Antigen-Bound and Free β-Amyloid Autoantibodies in Serum of Healthy Adults

Kurzanzeige
dc.contributor.authorMaftei, Madalina
dc.contributor.authorThurm, Franka
dc.contributor.authorLeirer, Vera Mariadeu
dc.contributor.authorArnim, Christine A. F. vondeu
dc.contributor.authorElbert, Thomas
dc.contributor.authorPrzybylski, Michael
dc.contributor.authorKolassa, Iris-Tatjana
dc.contributor.authorManea, Marilena
dc.date.accessioned2012-10-08T11:12:03Zdeu
dc.date.available2012-10-08T11:12:03Zdeu
dc.date.issued2012
dc.description.abstractPhysiological β-amyloid autoantibodies (Aβ-autoantibodies) are currently investigated as potential diagnostic and therapeutic tools for Alzheimer’s disease (AD). In previous studies, their determination in serum and cerebrospinal fluid (CSF) using indirect ELISA has provided controversial results, which may be due to the presence of preformed Aβ antigen-antibody immune complexes. Based on the epitope specificity of the Aβ-autoantibodies, recently elucidated in our laboratory, we developed (a) a sandwich ELISA for the determination of circulating Aβ-IgG immune complexes and (b) an indirect ELISA for the determination of free Aβ-autoantibodies. This methodology was applied to the analysis of serum samples from healthy individuals within the age range of 18 to 89 years. Neuropsychological examination of the participants in this study indicated non-pathological, age-related cognitive decline, revealed especially by tests of visual memory and executive function, as well as speed-related tasks. The ELISA serum determinations showed significantly higher levels of Aβ-IgG immune complexes compared to free Aβ-autoantibodies, while no correlation with age or cognitive performance of the participants was found.eng
dc.description.versionpublished
dc.identifier.citationPLoS ONE ; 7 (2012), 9. - e44516deu
dc.identifier.doi10.1371/journal.pone.0044516deu
dc.identifier.pmid22973459
dc.identifier.ppn371952174deu
dc.identifier.urihttp://kops.uni-konstanz.de/handle/123456789/20613
dc.language.isoengdeu
dc.legacy.dateIssued2012-10-08deu
dc.rightsterms-of-usedeu
dc.rights.urihttps://rightsstatements.org/page/InC/1.0/deu
dc.subject.ddc540deu
dc.titleAntigen-Bound and Free β-Amyloid Autoantibodies in Serum of Healthy Adultseng
dc.typeJOURNAL_ARTICLEdeu
dspace.entity.typePublication
kops.citation.bibtex
@article{Maftei2012Antig-20613,
  year={2012},
  doi={10.1371/journal.pone.0044516},
  title={Antigen-Bound and Free β-Amyloid Autoantibodies in Serum of Healthy Adults},
  number={9},
  volume={7},
  journal={PLoS ONE},
  author={Maftei, Madalina and Thurm, Franka and Leirer, Vera Maria and Arnim, Christine A. F. von and Elbert, Thomas and Przybylski, Michael and Kolassa, Iris-Tatjana and Manea, Marilena},
  note={Article Number: e44516}
}
kops.citation.iso690MAFTEI, Madalina, Franka THURM, Vera Maria LEIRER, Christine A. F. von ARNIM, Thomas ELBERT, Michael PRZYBYLSKI, Iris-Tatjana KOLASSA, Marilena MANEA, 2012. Antigen-Bound and Free β-Amyloid Autoantibodies in Serum of Healthy Adults. In: PLoS ONE. 2012, 7(9), e44516. eISSN 1932-6203. Available under: doi: 10.1371/journal.pone.0044516deu
kops.citation.iso690MAFTEI, Madalina, Franka THURM, Vera Maria LEIRER, Christine A. F. von ARNIM, Thomas ELBERT, Michael PRZYBYLSKI, Iris-Tatjana KOLASSA, Marilena MANEA, 2012. Antigen-Bound and Free β-Amyloid Autoantibodies in Serum of Healthy Adults. In: PLoS ONE. 2012, 7(9), e44516. eISSN 1932-6203. Available under: doi: 10.1371/journal.pone.0044516eng
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    <dcterms:abstract xml:lang="eng">Physiological β-amyloid autoantibodies (Aβ-autoantibodies) are currently investigated as potential diagnostic and therapeutic tools for Alzheimer’s disease (AD). In previous studies, their determination in serum and cerebrospinal fluid (CSF) using indirect ELISA has provided controversial results, which may be due to the presence of preformed Aβ antigen-antibody immune complexes. Based on the epitope specificity of the Aβ-autoantibodies, recently elucidated in our laboratory, we developed (a) a sandwich ELISA for the determination of circulating Aβ-IgG immune complexes and (b) an indirect ELISA for the determination of free Aβ-autoantibodies. This methodology was applied to the analysis of serum samples from healthy individuals within the age range of 18 to 89 years. Neuropsychological examination of the participants in this study indicated non-pathological, age-related cognitive decline, revealed especially by tests of visual memory and executive function, as well as speed-related tasks. The ELISA serum determinations showed significantly higher levels of Aβ-IgG immune complexes compared to free Aβ-autoantibodies, while no correlation with age or cognitive performance of the participants was found.</dcterms:abstract>
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kops.sourcefield.plainPLoS ONE. 2012, 7(9), e44516. eISSN 1932-6203. Available under: doi: 10.1371/journal.pone.0044516eng
kops.submitter.emailingrid.muench@uni-konstanz.dedeu
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