Lessons from GNE-deficient embryonic stem cells : sialic acid biosynthesis is involved in proliferation and gene expression

Kurzanzeige
dc.contributor.authorWeidemann, Wenke
dc.contributor.authorKlukas, Christian
dc.contributor.authorKlein, Andreas
dc.contributor.authorSimm, Andreas
dc.contributor.authorSchreiber, Falk
dc.contributor.authorHorstkorte, Rüdiger
dc.date.accessioned2017-10-25T09:07:07Z
dc.date.available2017-10-25T09:07:07Z
dc.date.issued2010eng
dc.description.abstractSialic acids are widely expressed as terminal carbohydrates on glycoconjugates of eukaryotic cells. They are involved in a variety of cellular functions, such as cell adhesion or signal recognition. The key enzyme of sialic acid biosynthesis is the bifunctional UDP-N-acetylglucosamine-2-epimerase/N-acetylmannosamine kinase (GNE), which catalyzes the first two steps of sialic acid biosynthesis in the cytosol. Inactivation of GNE causes early embryonic lethality. In this study, we analyzed wild-type and GNE-deficient embryonic stem cells from mice. We found for the first time that proliferation is directly correlated with GNE-expression and the cellular sialic acid concentration. Furthermore, we identified growth-related genes that are differentially expressed in GNE-deficient embryonic stem cells compared to wild-type embryonic stem cells.eng
dc.description.versionpublishedeng
dc.identifier.doi10.1093/glycob/cwp153eng
dc.identifier.pmid19797319eng
dc.identifier.urihttps://kops.uni-konstanz.de/handle/123456789/40405
dc.language.isoengeng
dc.subjectcell differentiation, N -acetylmannosamine , N -acetylmannosamine kinase , UDP- N -acetylglucosamine-2- epimeraseeng
dc.subject.ddc004eng
dc.titleLessons from GNE-deficient embryonic stem cells : sialic acid biosynthesis is involved in proliferation and gene expressioneng
dc.typeJOURNAL_ARTICLEeng
dspace.entity.typePublication
kops.citation.bibtex
@article{Weidemann2010Lesso-40405,
  year={2010},
  doi={10.1093/glycob/cwp153},
  title={Lessons from GNE-deficient embryonic stem cells : sialic acid biosynthesis is involved in proliferation and gene expression},
  number={1},
  volume={20},
  issn={0959-6658},
  journal={Glycobiology},
  pages={107--117},
  author={Weidemann, Wenke and Klukas, Christian and Klein, Andreas and Simm, Andreas and Schreiber, Falk and Horstkorte, Rüdiger}
}
kops.citation.iso690WEIDEMANN, Wenke, Christian KLUKAS, Andreas KLEIN, Andreas SIMM, Falk SCHREIBER, Rüdiger HORSTKORTE, 2010. Lessons from GNE-deficient embryonic stem cells : sialic acid biosynthesis is involved in proliferation and gene expression. In: Glycobiology. 2010, 20(1), pp. 107-117. ISSN 0959-6658. eISSN 1460-2423. Available under: doi: 10.1093/glycob/cwp153deu
kops.citation.iso690WEIDEMANN, Wenke, Christian KLUKAS, Andreas KLEIN, Andreas SIMM, Falk SCHREIBER, Rüdiger HORSTKORTE, 2010. Lessons from GNE-deficient embryonic stem cells : sialic acid biosynthesis is involved in proliferation and gene expression. In: Glycobiology. 2010, 20(1), pp. 107-117. ISSN 0959-6658. eISSN 1460-2423. Available under: doi: 10.1093/glycob/cwp153eng
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