Cyclin B3 implements timely vertebrate oocyte arrest for fertilization

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2022
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To ensure successful offspring ploidy, vertebrate oocytes must halt the cell cycle in meiosis II until sperm entry. Emi2 is essential to keep oocytes arrested until fertilization. However, how this arrest is implemented exclusively in meiosis II and not prematurely in meiosis I has until now remained enigmatic. Using mouse and frog oocytes, we show here that cyclin B3, an understudied B-type cyclin, is essential to keep Emi2 levels low in meiosis I. Direct phosphorylation of Emi2 at an evolutionarily highly conserved site by Cdk1/cyclin B3 targets Emi2 for degradation. In contrast, Cdk1/cyclin B1 is inefficient in Emi2 phosphorylation, and this provides a molecular explanation for the requirement of different B-type cyclins for oocyte maturation. Cyclin B3 degradation at exit from meiosis I enables Emi2 accumulation and thus timely arrest in meiosis II. Our findings illuminate the evolutionarily conserved mechanisms that control oocyte arrest for fertilization at the correct cell-cycle stage, which is essential for embryo viability.

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570 Biowissenschaften, Biologie
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meiosis, cyclin B3, Emi2/XErp1, CSF-arrest, oocytes, cell cycle control, cyclin specificity
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ISO 690BOUFTAS, Nora, Lena SCHNEIDER, Marc HALDER, Rebecca DEMMIG, Martina BAACK, Melanie WALTER, Hiba AL ABDALLAH, Patrick WEHRLE, Andreas HEIM, Katja WASSMANN, Thomas U. MAYER, 2022. Cyclin B3 implements timely vertebrate oocyte arrest for fertilization. In: Developmental Cell. Cell Press. 2022, 57(19), pp. 2305-2320.e6. ISSN 1534-5807. eISSN 1878-1551. Available under: doi: 10.1016/j.devcel.2022.09.005
BibTex
@article{Bouftas2022Cycli-59246,
  year={2022},
  doi={10.1016/j.devcel.2022.09.005},
  title={Cyclin B3 implements timely vertebrate oocyte arrest for fertilization},
  number={19},
  volume={57},
  issn={1534-5807},
  journal={Developmental Cell},
  pages={2305--2320.e6},
  author={Bouftas, Nora and Schneider, Lena and Halder, Marc and Demmig, Rebecca and Baack, Martina and Walter, Melanie and Al Abdallah, Hiba and Wehrle, Patrick and Heim, Andreas and Wassmann, Katja and Mayer, Thomas U.}
}
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