Programmable and Highly Resolved In Vitro Detection of 5-Methylcytosine by TALEs

Kubik, Grzegorz; Schmidt, Moritz J.; Penner, Johanna E.; Summerer, Daniel

Programmable and Highly Resolved In Vitro Detection of 5-Methylcytosine by TALEs

Dateien

Zu diesem Dokument gibt es keine Dateien.

Datum

2014

Autor:innen

Kubik, Grzegorz

Schmidt, Moritz J.

Penner, Johanna E.

Summerer, Daniel

DOI (zitierfähiger Link)

https://doi.org/10.1002/anie.201400436

Sammlungen

Chemie
Zukunftskolleg

Publikationstyp

Zeitschriftenartikel

unikn.publication.listelement.citation.prefix.version.undefined

Angewandte Chemie International Edition. 2014, 53(23), pp. 6002-6006. ISSN 0570-0833. eISSN 1521-3773. Available under: doi: 10.1002/anie.201400436

Zusammenfassung

Gene expression is extensively regulated by specific patterns of genomic 5-methylcytosine (mC), but the ability to directly detect this modification at user-defined genomic loci is limited. One reason is the lack of molecules that discriminate between mC and cytosine (C) and at the same time provide inherent, programmable sequence-selectivity. Programmable transcription-activator-like effectors (TALEs) have been observed to exhibit mC-sensitivity in vivo, but to only a limited extent in vitro. We report an mC-detection assay based on TALE control of DNA replication that displays unexpectedly strong mC-discrimination ability in vitro. The status and level of mC modification at single positions in oligonucleotides can be determined unambiguously by this assay, independently of the overall target sequence. Moreover, discrimination is reliably observed for positions bound by N-terminal and central regions of TALEs. This indicates the wide scope and robustness of the approach for highly resolved mC detection and enabled the detection of a single mC in a large, eukaryotic genome.

Fachgebiet (DDC)

540 Chemie

Schlagwörter

epigenetics, 5-methylcytosine, programmable protein scaffolds, protein-DNA interactions, TAL effectors

Zitieren

ISO 690

KUBIK, Grzegorz, Moritz J. SCHMIDT, Johanna E. PENNER, Daniel SUMMERER, 2014. Programmable and Highly Resolved In Vitro Detection of 5-Methylcytosine by TALEs. In: Angewandte Chemie International Edition. 2014, 53(23), pp. 6002-6006. ISSN 0570-0833. eISSN 1521-3773. Available under: doi: 10.1002/anie.201400436

BibTex

@article{Kubik2014Progr-29926,
  year={2014},
  doi={10.1002/anie.201400436},
  title={Programmable and Highly Resolved In Vitro Detection of 5-Methylcytosine by TALEs},
  number={23},
  volume={53},
  issn={0570-0833},
  journal={Angewandte Chemie International Edition},
  pages={6002--6006},
  author={Kubik, Grzegorz and Schmidt, Moritz J. and Penner, Johanna E. and Summerer, Daniel}
}

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