Publikation: Bioluminescence Emission from the Reaction of Luciferase-Flavin Mononucleotide Radical with Opt
Dateien
Datum
Autor:innen
Herausgeber:innen
ISSN der Zeitschrift
Electronic ISSN
ISBN
Bibliografische Daten
Verlag
Schriftenreihe
Auflagebezeichnung
URI (zitierfähiger Link)
Internationale Patentnummer
Link zur Lizenz
Angaben zur Forschungsförderung
Projekt
Open Access-Veröffentlichung
Sammlungen
Core Facility der Universität Konstanz
Titel in einer weiteren Sprache
Publikationstyp
Publikationsstatus
Erschienen in
Zusammenfassung
The blue neutral luciferase flavin radical has been shown not to be in a catalytically significant equilibrium with species leading to emission of light [Kurfurst, M., Ghisla, S., Presswood, R., & Hastings, J. W. (1982) Eur. J. Biochem. 123, 355-361]. It is shown here that this radical can nevertheless react with 02¯● to form a species that is competent in light emission. From its properties, the species formed is deduced to be luciferase-FMNH 4a-hydroperoxide, a key intermediate in the normal luciferase reaction. Although it is concluded that this intermediate can undergo a reversible homolytic dissociation to yield free superoxide and the corresponding luciferase radical, the slowness of these steps precludes a catalytic significance for these pathways in the normal bioluminescent reaction.
Zusammenfassung in einer weiteren Sprache
Fachgebiet (DDC)
Schlagwörter
Konferenz
Rezension
Zitieren
ISO 690
KURFÜRST, Manfred, Sandro GHISLA, J. Woodland HASTINGS, 1983. Bioluminescence Emission from the Reaction of Luciferase-Flavin Mononucleotide Radical with Opt. In: Biochemistry. 1983, 22(7), pp. 1521-1525. ISSN 0006-2960. eISSN 1520-4995BibTex
@article{Kurfurst1983Biolu-6912, year={1983}, title={Bioluminescence Emission from the Reaction of Luciferase-Flavin Mononucleotide Radical with Opt}, number={7}, volume={22}, issn={0006-2960}, journal={Biochemistry}, pages={1521--1525}, author={Kurfürst, Manfred and Ghisla, Sandro and Hastings, J. Woodland} }
RDF
<rdf:RDF xmlns:dcterms="http://purl.org/dc/terms/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#" xmlns:bibo="http://purl.org/ontology/bibo/" xmlns:dspace="http://digital-repositories.org/ontologies/dspace/0.1.0#" xmlns:foaf="http://xmlns.com/foaf/0.1/" xmlns:void="http://rdfs.org/ns/void#" xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > <rdf:Description rdf:about="https://kops.uni-konstanz.de/server/rdf/resource/123456789/6912"> <dspace:hasBitstream rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/6912/1/Biochemistry_1983_KurfuerstBioluminescence_emission_from_the_reac.pdf"/> <dcterms:rights rdf:resource="http://creativecommons.org/licenses/by-nc-nd/2.0/"/> <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-24T17:30:06Z</dc:date> <dcterms:abstract xml:lang="eng">The blue neutral luciferase flavin radical has been shown not to be in a catalytically significant equilibrium with species leading to emission of light [Kurfurst, M., Ghisla, S., Presswood, R., & Hastings, J. W. (1982) Eur. J. Biochem. 123, 355-361]. It is shown here that this radical can nevertheless react with 02¯● to form a species that is competent in light emission. From its properties, the species formed is deduced to be luciferase-FMNH 4a-hydroperoxide, a key intermediate in the normal luciferase reaction. Although it is concluded that this intermediate can undergo a reversible homolytic dissociation to yield free superoxide and the corresponding luciferase radical, the slowness of these steps precludes a catalytic significance for these pathways in the normal bioluminescent reaction.</dcterms:abstract> <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-24T17:30:06Z</dcterms:available> <dc:rights>Attribution-NonCommercial-NoDerivs 2.0 Generic</dc:rights> <dcterms:hasPart rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/6912/1/Biochemistry_1983_KurfuerstBioluminescence_emission_from_the_reac.pdf"/> <dspace:isPartOfCollection rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/28"/> <dc:contributor>Hastings, J. Woodland</dc:contributor> <dc:creator>Hastings, J. Woodland</dc:creator> <foaf:homepage rdf:resource="http://localhost:8080/"/> <dcterms:issued>1983</dcterms:issued> <void:sparqlEndpoint rdf:resource="http://localhost/fuseki/dspace/sparql"/> <dc:contributor>Kurfürst, Manfred</dc:contributor> <dc:format>application/pdf</dc:format> <bibo:uri rdf:resource="http://kops.uni-konstanz.de/handle/123456789/6912"/> <dc:creator>Ghisla, Sandro</dc:creator> <dcterms:isPartOf rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/28"/> <dcterms:title>Bioluminescence Emission from the Reaction of Luciferase-Flavin Mononucleotide Radical with Opt</dcterms:title> <dcterms:bibliographicCitation>First publ. in: Biochemistry 22 (1983), 7, pp. 1521-1525</dcterms:bibliographicCitation> <dc:creator>Kurfürst, Manfred</dc:creator> <dc:contributor>Ghisla, Sandro</dc:contributor> <dc:language>eng</dc:language> </rdf:Description> </rdf:RDF>