Programmable and Highly Resolved In Vitro Detection of 5-Methylcytosine by TALEs

Kurzanzeige
dc.contributor.authorKubik, Grzegorz
dc.contributor.authorSchmidt, Moritz J.
dc.contributor.authorPenner, Johanna E.
dc.contributor.authorSummerer, Daniel
dc.date.accessioned2015-02-20T09:02:18Z
dc.date.available2015-02-20T09:02:18Z
dc.date.issued2014eng
dc.description.abstractGene expression is extensively regulated by specific patterns of genomic 5-methylcytosine (mC), but the ability to directly detect this modification at user-defined genomic loci is limited. One reason is the lack of molecules that discriminate between mC and cytosine (C) and at the same time provide inherent, programmable sequence-selectivity. Programmable transcription-activator-like effectors (TALEs) have been observed to exhibit mC-sensitivity in vivo, but to only a limited extent in vitro. We report an mC-detection assay based on TALE control of DNA replication that displays unexpectedly strong mC-discrimination ability in vitro. The status and level of mC modification at single positions in oligonucleotides can be determined unambiguously by this assay, independently of the overall target sequence. Moreover, discrimination is reliably observed for positions bound by N-terminal and central regions of TALEs. This indicates the wide scope and robustness of the approach for highly resolved mC detection and enabled the detection of a single mC in a large, eukaryotic genome.eng
dc.description.versionpublished
dc.identifier.doi10.1002/anie.201400436eng
dc.identifier.urihttp://kops.uni-konstanz.de/handle/123456789/29926
dc.language.isoengeng
dc.subjectepigenetics, 5-methylcytosine, programmable protein scaffolds, protein-DNA interactions, TAL effectorseng
dc.subject.ddc540eng
dc.titleProgrammable and Highly Resolved In Vitro Detection of 5-Methylcytosine by TALEseng
dc.typeJOURNAL_ARTICLEeng
dspace.entity.typePublication
kops.citation.bibtex
@article{Kubik2014Progr-29926,
  year={2014},
  doi={10.1002/anie.201400436},
  title={Programmable and Highly Resolved In Vitro Detection of 5-Methylcytosine by TALEs},
  number={23},
  volume={53},
  issn={0570-0833},
  journal={Angewandte Chemie International Edition},
  pages={6002--6006},
  author={Kubik, Grzegorz and Schmidt, Moritz J. and Penner, Johanna E. and Summerer, Daniel}
}
kops.citation.iso690KUBIK, Grzegorz, Moritz J. SCHMIDT, Johanna E. PENNER, Daniel SUMMERER, 2014. Programmable and Highly Resolved In Vitro Detection of 5-Methylcytosine by TALEs. In: Angewandte Chemie International Edition. 2014, 53(23), pp. 6002-6006. ISSN 0570-0833. eISSN 1521-3773. Available under: doi: 10.1002/anie.201400436deu
kops.citation.iso690KUBIK, Grzegorz, Moritz J. SCHMIDT, Johanna E. PENNER, Daniel SUMMERER, 2014. Programmable and Highly Resolved In Vitro Detection of 5-Methylcytosine by TALEs. In: Angewandte Chemie International Edition. 2014, 53(23), pp. 6002-6006. ISSN 0570-0833. eISSN 1521-3773. Available under: doi: 10.1002/anie.201400436eng
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temp.internal.duplicates<p>Keine Dubletten gefunden. Letzte Überprüfung: 26.11.2014 16:45:04</p>deu

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