Immunolocalization of the exocytosis-sensitive phosphoprotein, PP63/parafusin, in Paramecium cells using antibodies against recombinant protein

Kurzanzeige
dc.contributor.authorKissmehl, Roland
dc.contributor.authorHauser, Karin
dc.contributor.authorGössringer, Markusdeu
dc.contributor.authorMomayezi, Massouddeu
dc.contributor.authorKlauke, Norbertdeu
dc.contributor.authorPlattner, Helmut
dc.date.accessioned2011-03-24T17:45:40Zdeu
dc.date.available2011-03-24T17:45:40Zdeu
dc.date.issued1998deu
dc.description.abstractWe have localized a structure-bound fraction of the exocytosis-sensitive phosphoprotein, PP63/parafusin (PP63/pf), in Paramecium cells by widely different methods. We combined cell fractionation, western blots, as well as light and electron microscopy (pre- and postembedding immunolabeling), applying antibodies against the recombinant protein. PP63/pf is considerably enriched in certain cortical structures, notably the outlines of regular surface fields (kinetids), docking sites of secretory organelles (trichocysts) and the membranes of subplasmalemmal Ca2+-stores (alveolar sacs). From our localization studies we tentatively derive several potential functions for PP63/pf, including cell surface structuring, assembly of exocytosis sites, and/or Ca2+ homeostasis.deu
dc.description.versionpublished
dc.format.mimetypeapplication/pdfdeu
dc.identifier.citationFirst publ. in: Histochemistry and Cell Biology ; 110 (1998), 1. - S. 1-8deu
dc.identifier.doi10.1007/s004180050258
dc.identifier.ppn273661442deu
dc.identifier.urihttp://kops.uni-konstanz.de/handle/123456789/8685
dc.language.isoengdeu
dc.legacy.dateIssued2007deu
dc.rightsAttribution-NonCommercial-NoDerivs 2.0 Generic
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/2.0/
dc.subject.ddc570deu
dc.titleImmunolocalization of the exocytosis-sensitive phosphoprotein, PP63/parafusin, in Paramecium cells using antibodies against recombinant proteineng
dc.typeJOURNAL_ARTICLEdeu
dspace.entity.typePublication
kops.citation.bibtex
@article{Kissmehl1998Immun-8685,
  year={1998},
  doi={10.1007/s004180050258},
  title={Immunolocalization of the exocytosis-sensitive phosphoprotein, PP63/parafusin, in Paramecium cells using antibodies against recombinant protein},
  number={1},
  volume={110},
  issn={0948-6143},
  journal={Histochemistry and Cell Biology},
  pages={1--8},
  author={Kissmehl, Roland and Hauser, Karin and Gössringer, Markus and Momayezi, Massoud and Klauke, Norbert and Plattner, Helmut}
}
kops.citation.iso690KISSMEHL, Roland, Karin HAUSER, Markus GÖSSRINGER, Massoud MOMAYEZI, Norbert KLAUKE, Helmut PLATTNER, 1998. Immunolocalization of the exocytosis-sensitive phosphoprotein, PP63/parafusin, in Paramecium cells using antibodies against recombinant protein. In: Histochemistry and Cell Biology. 1998, 110(1), pp. 1-8. ISSN 0948-6143. eISSN 1432-119X. Available under: doi: 10.1007/s004180050258deu
kops.citation.iso690KISSMEHL, Roland, Karin HAUSER, Markus GÖSSRINGER, Massoud MOMAYEZI, Norbert KLAUKE, Helmut PLATTNER, 1998. Immunolocalization of the exocytosis-sensitive phosphoprotein, PP63/parafusin, in Paramecium cells using antibodies against recombinant protein. In: Histochemistry and Cell Biology. 1998, 110(1), pp. 1-8. ISSN 0948-6143. eISSN 1432-119X. Available under: doi: 10.1007/s004180050258eng
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