Assembly of a protein “brush” by end-grafting titin fragments to liposomes
Assembly of a protein “brush” by end-grafting titin fragments to liposomes
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2011
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Journal of Bioscience and Bioengineering ; 112 (2011), 2. - pp. 178-179. - ISSN 1389-1723. - eISSN 1347-4421
Abstract
Titin is a giant filamentous protein of striated muscle composed of >300 immunoglobulin-like domains linked in tandem. Here, we demonstrate that a six-immunoglobulin fragment of titin carrying a poly-histidine tag forms a protein "brush" on liposomes containing metallochelating lipids. These specimens might allow the direct visualization by cryo-EM of frozen hydrated and unstained titin chains with preserved architectural features.
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570 Biosciences, Biology
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Decorated liposomes; Titin; Cryo-electron microscopy; His-Tag; Nickel chelating lipid
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CHAMI, Mohamed, Barbara FRANKE, Olga MAYANS, Laurent KREPLAK, 2011. Assembly of a protein “brush” by end-grafting titin fragments to liposomes. In: Journal of Bioscience and Bioengineering. 112(2), pp. 178-179. ISSN 1389-1723. eISSN 1347-4421. Available under: doi: 10.1016/j.jbiosc.2011.04.014BibTex
@article{Chami2011-08Assem-38668, year={2011}, doi={10.1016/j.jbiosc.2011.04.014}, title={Assembly of a protein “brush” by end-grafting titin fragments to liposomes}, number={2}, volume={112}, issn={1389-1723}, journal={Journal of Bioscience and Bioengineering}, pages={178--179}, author={Chami, Mohamed and Franke, Barbara and Mayans, Olga and Kreplak, Laurent} }
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