Antigen-Bound and Free β-Amyloid Autoantibodies in Serum of Healthy Adults

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PLoS ONE. 2012, 7(9), e44516. eISSN 1932-6203. Available under: doi: 10.1371/journal.pone.0044516
Zusammenfassung

Physiological β-amyloid autoantibodies (Aβ-autoantibodies) are currently investigated as potential diagnostic and therapeutic tools for Alzheimer’s disease (AD). In previous studies, their determination in serum and cerebrospinal fluid (CSF) using indirect ELISA has provided controversial results, which may be due to the presence of preformed Aβ antigen-antibody immune complexes. Based on the epitope specificity of the Aβ-autoantibodies, recently elucidated in our laboratory, we developed (a) a sandwich ELISA for the determination of circulating Aβ-IgG immune complexes and (b) an indirect ELISA for the determination of free Aβ-autoantibodies. This methodology was applied to the analysis of serum samples from healthy individuals within the age range of 18 to 89 years. Neuropsychological examination of the participants in this study indicated non-pathological, age-related cognitive decline, revealed especially by tests of visual memory and executive function, as well as speed-related tasks. The ELISA serum determinations showed significantly higher levels of Aβ-IgG immune complexes compared to free Aβ-autoantibodies, while no correlation with age or cognitive performance of the participants was found.

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ISO 690MAFTEI, Madalina, Franka THURM, Vera Maria LEIRER, Christine A. F. von ARNIM, Thomas ELBERT, Michael PRZYBYLSKI, Iris-Tatjana KOLASSA, Marilena MANEA, 2012. Antigen-Bound and Free β-Amyloid Autoantibodies in Serum of Healthy Adults. In: PLoS ONE. 2012, 7(9), e44516. eISSN 1932-6203. Available under: doi: 10.1371/journal.pone.0044516
BibTex
@article{Maftei2012Antig-20613,
  year={2012},
  doi={10.1371/journal.pone.0044516},
  title={Antigen-Bound and Free β-Amyloid Autoantibodies in Serum of Healthy Adults},
  number={9},
  volume={7},
  journal={PLoS ONE},
  author={Maftei, Madalina and Thurm, Franka and Leirer, Vera Maria and Arnim, Christine A. F. von and Elbert, Thomas and Przybylski, Michael and Kolassa, Iris-Tatjana and Manea, Marilena},
  note={Article Number: e44516}
}
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