Enzyme characteristics of recombinant poly(ADP-ribose) polymerases-1 of rat and human origin mirror the correlation between cellular poly(ADP-ribosyl)ation capacity and species-specific life span

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2010
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Zusammenfassung

Poly(ADP-ribosyl)ation is a posttranslational modification, which is involved in many cellular functions, including DNA repair and maintenance of genomic stability, and has also been implicated in cellular and organismal ageing. We have previously reported that maximum poly(ADP-ribosyl)ation capacity in mononuclear blood cells is correlated with mammalian life span. Here we show that the difference between a long-lived and a short-lived species tested (i.e. man and rat) is directly mirrored by the enzymatic parameters of recombinant poly(ADP-ribose) polymerase-1 (PARP-1), i.e. substrate affinity and reaction velocity. In addition, we have characterized two human PARP-1 alleles and assign their activity difference to their respective initial velocity and not substrate affinity.

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570 Biowissenschaften, Biologie
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Poly(ADP-ribose)polymerase-1, Enzyme activity, Life span, Allele
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ISO 690BENEKE, Sascha, Anna-Lena SCHERR, Viviane PONATH, Oliver POPP, Alexander BÜRKLE, 2010. Enzyme characteristics of recombinant poly(ADP-ribose) polymerases-1 of rat and human origin mirror the correlation between cellular poly(ADP-ribosyl)ation capacity and species-specific life span. In: Mechanisms of Ageing and Development. 2010, 131(5), pp. 366-369. ISSN 0047-6374. eISSN 1872-6216. Available under: doi: 10.1016/j.mad.2010.04.003
BibTex
@article{Beneke2010-05Enzym-431,
  year={2010},
  doi={10.1016/j.mad.2010.04.003},
  title={Enzyme characteristics of recombinant poly(ADP-ribose) polymerases-1 of rat and human origin mirror the correlation between cellular poly(ADP-ribosyl)ation capacity and species-specific life span},
  number={5},
  volume={131},
  issn={0047-6374},
  journal={Mechanisms of Ageing and Development},
  pages={366--369},
  author={Beneke, Sascha and Scherr, Anna-Lena and Ponath, Viviane and Popp, Oliver and Bürkle, Alexander}
}
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