Assembly of a protein “brush” by end-grafting titin fragments to liposomes
| dc.contributor.author | Chami, Mohamed | |
| dc.contributor.author | Franke, Barbara | |
| dc.contributor.author | Mayans, Olga | |
| dc.contributor.author | Kreplak, Laurent | |
| dc.date.accessioned | 2017-05-02T08:32:39Z | |
| dc.date.available | 2017-05-02T08:32:39Z | |
| dc.date.issued | 2011-08 | eng |
| dc.description.abstract | Titin is a giant filamentous protein of striated muscle composed of >300 immunoglobulin-like domains linked in tandem. Here, we demonstrate that a six-immunoglobulin fragment of titin carrying a poly-histidine tag forms a protein "brush" on liposomes containing metallochelating lipids. These specimens might allow the direct visualization by cryo-EM of frozen hydrated and unstained titin chains with preserved architectural features. | eng |
| dc.description.version | published | eng |
| dc.identifier.doi | 10.1016/j.jbiosc.2011.04.014 | eng |
| dc.identifier.pmid | 21616710 | eng |
| dc.identifier.uri | https://kops.uni-konstanz.de/handle/123456789/38668 | |
| dc.language.iso | eng | eng |
| dc.subject | Decorated liposomes; Titin; Cryo-electron microscopy; His-Tag; Nickel chelating lipid | eng |
| dc.subject.ddc | 570 | eng |
| dc.title | Assembly of a protein “brush” by end-grafting titin fragments to liposomes | eng |
| dc.type | JOURNAL_ARTICLE | eng |
| dspace.entity.type | Publication | |
| kops.citation.bibtex | @article{Chami2011-08Assem-38668,
year={2011},
doi={10.1016/j.jbiosc.2011.04.014},
title={Assembly of a protein “brush” by end-grafting titin fragments to liposomes},
number={2},
volume={112},
issn={1389-1723},
journal={Journal of Bioscience and Bioengineering},
pages={178--179},
author={Chami, Mohamed and Franke, Barbara and Mayans, Olga and Kreplak, Laurent}
} | |
| kops.citation.iso690 | CHAMI, Mohamed, Barbara FRANKE, Olga MAYANS, Laurent KREPLAK, 2011. Assembly of a protein “brush” by end-grafting titin fragments to liposomes. In: Journal of Bioscience and Bioengineering. 2011, 112(2), pp. 178-179. ISSN 1389-1723. eISSN 1347-4421. Available under: doi: 10.1016/j.jbiosc.2011.04.014 | deu |
| kops.citation.iso690 | CHAMI, Mohamed, Barbara FRANKE, Olga MAYANS, Laurent KREPLAK, 2011. Assembly of a protein “brush” by end-grafting titin fragments to liposomes. In: Journal of Bioscience and Bioengineering. 2011, 112(2), pp. 178-179. ISSN 1389-1723. eISSN 1347-4421. Available under: doi: 10.1016/j.jbiosc.2011.04.014 | eng |
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| kops.sourcefield.plain | Journal of Bioscience and Bioengineering. 2011, 112(2), pp. 178-179. ISSN 1389-1723. eISSN 1347-4421. Available under: doi: 10.1016/j.jbiosc.2011.04.014 | eng |
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