Antigen-Bound and Free β-Amyloid Autoantibodies in Serum of Healthy Adults

Lade...
Vorschaubild
Dateien
maftei_etal_Antigen.pdf
maftei_etal_Antigen.pdfGröße: 777.12 KBDownloads: 369
Datum
2012
Herausgeber:innen
Kontakt
ISSN der Zeitschrift
Electronic ISSN
ISBN
Bibliografische Daten
Verlag
Schriftenreihe
Auflagebezeichnung
ArXiv-ID
Internationale Patentnummer
EU-Projektnummer
DFG-Projektnummer
Projekt
Open Access-Veröffentlichung
Gesperrt bis
Titel in einer weiteren Sprache
Forschungsvorhaben
Organisationseinheiten
Zeitschriftenheft
Publikationstyp
Zeitschriftenartikel
Publikationsstatus
unikn.publication.listelement.citation.prefix.version.undefined
PLoS ONE. 2012, 7(9), e44516. eISSN 1932-6203. Available under: doi: 10.1371/journal.pone.0044516
Zusammenfassung

Physiological β-amyloid autoantibodies (Aβ-autoantibodies) are currently investigated as potential diagnostic and therapeutic tools for Alzheimer’s disease (AD). In previous studies, their determination in serum and cerebrospinal fluid (CSF) using indirect ELISA has provided controversial results, which may be due to the presence of preformed Aβ antigen-antibody immune complexes. Based on the epitope specificity of the Aβ-autoantibodies, recently elucidated in our laboratory, we developed (a) a sandwich ELISA for the determination of circulating Aβ-IgG immune complexes and (b) an indirect ELISA for the determination of free Aβ-autoantibodies. This methodology was applied to the analysis of serum samples from healthy individuals within the age range of 18 to 89 years. Neuropsychological examination of the participants in this study indicated non-pathological, age-related cognitive decline, revealed especially by tests of visual memory and executive function, as well as speed-related tasks. The ELISA serum determinations showed significantly higher levels of Aβ-IgG immune complexes compared to free Aβ-autoantibodies, while no correlation with age or cognitive performance of the participants was found.

