NoDerivs 2.0 Generic
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/2.0/
dc.subjectNA,K-ATPasedeu
dc.subjectIon movementdeu
dc.subjectElectrogenicitydeu
dc.subjectCapacitancedeu
dc.subjectTransport kineticsdeu
dc.subject.ddc570deu
dc.titleAssignment of charge movements to electrogenic reaction steps of Na,K-ATPase by analysis of salt effects on the kinetics of charge movementseng
dc.typeJOURNAL_ARTICLEdeu
dspace.entity.typePublication
kops.citation.bibtex
@article{Sokolov2001Assig-7585,
  year={2001},
  doi={10.1007/s002490100179},
  title={Assignment of charge movements to electrogenic reaction steps of Na,K-ATPase by analysis of salt effects on the kinetics of charge movements},
  number={7},
  volume={30},
  issn={0175-7571},
  journal={European Biophysics Journal},
  pages={515--527},
  author={Sokolov, Valerij S. and Ayuyan, Artem G. and Apell, Hans-Jürgen}
}
kops.citation.iso690SOKOLOV, Valerij S., Artem G. AYUYAN, Hans-Jürgen APELL, 2001. Assignment of charge movements to electrogenic reaction steps of Na,K-ATPase by analysis of salt effects on the kinetics of charge movements. In: European Biophysics Journal. 2001, 30(7), pp. 515-527. ISSN 0175-7571. eISSN 1432-1017. Available under: doi: 10.1007/s002490100179deu
kops.citation.iso690SOKOLOV, Valerij S., Artem G. AYUYAN, Hans-Jürgen APELL, 2001. Assignment of charge movements to electrogenic reaction steps of Na,K-ATPase by analysis of salt effects on the kinetics of charge movements. In: European Biophysics Journal. 2001, 30(7), pp. 515-527. ISSN 0175-7571. eISSN 1432-1017. Available under: doi: 10.1007/s002490100179eng
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