Publikation:

Antigen-Bound and Free β-Amyloid Autoantibodies in Serum of Healthy Adults

Vorschaubild

Dateien

maftei_etal_Antigen.pdf
maftei_etal_Antigen.pdfGröße: 777.12 KBDownloads: 387

Datum

2012

Autor:innen

Leirer, Vera Maria
Arnim, Christine A. F. von

Herausgeber:innen

Kontakt

ISSN der Zeitschrift

Electronic ISSN

ISBN

Bibliografische Daten

Verlag

Schriftenreihe

Auflagebezeichnung

URI (zitierfähiger Link)
http://nbn-resolving.de/urn:nbn:de:bsz:352-206138
DOI (zitierfähiger Link)
https://doi.org/10.1371/journal.pone.0044516
ArXiv-ID

Internationale Patentnummer

Link zur Lizenz
Urheberrechtlich geschützt

Angaben zur Forschungsförderung

Projekt

Open Access-Veröffentlichung
Open Access Gold

Sammlungen

Chemie: Publikationen
Zukunftskolleg: Publikationen
Core Facility der Universität Konstanz

Gesperrt bis

Titel in einer weiteren Sprache

Publikationstyp
Zeitschriftenartikel
Publikationsstatus
Published

Erschienen in

PLoS ONE. 2012, 7(9), e44516. eISSN 1932-6203. Available under: doi: 10.1371/journal.pone.0044516

Zusammenfassung

Physiological β-amyloid autoantibodies (Aβ-autoantibodies) are currently investigated as potential diagnostic and therapeutic tools for Alzheimer’s disease (AD). In previous studies, their determination in serum and cerebrospinal fluid (CSF) using indirect ELISA has provided controversial results, which may be due to the presence of preformed Aβ antigen-antibody immune complexes. Based on the epitope specificity of the Aβ-autoantibodies, recently elucidated in our laboratory, we developed (a) a sandwich ELISA for the determination of circulating Aβ-IgG immune complexes and (b) an indirect ELISA for the determination of free Aβ-autoantibodies. This methodology was applied to the analysis of serum samples from healthy individuals within the age range of 18 to 89 years. Neuropsychological examination of the participants in this study indicated non-pathological, age-related cognitive decline, revealed especially by tests of visual memory and executive function, as well as speed-related tasks. The ELISA serum determinations showed significantly higher levels of Aβ-IgG immune complexes compared to free Aβ-autoantibodies, while no correlation with age or cognitive performance of the participants was found.

