@article{Deuerling1995Bacil-8093,
  year={1995},
  title={The ftsH gene of Bacillus subtilis is transiently induced after osmotic and temperature upshift},
  number={14},
  volume={177},
  journal={Journal of Bacteriology},
  pages={4105--4112},
  author={Deuerling, Elke and Paeslack, Bettina and Schumann, Wolfgang}
}

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    <dcterms:abstract xml:lang="eng">The ftsH gene of Bacillus subtilis has been identified as a salt-sensitive insertion mutation in strain UG1. Here, we show that UG1 has an insertion near the 3 end of ftsH. The salt sensitivity of this mutant was caused by reduction of ftsH mRNA levels by the synthesis of an artificial antisense RNA originating at a promoter located within the insertion and reading backwards into the ftsH gene. The salt-sensitive phenotype could be overcome by deleting the promoter from which the antisense RNA was transcribed. A physiological analysis of the isogenic wild-type strain in minimal medium revealed unimpaired growth at up to 1 M NaCl, and growth above 1.2 M NaCl was observed only after addition of the osmoprotectant proline or glycine betaine. In contrast, growth of strain UG1 was reduced at a salt concentration above 0.2 M, which could be rescued by the two compatible solutes already mentioned and also by trehalose. Primer extension revealed one potential transcription start site downstream of a putative vegetative promoter, which was activated after osmotic or temperature upshift. Northern (RNA blot) experiments led to the detection of a 2.1-kb transcript, suggesting that ftsH is monocistronic. A transcriptional fusion between ftsH and the gus reporter gene exhibited a twofold increase in b-glucuronidase activity after osmotic upshift. To further confirm the need for an enhanced level of FtsH protein after osmotic upshift, the promoter was replaced by the sucrose-inducible promoter PsacB. Whereas this mutant strain could grow in the absence of inducer in LB medium, it stopped growth immediately after addition of 1.1 M NaCl. We conclude that an increased amount of FtsH protein is essential for B. subtilis to cope with an increase in osmolarity or temperature.</dcterms:abstract>
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    <dcterms:bibliographicCitation>First publ. in: Journal of Bacteriology 177 (1995), 14, pp. 4105-4112</dcterms:bibliographicCitation>
    <dc:contributor>Deuerling, Elke</dc:contributor>
    <dcterms:issued>1995</dcterms:issued>
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    <dc:contributor>Schumann, Wolfgang</dc:contributor>
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    <dc:creator>Deuerling, Elke</dc:creator>
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    <dcterms:title>The ftsH gene of Bacillus subtilis is transiently induced after osmotic and temperature upshift</dcterms:title>
    <dc:creator>Schumann, Wolfgang</dc:creator>
    <dc:creator>Paeslack, Bettina</dc:creator>
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    <dc:contributor>Paeslack, Bettina</dc:contributor>
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