Freeze etching of cells without cryoprotectants

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1972
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Fischer, Walter M.
Schmitt, Werner W.
Bachmann, Luis
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Journal of Cell Biology. 1972, 53(1), pp. 116-126. ISSN 0021-9525. eISSN 1540-8140. Available under: doi: 10.1083/jcb.53.1.116
Zusammenfassung

The technique of spray-freeze etching was applied to unicellular organisms. The superior freezing rates obtainable with this method gave excellent cryofixation on Chlorella, Euglena, and spermatozoa without the use of antifreeze agents, and cell damage due to ice crystal formation was never observed. In many instances the resultant morphology differed significantly from that obtained from glycerol-treated, freeze-etched cells. Furthermore, viability studies of spray-frozen Chlorella compared favorably with cells frozen by other methods.

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570 Biowissenschaften, Biologie
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ISO 690PLATTNER, Helmut, Walter M. FISCHER, Werner W. SCHMITT, Luis BACHMANN, 1972. Freeze etching of cells without cryoprotectants. In: Journal of Cell Biology. 1972, 53(1), pp. 116-126. ISSN 0021-9525. eISSN 1540-8140. Available under: doi: 10.1083/jcb.53.1.116
BibTex
@article{Plattner1972Freez-7259,
  year={1972},
  doi={10.1083/jcb.53.1.116},
  title={Freeze etching of cells without cryoprotectants},
  number={1},
  volume={53},
  issn={0021-9525},
  journal={Journal of Cell Biology},
  pages={116--126},
  author={Plattner, Helmut and Fischer, Walter M. and Schmitt, Werner W. and Bachmann, Luis}
}
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