Zusammenfassung in einer weiteren Sprache

Fachgebiet (DDC)
540 Chemie

Schlagwörter

Konferenz

Rezension
undefined / . - undefined, undefined

Forschungsvorhaben

Organisationseinheiten

Zeitschriftenheft

Zugehörige Datensätze in KOPS

Zitieren

ISO 690MAFTEI, Madalina, Franka THURM, Vera Maria LEIRER, Christine A. F. von ARNIM, Thomas ELBERT, Michael PRZYBYLSKI, Iris-Tatjana KOLASSA, Marilena MANEA, 2012. Antigen-Bound and Free β-Amyloid Autoantibodies in Serum of Healthy Adults. In: PLoS ONE. 2012, 7(9), e44516. eISSN 1932-6203. Available under: doi: 10.1371/journal.pone.0044516
BibTex
@article{Maftei2012Antig-20613,
  year={2012},
  doi={10.1371/journal.pone.0044516},
  title={Antigen-Bound and Free β-Amyloid Autoantibodies in Serum of Healthy Adults},
  number={9},
  volume={7},
  journal={PLoS ONE},
  author={Maftei, Madalina and Thurm, Franka and Leirer, Vera Maria and Arnim, Christine A. F. von and Elbert, Thomas and Przybylski, Michael and Kolassa, Iris-Tatjana and Manea, Marilena},
  note={Article Number: e44516}
}
RDF
<rdf:RDF
    xmlns:dcterms="http://purl.org/dc/terms/"
    xmlns:dc="http://purl.org/dc/elements/1.1/"
    xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#"
    xmlns:bibo="http://purl.org/ontology/bibo/"
    xmlns:dspace="http://digital-repositories.org/ontologies/dspace/0.1.0#"
    xmlns:foaf="http://xmlns.com/foaf/0.1/"
    xmlns:void="http://rdfs.org/ns/void#"
    xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > 
  <rdf:Description rdf:about="https://kops.uni-konstanz.de/server/rdf/resource/123456789/20613">
    <dc:contributor>Manea, Marilena</dc:contributor>
    <dc:creator>Maftei, Madalina</dc:creator>
    <dc:contributor>Thurm, Franka</dc:contributor>
    <dcterms:isPartOf rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/52"/>
    <dcterms:rights rdf:resource="https://rightsstatements.org/page/InC/1.0/"/>
    <dspace:isPartOfCollection rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/52"/>
    <dcterms:issued>2012</dcterms:issued>
    <dc:contributor>Arnim, Christine A. F. von</dc:contributor>
    <dc:creator>Manea, Marilena</dc:creator>
    <dspace:hasBitstream rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/20613/2/maftei_etal_Antigen.pdf"/>
    <dcterms:hasPart rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/20613/2/maftei_etal_Antigen.pdf"/>
    <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2012-10-08T11:12:03Z</dcterms:available>
    <dc:creator>Thurm, Franka</dc:creator>
    <dcterms:title>Antigen-Bound and Free β-Amyloid Autoantibodies in Serum of Healthy Adults</dcterms:title>
    <dcterms:isPartOf rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/29"/>
    <dspace:isPartOfCollection rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/29"/>
    <dc:contributor>Leirer, Vera Maria</dc:contributor>
    <dc:rights>terms-of-use</dc:rights>
    <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2012-10-08T11:12:03Z</dc:date>
    <dcterms:bibliographicCitation>PLoS ONE ; 7 (2012), 9. - e44516</dcterms:bibliographicCitation>
    <void:sparqlEndpoint rdf:resource="http://localhost/fuseki/dspace/sparql"/>
    <dc:creator>Arnim, Christine A. F. von</dc:creator>
    <foaf:homepage rdf:resource="http://localhost:8080/"/>
    <dc:creator>Elbert, Thomas</dc:creator>
    <dc:contributor>Przybylski, Michael</dc:contributor>
    <dc:creator>Leirer, Vera Maria</dc:creator>
    <dcterms:abstract xml:lang="eng">Physiological β-amyloid autoantibodies (Aβ-autoantibodies) are currently investigated as potential diagnostic and therapeutic tools for Alzheimer’s disease (AD). In previous studies, their determination in serum and cerebrospinal fluid (CSF) using indirect ELISA has provided controversial results, which may be due to the presence of preformed Aβ antigen-antibody immune complexes. Based on the epitope specificity of the Aβ-autoantibodies, recently elucidated in our laboratory, we developed (a) a sandwich ELISA for the determination of circulating Aβ-IgG immune complexes and (b) an indirect ELISA for the determination of free Aβ-autoantibodies. This methodology was applied to the analysis of serum samples from healthy individuals within the age range of 18 to 89 years. Neuropsychological examination of the participants in this study indicated non-pathological, age-related cognitive decline, revealed especially by tests of visual memory and executive function, as well as speed-related tasks. The ELISA serum determinations showed significantly higher levels of Aβ-IgG immune complexes compared to free Aβ-autoantibodies, while no correlation with age or cognitive performance of the participants was found.</dcterms:abstract>
    <dc:contributor>Maftei, Madalina</dc:contributor>
    <dc:contributor>Kolassa, Iris-Tatjana</dc:contributor>
    <dc:contributor>Elbert, Thomas</dc:contributor>
    <dc:creator>Kolassa, Iris-Tatjana</dc:creator>
    <bibo:uri rdf:resource="http://kops.uni-konstanz.de/handle/123456789/20613"/>
    <dc:language>eng</dc:language>
    <dc:creator>Przybylski, Michael</dc:creator>
  </rdf:Description>
</rdf:RDF>

Interner Vermerk

xmlui.Submission.submit.DescribeStep.inputForms.label.kops_note_fromSubmitter

Kontakt
URL der Originalveröffentl.

Prüfdatum der URL

Prüfungsdatum der Dissertation

Finanzierungsart

Kommentar zur Publikation

Allianzlizenz
Corresponding Authors der Uni Konstanz vorhanden
Internationale Co-Autor:innen
Universitätsbibliographie
Ja
Begutachtet
Diese Publikation teilen