Immunolocalization of the exocytosis-sensitive phosphoprotein, PP63/parafusin, in Paramecium cells using antibodies against recombinant protein

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Immunolocalization_of_the_exocytosis.pdf
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1998
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Gössringer, Markus
Momayezi, Massoud
Klauke, Norbert
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Histochemistry and Cell Biology. 1998, 110(1), pp. 1-8. ISSN 0948-6143. eISSN 1432-119X. Available under: doi: 10.1007/s004180050258
Zusammenfassung

We have localized a structure-bound fraction of the exocytosis-sensitive phosphoprotein, PP63/parafusin (PP63/pf), in Paramecium cells by widely different methods. We combined cell fractionation, western blots, as well as light and electron microscopy (pre- and postembedding immunolabeling), applying antibodies against the recombinant protein. PP63/pf is considerably enriched in certain cortical structures, notably the outlines of regular surface fields (kinetids), docking sites of secretory organelles (trichocysts) and the membranes of subplasmalemmal Ca2+-stores (alveolar sacs). From our localization studies we tentatively derive several potential functions for PP63/pf, including cell surface structuring, assembly of exocytosis sites, and/or Ca2+ homeostasis.

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570 Biowissenschaften, Biologie
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ISO 690KISSMEHL, Roland, Karin HAUSER, Markus GÖSSRINGER, Massoud MOMAYEZI, Norbert KLAUKE, Helmut PLATTNER, 1998. Immunolocalization of the exocytosis-sensitive phosphoprotein, PP63/parafusin, in Paramecium cells using antibodies against recombinant protein. In: Histochemistry and Cell Biology. 1998, 110(1), pp. 1-8. ISSN 0948-6143. eISSN 1432-119X. Available under: doi: 10.1007/s004180050258
BibTex
@article{Kissmehl1998Immun-8685,
  year={1998},
  doi={10.1007/s004180050258},
  title={Immunolocalization of the exocytosis-sensitive phosphoprotein, PP63/parafusin, in Paramecium cells using antibodies against recombinant protein},
  number={1},
  volume={110},
  issn={0948-6143},
  journal={Histochemistry and Cell Biology},
  pages={1--8},
  author={Kissmehl, Roland and Hauser, Karin and Gössringer, Markus and Momayezi, Massoud and Klauke, Norbert and Plattner, Helmut}
}
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