Cyclin B3 implements timely vertebrate oocyte arrest for fertilization

Lade...
Vorschaubild
Dateien
Zu diesem Dokument gibt es keine Dateien.
Datum
2022
Herausgeber:innen
Kontakt
ISSN der Zeitschrift
Electronic ISSN
ISBN
Bibliografische Daten
Verlag
Schriftenreihe
Auflagebezeichnung
URI (zitierfähiger Link)
ArXiv-ID
Internationale Patentnummer
EU-Projektnummer
DFG-Projektnummer
Forschungsförderung
Projekt
Open Access-Veröffentlichung
Sammlungen
Gesperrt bis
Titel in einer weiteren Sprache
Forschungsvorhaben
Organisationseinheiten
Zeitschriftenheft
Publikationstyp
Zeitschriftenartikel
Publikationsstatus
Published
Erschienen in
Developmental Cell. Cell Press. 2022, 57(19), pp. 2305-2320.e6. ISSN 1534-5807. eISSN 1878-1551. Available under: doi: 10.1016/j.devcel.2022.09.005
Zusammenfassung

To ensure successful offspring ploidy, vertebrate oocytes must halt the cell cycle in meiosis II until sperm entry. Emi2 is essential to keep oocytes arrested until fertilization. However, how this arrest is implemented exclusively in meiosis II and not prematurely in meiosis I has until now remained enigmatic. Using mouse and frog oocytes, we show here that cyclin B3, an understudied B-type cyclin, is essential to keep Emi2 levels low in meiosis I. Direct phosphorylation of Emi2 at an evolutionarily highly conserved site by Cdk1/cyclin B3 targets Emi2 for degradation. In contrast, Cdk1/cyclin B1 is inefficient in Emi2 phosphorylation, and this provides a molecular explanation for the requirement of different B-type cyclins for oocyte maturation. Cyclin B3 degradation at exit from meiosis I enables Emi2 accumulation and thus timely arrest in meiosis II. Our findings illuminate the evolutionarily conserved mechanisms that control oocyte arrest for fertilization at the correct cell-cycle stage, which is essential for embryo viability.

