jYCaMP : an optimized calcium indicator for two-photon imaging at fiber laser wavelengths

Kurzanzeige
dc.contributor.authorMohr, Manuel Alexander
dc.contributor.authorBushey, Daniel
dc.contributor.authorAggarwal, Abhi
dc.contributor.authorMarvin, Jonathan S.
dc.contributor.authorKim, Jeong Jun
dc.contributor.authorMarquez, Emiliano Jimenez
dc.contributor.authorLiang, Yajie
dc.contributor.authorPatel, Ronak
dc.contributor.authorLee, Chi-Yu
dc.contributor.authorPodgorski, Kaspar
dc.date.accessioned2025-05-26T09:56:11Z
dc.date.available2025-05-26T09:56:11Z
dc.date.issued2020-07
dc.description.abstractFemtosecond lasers at fixed wavelengths above 1,000 nm are powerful, stable and inexpensive, making them promising sources for two-photon microscopy. Biosensors optimized for these wavelengths are needed for both next-generation microscopes and affordable turn-key systems. Here we report jYCaMP1, a yellow variant of the calcium indicator jGCaMP7 that outperforms its parent in mice and flies at excitation wavelengths above 1,000 nm and enables improved two-color calcium imaging with red fluorescent protein-based indicators.
dc.description.versionpublisheddeu
dc.identifier.doi10.1038/s41592-020-0835-7
dc.identifier.urihttps://kops.uni-konstanz.de/handle/123456789/73439
dc.language.isoeng
dc.subjectFluorescence imaging
dc.subjectFluorescent proteins
dc.subject.ddc570
dc.titlejYCaMP : an optimized calcium indicator for two-photon imaging at fiber laser wavelengthseng
dc.typeJOURNAL_ARTICLE
dspace.entity.typePublication
kops.citation.bibtex
@article{Mohr2020-07jYCaM-73439,
  title={jYCaMP : an optimized calcium indicator for two-photon imaging at fiber laser wavelengths},
  year={2020},
  doi={10.1038/s41592-020-0835-7},
  number={7},
  volume={17},
  issn={1548-7091},
  journal={Nature Methods},
  pages={694--697},
  author={Mohr, Manuel Alexander and Bushey, Daniel and Aggarwal, Abhi and Marvin, Jonathan S. and Kim, Jeong Jun and Marquez, Emiliano Jimenez and Liang, Yajie and Patel, Ronak and Lee, Chi-Yu and Podgorski, Kaspar}
}
kops.citation.iso690MOHR, Manuel Alexander, Daniel BUSHEY, Abhi AGGARWAL, Jonathan S. MARVIN, Jeong Jun KIM, Emiliano Jimenez MARQUEZ, Yajie LIANG, Ronak PATEL, Chi-Yu LEE, Kaspar PODGORSKI, 2020. jYCaMP : an optimized calcium indicator for two-photon imaging at fiber laser wavelengths. In: Nature Methods. Springer. 2020, 17(7), S. 694-697. ISSN 1548-7091. eISSN 1548-7105. Verfügbar unter: doi: 10.1038/s41592-020-0835-7deu
kops.citation.iso690MOHR, Manuel Alexander, Daniel BUSHEY, Abhi AGGARWAL, Jonathan S. MARVIN, Jeong Jun KIM, Emiliano Jimenez MARQUEZ, Yajie LIANG, Ronak PATEL, Chi-Yu LEE, Kaspar PODGORSKI, 2020. jYCaMP : an optimized calcium indicator for two-photon imaging at fiber laser wavelengths. In: Nature Methods. Springer. 2020, 17(7), pp. 694-697. ISSN 1548-7091. eISSN 1548-7105. Available under: doi: 10.1038/s41592-020-0835-7eng
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