Freeze etching of cells without cryoprotectants

Kurzanzeige
dc.contributor.authorPlattner, Helmut
dc.contributor.authorFischer, Walter M.deu
dc.contributor.authorSchmitt, Werner W.deu
dc.contributor.authorBachmann, Luisdeu
dc.date.accessioned2011-03-24T17:33:02Zdeu
dc.date.available2011-03-24T17:33:02Zdeu
dc.date.issued1972deu
dc.description.abstractThe technique of spray-freeze etching was applied to unicellular organisms. The superior freezing rates obtainable with this method gave excellent cryofixation on Chlorella, Euglena, and spermatozoa without the use of antifreeze agents, and cell damage due to ice crystal formation was never observed. In many instances the resultant morphology differed significantly from that obtained from glycerol-treated, freeze-etched cells. Furthermore, viability studies of spray-frozen Chlorella compared favorably with cells frozen by other methods.eng
dc.description.versionpublished
dc.format.mimetypeapplication/pdfdeu
dc.identifier.citationFirst publ. in: Journal of Cell Biology 53 (1972), pp. 116-126deu
dc.identifier.doi10.1083/jcb.53.1.116
dc.identifier.ppn275043428deu
dc.identifier.urihttp://kops.uni-konstanz.de/handle/123456789/7259
dc.language.isoengdeu
dc.legacy.dateIssued2007deu
dc.rightsAttribution-NonCommercial-NoDerivs 2.0 Generic
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/2.0/
dc.subject.ddc570deu
dc.titleFreeze etching of cells without cryoprotectantseng
dc.typeJOURNAL_ARTICLEdeu
dspace.entity.typePublication
kops.citation.bibtex
@article{Plattner1972Freez-7259,
  year={1972},
  doi={10.1083/jcb.53.1.116},
  title={Freeze etching of cells without cryoprotectants},
  number={1},
  volume={53},
  issn={0021-9525},
  journal={Journal of Cell Biology},
  pages={116--126},
  author={Plattner, Helmut and Fischer, Walter M. and Schmitt, Werner W. and Bachmann, Luis}
}
kops.citation.iso690PLATTNER, Helmut, Walter M. FISCHER, Werner W. SCHMITT, Luis BACHMANN, 1972. Freeze etching of cells without cryoprotectants. In: Journal of Cell Biology. 1972, 53(1), pp. 116-126. ISSN 0021-9525. eISSN 1540-8140. Available under: doi: 10.1083/jcb.53.1.116deu
kops.citation.iso690PLATTNER, Helmut, Walter M. FISCHER, Werner W. SCHMITT, Luis BACHMANN, 1972. Freeze etching of cells without cryoprotectants. In: Journal of Cell Biology. 1972, 53(1), pp. 116-126. ISSN 0021-9525. eISSN 1540-8140. Available under: doi: 10.1083/jcb.53.1.116eng
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