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Total-internal-reflection fluorescence microscopy with W-shaped axicon mirrors

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2010

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http://nbn-resolving.de/urn:nbn:de:bsz:352-opus-125820
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https://doi.org/10.1364/OL.35.004057
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Optics Letters. 2010, 35(23), pp. 4057-4059. ISSN 0146-9592. eISSN 1539-4794. Available under: doi: 10.1364/OL.35.004057

Zusammenfassung

A scheme based on a W-shaped axicon mirror device for total-internal-reflection fluorescence microscopy (TIRFM)is presented. This approach combines the advantages of higher efficiency compared with traditional TIRFM, adjustable illumination area, and simple switching between wide-field and TIRF imaging modes. TIRF images obtained with this approach are free of shadow artifacts and of interference fringes. Example micrographs of fluorescently labeled polystyrene beads, of Convallaria majalis tissue, and of Propidium-iodide-labeled Chinese hamster ovary cells are shown, and the capabilities of the scheme are discussed.

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540 Chemie

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ISO 690LEI, Ming, Andreas ZUMBUSCH, 2010. Total-internal-reflection fluorescence microscopy with W-shaped axicon mirrors. In: Optics Letters. 2010, 35(23), pp. 4057-4059. ISSN 0146-9592. eISSN 1539-4794. Available under: doi: 10.1364/OL.35.004057
BibTex
@article{Lei2010Total-9663,
  year={2010},
  doi={10.1364/OL.35.004057},
  title={Total-internal-reflection fluorescence microscopy with W-shaped axicon mirrors},
  number={23},
  volume={35},
  issn={0146-9592},
  journal={Optics Letters},
  pages={4057--4059},
  author={Lei, Ming and Zumbusch, Andreas}
}
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