Assembly of a protein “brush” by end-grafting titin fragments to liposomes

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2011
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Chami, Mohamed
Kreplak, Laurent
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Zusammenfassung

Titin is a giant filamentous protein of striated muscle composed of >300 immunoglobulin-like domains linked in tandem. Here, we demonstrate that a six-immunoglobulin fragment of titin carrying a poly-histidine tag forms a protein "brush" on liposomes containing metallochelating lipids. These specimens might allow the direct visualization by cryo-EM of frozen hydrated and unstained titin chains with preserved architectural features.

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570 Biowissenschaften, Biologie
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Decorated liposomes; Titin; Cryo-electron microscopy; His-Tag; Nickel chelating lipid
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ISO 690CHAMI, Mohamed, Barbara FRANKE, Olga MAYANS, Laurent KREPLAK, 2011. Assembly of a protein “brush” by end-grafting titin fragments to liposomes. In: Journal of Bioscience and Bioengineering. 2011, 112(2), pp. 178-179. ISSN 1389-1723. eISSN 1347-4421. Available under: doi: 10.1016/j.jbiosc.2011.04.014
BibTex
@article{Chami2011-08Assem-38668,
  year={2011},
  doi={10.1016/j.jbiosc.2011.04.014},
  title={Assembly of a protein “brush” by end-grafting titin fragments to liposomes},
  number={2},
  volume={112},
  issn={1389-1723},
  journal={Journal of Bioscience and Bioengineering},
  pages={178--179},
  author={Chami, Mohamed and Franke, Barbara and Mayans, Olga and Kreplak, Laurent}
}
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