Publikation:

Bioluminescence Emission from the Reaction of Luciferase-Flavin Mononucleotide Radical with Opt

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1983

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Kurfürst, Manfred
Hastings, J. Woodland

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Biochemistry. 1983, 22(7), pp. 1521-1525. ISSN 0006-2960. eISSN 1520-4995

Zusammenfassung

The blue neutral luciferase flavin radical has been shown not to be in a catalytically significant equilibrium with species leading to emission of light [Kurfurst, M., Ghisla, S., Presswood, R., & Hastings, J. W. (1982) Eur. J. Biochem. 123, 355-361]. It is shown here that this radical can nevertheless react with 02¯● to form a species that is competent in light emission. From its properties, the species formed is deduced to be luciferase-FMNH 4a-hydroperoxide, a key intermediate in the normal luciferase reaction. Although it is concluded that this intermediate can undergo a reversible homolytic dissociation to yield free superoxide and the corresponding luciferase radical, the slowness of these steps precludes a catalytic significance for these pathways in the normal bioluminescent reaction.

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570 Biowissenschaften, Biologie

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ISO 690KURFÜRST, Manfred, Sandro GHISLA, J. Woodland HASTINGS, 1983. Bioluminescence Emission from the Reaction of Luciferase-Flavin Mononucleotide Radical with Opt. In: Biochemistry. 1983, 22(7), pp. 1521-1525. ISSN 0006-2960. eISSN 1520-4995
BibTex
@article{Kurfurst1983Biolu-6912,
  year={1983},
  title={Bioluminescence Emission from the Reaction of Luciferase-Flavin Mononucleotide Radical with Opt},
  number={7},
  volume={22},
  issn={0006-2960},
  journal={Biochemistry},
  pages={1521--1525},
  author={Kurfürst, Manfred and Ghisla, Sandro and Hastings, J. Woodland}
}
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