Bioluminescence Emission from the Reaction of Luciferase-Flavin Mononucleotide Radical with Opt

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dc.contributor.authorKurfürst, Manfreddeu
dc.contributor.authorGhisla, Sandro
dc.contributor.authorHastings, J. Woodlanddeu
dc.date.accessioned2011-03-24T17:30:06Zdeu
dc.date.available2011-03-24T17:30:06Zdeu
dc.date.issued1983deu
dc.description.abstractThe blue neutral luciferase flavin radical has been shown not to be in a catalytically significant equilibrium with species leading to emission of light [Kurfurst, M., Ghisla, S., Presswood, R., & Hastings, J. W. (1982) Eur. J. Biochem. 123, 355-361]. It is shown here that this radical can nevertheless react with 02¯● to form a species that is competent in light emission. From its properties, the species formed is deduced to be luciferase-FMNH 4a-hydroperoxide, a key intermediate in the normal luciferase reaction. Although it is concluded that this intermediate can undergo a reversible homolytic dissociation to yield free superoxide and the corresponding luciferase radical, the slowness of these steps precludes a catalytic significance for these pathways in the normal bioluminescent reaction.eng
dc.description.versionpublished
dc.format.mimetypeapplication/pdfdeu
dc.identifier.citationFirst publ. in: Biochemistry 22 (1983), 7, pp. 1521-1525deu
dc.identifier.ppn281445753deu
dc.identifier.urihttp://kops.uni-konstanz.de/handle/123456789/6912
dc.language.isoengdeu
dc.legacy.dateIssued2008deu
dc.rightsAttribution-NonCommercial-NoDerivs 2.0 Generic
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/2.0/
dc.subject.ddc570deu
dc.titleBioluminescence Emission from the Reaction of Luciferase-Flavin Mononucleotide Radical with Opteng
dc.typeJOURNAL_ARTICLEdeu
dspace.entity.typePublication
kops.citation.bibtex
@article{Kurfurst1983Biolu-6912,
  year={1983},
  title={Bioluminescence Emission from the Reaction of Luciferase-Flavin Mononucleotide Radical with Opt},
  number={7},
  volume={22},
  issn={0006-2960},
  journal={Biochemistry},
  pages={1521--1525},
  author={Kurfürst, Manfred and Ghisla, Sandro and Hastings, J. Woodland}
}
kops.citation.iso690KURFÜRST, Manfred, Sandro GHISLA, J. Woodland HASTINGS, 1983. Bioluminescence Emission from the Reaction of Luciferase-Flavin Mononucleotide Radical with Opt. In: Biochemistry. 1983, 22(7), pp. 1521-1525. ISSN 0006-2960. eISSN 1520-4995deu
kops.citation.iso690KURFÜRST, Manfred, Sandro GHISLA, J. Woodland HASTINGS, 1983. Bioluminescence Emission from the Reaction of Luciferase-Flavin Mononucleotide Radical with Opt. In: Biochemistry. 1983, 22(7), pp. 1521-1525. ISSN 0006-2960. eISSN 1520-4995eng
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kops.sourcefieldBiochemistry. 1983, <b>22</b>(7), pp. 1521-1525. ISSN 0006-2960. eISSN 1520-4995deu
kops.sourcefield.plainBiochemistry. 1983, 22(7), pp. 1521-1525. ISSN 0006-2960. eISSN 1520-4995deu
kops.sourcefield.plainBiochemistry. 1983, 22(7), pp. 1521-1525. ISSN 0006-2960. eISSN 1520-4995eng
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source.periodicalTitleBiochemistry

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