Publikation:

Assignment of charge movements to electrogenic reaction steps of Na,K-ATPase by analysis of salt effects on the kinetics of charge movements

Lade...
Vorschaubild

Datum

2001

Autor:innen

Sokolov, Valerij S.
Ayuyan, Artem G.
Apell, Hans-Jürgen

Herausgeber:innen

Kontakt

ISSN der Zeitschrift

Electronic ISSN

ISBN

Bibliografische Daten

Verlag

Schriftenreihe

Auflagebezeichnung

ArXiv-ID

Internationale Patentnummer

Angaben zur Forschungsförderung

Projekt

Open Access-Veröffentlichung
Open Access Green
Core Facility der Universität Konstanz

Gesperrt bis

Titel in einer weiteren Sprache

Publikationstyp
Zeitschriftenartikel
Publikationsstatus
Published

Erschienen in

European Biophysics Journal. 2001, 30(7), pp. 515-527. ISSN 0175-7571. eISSN 1432-1017. Available under: doi: 10.1007/s002490100179

Zusammenfassung

Na,K-ATPase-enriched membrane fragments adsorbed to lipid bilayers were used to study electrogenic Na+ movements induced by anzyme phosphorylation when ATP was photo-relased from inactive caged ATP, and simultaneously by externally applied alterating voltages which allowed the measurement of small ATP-induced membrane admittance changes. A detailed analysis of frequency dependence of the capacitance and conductance increments showed that the observed peocess consists of more than one electrogenic step. The frequency dependence could be described by the sum of two Lorentzian functions and y constant term. Preferentially, the slower process showed a dependence on the ion concentration of choline salts with different anions. This observation matches their effect on the partition between two phosphoenzyme states of the Na,K-ATPase, as established previously.

Zusammenfassung in einer weiteren Sprache

Fachgebiet (DDC)
570 Biowissenschaften, Biologie

Schlagwörter

NA,K-ATPase, Ion movement, Electrogenicity, Capacitance, Transport kinetics

Konferenz

Rezension
undefined / . - undefined, undefined

Forschungsvorhaben

Organisationseinheiten

Zeitschriftenheft

Zugehörige Datensätze in KOPS

Zitieren

ISO 690SOKOLOV, Valerij S., Artem G. AYUYAN, Hans-Jürgen APELL, 2001. Assignment of charge movements to electrogenic reaction steps of Na,K-ATPase by analysis of salt effects on the kinetics of charge movements. In: European Biophysics Journal. 2001, 30(7), pp. 515-527. ISSN 0175-7571. eISSN 1432-1017. Available under: doi: 10.1007/s002490100179
BibTex
@article{Sokolov2001Assig-7585,
  year={2001},
  doi={10.1007/s002490100179},
  title={Assignment of charge movements to electrogenic reaction steps of Na,K-ATPase by analysis of salt effects on the kinetics of charge movements},
  number={7},
  volume={30},
  issn={0175-7571},
  journal={European Biophysics Journal},
  pages={515--527},
  author={Sokolov, Valerij S. and Ayuyan, Artem G. and Apell, Hans-Jürgen}
}
RDF
<rdf:RDF
    xmlns:dcterms="http://purl.org/dc/terms/"
    xmlns:dc="http://purl.org/dc/elements/1.1/"
    xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#"
    xmlns:bibo="http://purl.org/ontology/bibo/"
    xmlns:dspace="http://digital-repositories.org/ontologies/dspace/0.1.0#"
    xmlns:foaf="http://xmlns.com/foaf/0.1/"
    xmlns:void="http://rdfs.org/ns/void#"
    xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > 
  <rdf:Description rdf:about="https://kops.uni-konstanz.de/server/rdf/resource/123456789/7585">
    <dcterms:isPartOf rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/28"/>
    <dc:creator>Sokolov, Valerij S.</dc:creator>
    <foaf:homepage rdf:resource="http://localhost:8080/"/>
    <dc:contributor>Sokolov, Valerij S.</dc:contributor>
    <void:sparqlEndpoint rdf:resource="http://localhost/fuseki/dspace/sparql"/>
    <dspace:isPartOfCollection rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/28"/>
    <dc:contributor>Ayuyan, Artem G.</dc:contributor>
    <dcterms:hasPart rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/7585/1/Assignment_of_charge_movements_to_electrogenic_reaction_steps.pdf"/>
    <dc:language>eng</dc:language>
    <dc:creator>Apell, Hans-Jürgen</dc:creator>
    <dspace:hasBitstream rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/7585/1/Assignment_of_charge_movements_to_electrogenic_reaction_steps.pdf"/>
    <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-24T17:35:34Z</dc:date>
    <dcterms:abstract xml:lang="eng">Na,K-ATPase-enriched membrane fragments adsorbed to lipid bilayers were used to study electrogenic Na+ movements induced by anzyme phosphorylation when ATP was photo-relased from inactive caged ATP, and simultaneously by externally applied alterating voltages which allowed the measurement of small ATP-induced membrane admittance changes. A detailed analysis of frequency dependence of the capacitance and conductance increments showed that the observed peocess consists of more than one electrogenic step. The frequency dependence could be described by the sum of two Lorentzian functions and y constant term. Preferentially, the slower process showed a dependence on the ion concentration of choline salts with different anions. This observation matches their effect on the partition between two phosphoenzyme states of the Na,K-ATPase, as established previously.</dcterms:abstract>
    <dc:creator>Ayuyan, Artem G.</dc:creator>
    <dcterms:title>Assignment of charge movements to electrogenic reaction steps of Na,K-ATPase by analysis of salt effects on the kinetics of charge movements</dcterms:title>
    <dcterms:bibliographicCitation>First publ. in: European Biophysics Journal 30 (2001), pp. 515-527</dcterms:bibliographicCitation>
    <dc:contributor>Apell, Hans-Jürgen</dc:contributor>
    <bibo:uri rdf:resource="http://kops.uni-konstanz.de/handle/123456789/7585"/>
    <dc:rights>Attribution-NonCommercial-NoDerivs 2.0 Generic</dc:rights>
    <dc:format>application/pdf</dc:format>
    <dcterms:rights rdf:resource="http://creativecommons.org/licenses/by-nc-nd/2.0/"/>
    <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-24T17:35:34Z</dcterms:available>
    <dcterms:issued>2001</dcterms:issued>
  </rdf:Description>
</rdf:RDF>

Interner Vermerk

xmlui.Submission.submit.DescribeStep.inputForms.label.kops_note_fromSubmitter

Kontakt
URL der Originalveröffentl.

Prüfdatum der URL

Prüfungsdatum der Dissertation

Finanzierungsart

Kommentar zur Publikation

Allianzlizenz
Corresponding Authors der Uni Konstanz vorhanden
Internationale Co-Autor:innen
Universitätsbibliographie
Ja
Begutachtet
Diese Publikation teilen