Identification of the Luciferase-Bound Flavin-4A-Hydroxide as the Primary Emitter in the Bacterial Bioluminescence Reaction

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dc.contributor.authorKurfürst, Manfreddeu
dc.contributor.authorHastings, J. Woodlanddeu
dc.contributor.authorGhisla, Sandro
dc.contributor.authorMacheroux, Peterdeu
dc.date.accessioned2011-03-24T17:28:27Zdeu
dc.date.available2011-03-24T17:28:27Zdeu
dc.date.issued1984deu
dc.description.abstractThe luciferase light-emitting reaction was carried out at 1°C by mixing purified luciferase-FMN-4a-hydroperoxide with long chain aldehyde (decanal). Simultaneous kinetic measurements of bioluminescence and absorbance showed that light emission decayed more rapidly than oxidized FMN appeared, indicative of a transient intermediate species subsequent to light emission. The same species was found in reaction mixtures examined immediately after light emission was completed. Both its absorption spectrum (λmax, 360nm) and its fluorescence emission (λmax, 490nm) are consistent with the hypothesis that the chromophore is the luciferase-bound flavin-4a-hydroxide, the ground state of the primary emitter in the reaction. It has a relatively short lifetime (7 min at 9°C) and decays to the stable product, FMN, by losing water. The activation energy for this step was determined to be 83 kJ mol-1.eng
dc.description.versionpublished
dc.format.mimetypeapplication/pdfdeu
dc.identifier.citationFirst publ. in: Flavins and flavoproteins / S. G. Mayhew (ed.) - Berlin: de Gruyter, 1984, pp. 657-667deu
dc.identifier.ppn287850188deu
dc.identifier.urihttp://kops.uni-konstanz.de/handle/123456789/6696
dc.language.isoengdeu
dc.legacy.dateIssued2008deu
dc.rightsAttribution-NonCommercial-NoDerivs 2.0 Generic
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/2.0/
dc.subject.ddc570deu
dc.titleIdentification of the Luciferase-Bound Flavin-4A-Hydroxide as the Primary Emitter in the Bacterial Bioluminescence Reactioneng
dc.typeINCOLLECTIONdeu
dspace.entity.typePublication
kops.citation.bibtex
@incollection{Kurfurst1984Ident-6696,
  year={1984},
  title={Identification of the Luciferase-Bound Flavin-4A-Hydroxide as the Primary Emitter in the Bacterial Bioluminescence Reaction},
  publisher={de Gruyter},
  address={Berlin},
  booktitle={Flavins and flavoproteins},
  pages={657--667},
  editor={Mayhew, S. G.},
  author={Kurfürst, Manfred and Hastings, J. Woodland and Ghisla, Sandro and Macheroux, Peter}
}
kops.citation.iso690KURFÜRST, Manfred, J. Woodland HASTINGS, Sandro GHISLA, Peter MACHEROUX, 1984. Identification of the Luciferase-Bound Flavin-4A-Hydroxide as the Primary Emitter in the Bacterial Bioluminescence Reaction. In: MAYHEW, S. G., ed.. Flavins and flavoproteins. Berlin: de Gruyter, 1984, pp. 657-667deu
kops.citation.iso690KURFÜRST, Manfred, J. Woodland HASTINGS, Sandro GHISLA, Peter MACHEROUX, 1984. Identification of the Luciferase-Bound Flavin-4A-Hydroxide as the Primary Emitter in the Bacterial Bioluminescence Reaction. In: MAYHEW, S. G., ed.. Flavins and flavoproteins. Berlin: de Gruyter, 1984, pp. 657-667eng
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kops.sourcefieldMAYHEW, S. G., ed.. <i>Flavins and flavoproteins</i>. Berlin: de Gruyter, 1984, pp. 657-667deu
kops.sourcefield.plainMAYHEW, S. G., ed.. Flavins and flavoproteins. Berlin: de Gruyter, 1984, pp. 657-667deu
kops.sourcefield.plainMAYHEW, S. G., ed.. Flavins and flavoproteins. Berlin: de Gruyter, 1984, pp. 657-667eng
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source.contributor.editorMayhew, S. G.
source.publisherde Gruyter
source.publisher.locationBerlin
source.titleFlavins and flavoproteins

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