Publikation: Identification of the Luciferase-Bound Flavin-4A-Hydroxide as the Primary Emitter in the Bacterial Bioluminescence Reaction
Dateien
Datum
Autor:innen
Herausgeber:innen
ISSN der Zeitschrift
Electronic ISSN
ISBN
Bibliografische Daten
Verlag
Schriftenreihe
Auflagebezeichnung
URI (zitierfähiger Link)
Internationale Patentnummer
Link zur Lizenz
Angaben zur Forschungsförderung
Projekt
Open Access-Veröffentlichung
Sammlungen
Core Facility der Universität Konstanz
Titel in einer weiteren Sprache
Publikationstyp
Publikationsstatus
Erschienen in
Zusammenfassung
The luciferase light-emitting reaction was carried out at 1°C by mixing purified luciferase-FMN-4a-hydroperoxide with long chain aldehyde (decanal). Simultaneous kinetic measurements of bioluminescence and absorbance showed that light emission decayed more rapidly than oxidized FMN appeared, indicative of a transient intermediate species subsequent to light emission. The same species was found in reaction mixtures examined immediately after light emission was completed. Both its absorption spectrum (λmax, 360nm) and its fluorescence emission (λmax, 490nm) are consistent with the hypothesis that the chromophore is the luciferase-bound flavin-4a-hydroxide, the ground state of the primary emitter in the reaction. It has a relatively short lifetime (7 min at 9°C) and decays to the stable product, FMN, by losing water. The activation energy for this step was determined to be 83 kJ mol-1.
Zusammenfassung in einer weiteren Sprache
Fachgebiet (DDC)
Schlagwörter
Konferenz
Rezension
Zitieren
ISO 690
KURFÜRST, Manfred, J. Woodland HASTINGS, Sandro GHISLA, Peter MACHEROUX, 1984. Identification of the Luciferase-Bound Flavin-4A-Hydroxide as the Primary Emitter in the Bacterial Bioluminescence Reaction. In: MAYHEW, S. G., ed.. Flavins and flavoproteins. Berlin: de Gruyter, 1984, pp. 657-667BibTex
@incollection{Kurfurst1984Ident-6696, year={1984}, title={Identification of the Luciferase-Bound Flavin-4A-Hydroxide as the Primary Emitter in the Bacterial Bioluminescence Reaction}, publisher={de Gruyter}, address={Berlin}, booktitle={Flavins and flavoproteins}, pages={657--667}, editor={Mayhew, S. G.}, author={Kurfürst, Manfred and Hastings, J. Woodland and Ghisla, Sandro and Macheroux, Peter} }
RDF
<rdf:RDF xmlns:dcterms="http://purl.org/dc/terms/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#" xmlns:bibo="http://purl.org/ontology/bibo/" xmlns:dspace="http://digital-repositories.org/ontologies/dspace/0.1.0#" xmlns:foaf="http://xmlns.com/foaf/0.1/" xmlns:void="http://rdfs.org/ns/void#" xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > <rdf:Description rdf:about="https://kops.uni-konstanz.de/server/rdf/resource/123456789/6696"> <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-24T17:28:27Z</dc:date> <dc:creator>Kurfürst, Manfred</dc:creator> <bibo:uri rdf:resource="http://kops.uni-konstanz.de/handle/123456789/6696"/> <dc:creator>Ghisla, Sandro</dc:creator> <dcterms:bibliographicCitation>First publ. in: Flavins and flavoproteins / S. G. Mayhew (ed.) - Berlin: de Gruyter, 1984, pp. 657-667</dcterms:bibliographicCitation> <dc:contributor>Macheroux, Peter</dc:contributor> <dc:format>application/pdf</dc:format> <dcterms:isPartOf rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/28"/> <foaf:homepage rdf:resource="http://localhost:8080/"/> <dcterms:title>Identification of the Luciferase-Bound Flavin-4A-Hydroxide as the Primary Emitter in the Bacterial Bioluminescence Reaction</dcterms:title> <void:sparqlEndpoint rdf:resource="http://localhost/fuseki/dspace/sparql"/> <dc:language>eng</dc:language> <dcterms:issued>1984</dcterms:issued> <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-24T17:28:27Z</dcterms:available> <dc:rights>Attribution-NonCommercial-NoDerivs 2.0 Generic</dc:rights> <dspace:hasBitstream rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/6696/1/84Ghisla.pdf"/> <dcterms:abstract xml:lang="eng">The luciferase light-emitting reaction was carried out at 1°C by mixing purified luciferase-FMN-4a-hydroperoxide with long chain aldehyde (decanal). Simultaneous kinetic measurements of bioluminescence and absorbance showed that light emission decayed more rapidly than oxidized FMN appeared, indicative of a transient intermediate species subsequent to light emission. The same species was found in reaction mixtures examined immediately after light emission was completed. Both its absorption spectrum (λmax, 360nm) and its fluorescence emission (λmax, 490nm) are consistent with the hypothesis that the chromophore is the luciferase-bound flavin-4a-hydroxide, the ground state of the primary emitter in the reaction. It has a relatively short lifetime (7 min at 9°C) and decays to the stable product, FMN, by losing water. The activation energy for this step was determined to be 83 kJ mol-1.</dcterms:abstract> <dc:creator>Macheroux, Peter</dc:creator> <dc:contributor>Hastings, J. Woodland</dc:contributor> <dc:creator>Hastings, J. Woodland</dc:creator> <dspace:isPartOfCollection rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/28"/> <dcterms:rights rdf:resource="http://creativecommons.org/licenses/by-nc-nd/2.0/"/> <dc:contributor>Kurfürst, Manfred</dc:contributor> <dcterms:hasPart rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/6696/1/84Ghisla.pdf"/> <dc:contributor>Ghisla, Sandro</dc:contributor> </rdf:Description> </rdf:RDF>