Zusammenfassung in einer weiteren Sprache
Fachgebiet (DDC)
570 Biowissenschaften, Biologie
Schlagwörter
meiosis, cyclin B3, Emi2/XErp1, CSF-arrest, oocytes, cell cycle control, cyclin specificity
Konferenz
Rezension
undefined / . - undefined, undefined
Zitieren
ISO 690BOUFTAS, Nora, Lena SCHNEIDER, Marc HALDER, Rebecca DEMMIG, Martina BAACK, Melanie WALTER, Hiba AL ABDALLAH, Patrick WEHRLE, Andreas HEIM, Katja WASSMANN, Thomas U. MAYER, 2022. Cyclin B3 implements timely vertebrate oocyte arrest for fertilization. In: Developmental Cell. Cell Press. 2022, 57(19), pp. 2305-2320.e6. ISSN 1534-5807. eISSN 1878-1551. Available under: doi: 10.1016/j.devcel.2022.09.005
BibTex
@article{Bouftas2022Cycli-59246,
  year={2022},
  doi={10.1016/j.devcel.2022.09.005},
  title={Cyclin B3 implements timely vertebrate oocyte arrest for fertilization},
  number={19},
  volume={57},
  issn={1534-5807},
  journal={Developmental Cell},
  pages={2305--2320.e6},
  author={Bouftas, Nora and Schneider, Lena and Halder, Marc and Demmig, Rebecca and Baack, Martina and Walter, Melanie and Al Abdallah, Hiba and Wehrle, Patrick and Heim, Andreas and Wassmann, Katja and Mayer, Thomas U.}
}
RDF
<rdf:RDF
    xmlns:dcterms="http://purl.org/dc/terms/"
    xmlns:dc="http://purl.org/dc/elements/1.1/"
    xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#"
    xmlns:bibo="http://purl.org/ontology/bibo/"
    xmlns:dspace="http://digital-repositories.org/ontologies/dspace/0.1.0#"
    xmlns:foaf="http://xmlns.com/foaf/0.1/"
    xmlns:void="http://rdfs.org/ns/void#"
    xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > 
  <rdf:Description rdf:about="https://kops.uni-konstanz.de/server/rdf/resource/123456789/59246">
    <dc:contributor>Walter, Melanie</dc:contributor>
    <foaf:homepage rdf:resource="http://localhost:8080/"/>
    <dc:language>eng</dc:language>
    <dc:creator>Wassmann, Katja</dc:creator>
    <void:sparqlEndpoint rdf:resource="http://localhost/fuseki/dspace/sparql"/>
    <dc:contributor>Demmig, Rebecca</dc:contributor>
    <bibo:uri rdf:resource="https://kops.uni-konstanz.de/handle/123456789/59246"/>
    <dc:contributor>Schneider, Lena</dc:contributor>
    <dc:creator>Baack, Martina</dc:creator>
    <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2022-11-22T15:57:08Z</dc:date>
    <dc:contributor>Al Abdallah, Hiba</dc:contributor>
    <dcterms:abstract xml:lang="eng">To ensure successful offspring ploidy, vertebrate oocytes must halt the cell cycle in meiosis II until sperm entry. Emi2 is essential to keep oocytes arrested until fertilization. However, how this arrest is implemented exclusively in meiosis II and not prematurely in meiosis I has until now remained enigmatic. Using mouse and frog oocytes, we show here that cyclin B3, an understudied B-type cyclin, is essential to keep Emi2 levels low in meiosis I. Direct phosphorylation of Emi2 at an evolutionarily highly conserved site by Cdk1/cyclin B3 targets Emi2 for degradation. In contrast, Cdk1/cyclin B1 is inefficient in Emi2 phosphorylation, and this provides a molecular explanation for the requirement of different B-type cyclins for oocyte maturation. Cyclin B3 degradation at exit from meiosis I enables Emi2 accumulation and thus timely arrest in meiosis II. Our findings illuminate the evolutionarily conserved mechanisms that control oocyte arrest for fertilization at the correct cell-cycle stage, which is essential for embryo viability.</dcterms:abstract>
    <dspace:isPartOfCollection rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/28"/>
    <dc:creator>Demmig, Rebecca</dc:creator>
    <dc:creator>Heim, Andreas</dc:creator>
    <dc:creator>Schneider, Lena</dc:creator>
    <dc:contributor>Wehrle, Patrick</dc:contributor>
    <dc:creator>Al Abdallah, Hiba</dc:creator>
    <dc:creator>Walter, Melanie</dc:creator>
    <dc:contributor>Wassmann, Katja</dc:contributor>
    <dc:rights>terms-of-use</dc:rights>
    <dc:creator>Mayer, Thomas U.</dc:creator>
    <dcterms:rights rdf:resource="https://rightsstatements.org/page/InC/1.0/"/>
    <dcterms:isPartOf rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/28"/>
    <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2022-11-22T15:57:08Z</dcterms:available>
    <dc:contributor>Halder, Marc</dc:contributor>
    <dc:creator>Wehrle, Patrick</dc:creator>
    <dc:creator>Bouftas, Nora</dc:creator>
    <dc:creator>Halder, Marc</dc:creator>
    <dc:contributor>Bouftas, Nora</dc:contributor>
    <dc:contributor>Baack, Martina</dc:contributor>
    <dc:contributor>Mayer, Thomas U.</dc:contributor>
    <dc:contributor>Heim, Andreas</dc:contributor>
    <dcterms:title>Cyclin B3 implements timely vertebrate oocyte arrest for fertilization</dcterms:title>
    <dcterms:issued>2022</dcterms:issued>
  </rdf:Description>
</rdf:RDF>
Interner Vermerk
xmlui.Submission.submit.DescribeStep.inputForms.label.kops_note_fromSubmitter
Kontakt
URL der Originalveröffentl.
Prüfdatum der URL
Prüfungsdatum der Dissertation
Finanzierungsart
Kommentar zur Publikation
Allianzlizenz
Corresponding Authors der Uni Konstanz vorhanden
Internationale Co-Autor:innen
Universitätsbibliographie
Ja
Begutachtet
Ja