Enzyme characteristics of recombinant poly(ADP-ribose) polymerases-1 of rat and human origin mirror the correlation between cellular poly(ADP-ribosyl)ation capacity and species-specific life span

Kurzanzeige
dc.contributor.authorBeneke, Sascha
dc.contributor.authorScherr, Anna-Lena
dc.contributor.authorPonath, Viviane
dc.contributor.authorPopp, Oliver
dc.contributor.authorBürkle, Alexander
dc.date.accessioned2011-07-01T11:32:12Zdeu
dc.date.available2011-07-01T11:32:12Zdeu
dc.date.issued2010-05
dc.description.abstractPoly(ADP-ribosyl)ation is a posttranslational modification, which is involved in many cellular functions, including DNA repair and maintenance of genomic stability, and has also been implicated in cellular and organismal ageing. We have previously reported that maximum poly(ADP-ribosyl)ation capacity in mononuclear blood cells is correlated with mammalian life span. Here we show that the difference between a long-lived and a short-lived species tested (i.e. man and rat) is directly mirrored by the enzymatic parameters of recombinant poly(ADP-ribose) polymerase-1 (PARP-1), i.e. substrate affinity and reaction velocity. In addition, we have characterized two human PARP-1 alleles and assign their activity difference to their respective initial velocity and not substrate affinity.eng
dc.description.versionpublished
dc.format.mimetypeapplication/pdfdeu
dc.identifier.citationFirst publ. in: Mechanisms of Ageing and Development 131 (2010), 5, pp. 366-369deu
dc.identifier.doi10.1016/j.mad.2010.04.003deu
dc.identifier.pmid20399804
dc.identifier.ppn346742048deu
dc.identifier.urihttp://kops.uni-konstanz.de/handle/123456789/431
dc.language.isoengdeu
dc.legacy.dateIssued2011deu
dc.rightsterms-of-usedeu
dc.rights.urihttps://rightsstatements.org/page/InC/1.0/deu
dc.subjectPoly(ADP-ribose)polymerase-1deu
dc.subjectEnzyme activitydeu
dc.subjectLife spandeu
dc.subjectAlleledeu
dc.subject.ddc570deu
dc.titleEnzyme characteristics of recombinant poly(ADP-ribose) polymerases-1 of rat and human origin mirror the correlation between cellular poly(ADP-ribosyl)ation capacity and species-specific life spaneng
dc.typeJOURNAL_ARTICLEdeu
dspace.entity.typePublication
kops.citation.bibtex
@article{Beneke2010-05Enzym-431,
  year={2010},
  doi={10.1016/j.mad.2010.04.003},
  title={Enzyme characteristics of recombinant poly(ADP-ribose) polymerases-1 of rat and human origin mirror the correlation between cellular poly(ADP-ribosyl)ation capacity and species-specific life span},
  number={5},
  volume={131},
  issn={0047-6374},
  journal={Mechanisms of Ageing and Development},
  pages={366--369},
  author={Beneke, Sascha and Scherr, Anna-Lena and Ponath, Viviane and Popp, Oliver and Bürkle, Alexander}
}
kops.citation.iso690BENEKE, Sascha, Anna-Lena SCHERR, Viviane PONATH, Oliver POPP, Alexander BÜRKLE, 2010. Enzyme characteristics of recombinant poly(ADP-ribose) polymerases-1 of rat and human origin mirror the correlation between cellular poly(ADP-ribosyl)ation capacity and species-specific life span. In: Mechanisms of Ageing and Development. 2010, 131(5), pp. 366-369. ISSN 0047-6374. eISSN 1872-6216. Available under: doi: 10.1016/j.mad.2010.04.003deu
kops.citation.iso690BENEKE, Sascha, Anna-Lena SCHERR, Viviane PONATH, Oliver POPP, Alexander BÜRKLE, 2010. Enzyme characteristics of recombinant poly(ADP-ribose) polymerases-1 of rat and human origin mirror the correlation between cellular poly(ADP-ribosyl)ation capacity and species-specific life span. In: Mechanisms of Ageing and Development. 2010, 131(5), pp. 366-369. ISSN 0047-6374. eISSN 1872-6216. Available under: doi: 10.1016/j.mad.2010.04.003eng
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