Zusammenfassung in einer weiteren Sprache
Fachgebiet (DDC)
540 Chemie
Schlagwörter
Konferenz
Rezension
undefined / . - undefined, undefined
Zitieren
ISO 690MAFTEI, Madalina, Franka THURM, Vera Maria LEIRER, Christine A. F. von ARNIM, Thomas ELBERT, Michael PRZYBYLSKI, Iris-Tatjana KOLASSA, Marilena MANEA, 2012. Antigen-Bound and Free β-Amyloid Autoantibodies in Serum of Healthy Adults. In: PLoS ONE. 2012, 7(9), e44516. eISSN 1932-6203. Available under: doi: 10.1371/journal.pone.0044516
BibTex
@article{Maftei2012Antig-20613,
  year={2012},
  doi={10.1371/journal.pone.0044516},
  title={Antigen-Bound and Free β-Amyloid Autoantibodies in Serum of Healthy Adults},
  number={9},
  volume={7},
  journal={PLoS ONE},
  author={Maftei, Madalina and Thurm, Franka and Leirer, Vera Maria and Arnim, Christine A. F. von and Elbert, Thomas and Przybylski, Michael and Kolassa, Iris-Tatjana and Manea, Marilena},
  note={Article Number: e44516}
}
RDF
<rdf:RDF
    xmlns:dcterms="http://purl.org/dc/terms/"
    xmlns:dc="http://purl.org/dc/elements/1.1/"
    xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#"
    xmlns:bibo="http://purl.org/ontology/bibo/"
    xmlns:dspace="http://digital-repositories.org/ontologies/dspace/0.1.0#"
    xmlns:foaf="http://xmlns.com/foaf/0.1/"
    xmlns:void="http://rdfs.org/ns/void#"
    xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > 
  <rdf:Description rdf:about="https://kops.uni-konstanz.de/server/rdf/resource/123456789/20613">
    <dc:contributor>Manea, Marilena</dc:contributor>
    <dc:creator>Maftei, Madalina</dc:creator>
    <dc:contributor>Thurm, Franka</dc:contributor>
    <dcterms:isPartOf rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/52"/>
    <dcterms:rights rdf:resource="https://rightsstatements.org/page/InC/1.0/"/>
    <dspace:isPartOfCollection rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/52"/>
    <dcterms:issued>2012</dcterms:issued>
    <dc:contributor>Arnim, Christine A. F. von</dc:contributor>
    <dc:creator>Manea, Marilena</dc:creator>
    <dspace:hasBitstream rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/20613/2/maftei_etal_Antigen.pdf"/>
    <dcterms:hasPart rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/20613/2/maftei_etal_Antigen.pdf"/>
    <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2012-10-08T11:12:03Z</dcterms:available>
    <dc:creator>Thurm, Franka</dc:creator>
    <dcterms:title>Antigen-Bound and Free β-Amyloid Autoantibodies in Serum of Healthy Adults</dcterms:title>
    <dcterms:isPartOf rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/29"/>
    <dspace:isPartOfCollection rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/29"/>
    <dc:contributor>Leirer, Vera Maria</dc:contributor>
    <dc:rights>terms-of-use</dc:rights>
    <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2012-10-08T11:12:03Z</dc:date>
    <dcterms:bibliographicCitation>PLoS ONE ; 7 (2012), 9. - e44516</dcterms:bibliographicCitation>
    <void:sparqlEndpoint rdf:resource="http://localhost/fuseki/dspace/sparql"/>
    <dc:creator>Arnim, Christine A. F. von</dc:creator>
    <foaf:homepage rdf:resource="http://localhost:8080/"/>
    <dc:creator>Elbert, Thomas</dc:creator>
    <dc:contributor>Przybylski, Michael</dc:contributor>
    <dc:creator>Leirer, Vera Maria</dc:creator>
    <dcterms:abstract xml:lang="eng">Physiological β-amyloid autoantibodies (Aβ-autoantibodies) are currently investigated as potential diagnostic and therapeutic tools for Alzheimer’s disease (AD). In previous studies, their determination in serum and cerebrospinal fluid (CSF) using indirect ELISA has provided controversial results, which may be due to the presence of preformed Aβ antigen-antibody immune complexes. Based on the epitope specificity of the Aβ-autoantibodies, recently elucidated in our laboratory, we developed (a) a sandwich ELISA for the determination of circulating Aβ-IgG immune complexes and (b) an indirect ELISA for the determination of free Aβ-autoantibodies. This methodology was applied to the analysis of serum samples from healthy individuals within the age range of 18 to 89 years. Neuropsychological examination of the participants in this study indicated non-pathological, age-related cognitive decline, revealed especially by tests of visual memory and executive function, as well as speed-related tasks. The ELISA serum determinations showed significantly higher levels of Aβ-IgG immune complexes compared to free Aβ-autoantibodies, while no correlation with age or cognitive performance of the participants was found.</dcterms:abstract>
    <dc:contributor>Maftei, Madalina</dc:contributor>
    <dc:contributor>Kolassa, Iris-Tatjana</dc:contributor>
    <dc:contributor>Elbert, Thomas</dc:contributor>
    <dc:creator>Kolassa, Iris-Tatjana</dc:creator>
    <bibo:uri rdf:resource="http://kops.uni-konstanz.de/handle/123456789/20613"/>
    <dc:language>eng</dc:language>
    <dc:creator>Przybylski, Michael</dc:creator>
  </rdf:Description>
</rdf:RDF>
Interner Vermerk
xmlui.Submission.submit.DescribeStep.inputForms.label.kops_note_fromSubmitter
Kontakt
URL der Originalveröffentl.
Prüfdatum der URL
Prüfungsdatum der Dissertation
Finanzierungsart
Kommentar zur Publikation
Allianzlizenz
Corresponding Authors der Uni Konstanz vorhanden
Internationale Co-Autor:innen
Universitätsbibliographie
Ja
Begutachtet