Total-internal-reflection fluorescence microscopy with W-shaped axicon mirrors

Kurzanzeige
dc.contributor.authorLei, Ming
dc.contributor.authorZumbusch, Andreas
dc.date.accessioned2011-03-24T18:13:32Zdeu
dc.date.available2011-03-24T18:13:32Zdeu
dc.date.issued2010deu
dc.description.abstractA scheme based on a W-shaped axicon mirror device for total-internal-reflection fluorescence microscopy (TIRFM)is presented. This approach combines the advantages of higher efficiency compared with traditional TIRFM, adjustable illumination area, and simple switching between wide-field and TIRF imaging modes. TIRF images obtained with this approach are free of shadow artifacts and of interference fringes. Example micrographs of fluorescently labeled polystyrene beads, of Convallaria majalis tissue, and of Propidium-iodide-labeled Chinese hamster ovary cells are shown, and the capabilities of the scheme are discussed.eng
dc.description.versionpublished
dc.format.mimetypeapplication/pdfdeu
dc.identifier.citationFirst publ. in: Optics Letters 35 (2010), 23, pp. 4057-4059deu
dc.identifier.doi10.1364/OL.35.004057
dc.identifier.pmid21124611
dc.identifier.ppn334531152deu
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dc.language.isoengdeu
dc.legacy.dateIssued2010deu
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dc.subject.ddc540deu
dc.titleTotal-internal-reflection fluorescence microscopy with W-shaped axicon mirrorseng
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  volume={35},
  issn={0146-9592},
  journal={Optics Letters},
  pages={4057--4059},
  author={Lei, Ming and Zumbusch, Andreas}
}
kops.citation.iso690LEI, Ming, Andreas ZUMBUSCH, 2010. Total-internal-reflection fluorescence microscopy with W-shaped axicon mirrors. In: Optics Letters. 2010, 35(23), pp. 4057-4059. ISSN 0146-9592. eISSN 1539-4794. Available under: doi: 10.1364/OL.35.004057deu
kops.citation.iso690LEI, Ming, Andreas ZUMBUSCH, 2010. Total-internal-reflection fluorescence microscopy with W-shaped axicon mirrors. In: Optics Letters. 2010, 35(23), pp. 4057-4059. ISSN 0146-9592. eISSN 1539-4794. Available under: doi: 10.1364/OL.35.004057eng
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